Genomic comparisons and phylogenetic analysis of mastitis‑related staphylococci with a focus on adhesion, bioflm, and related regulatory genes.
Mastitis is a common and costly disease on dairy farms, commonly caused by Staphylococcus spp. though the various species are associated with different clinical outcomes. In the current study, we performed genomic analyses to determine the prevalence of adhesion, biofilm, and related regulatory genes in 478 staphylococcal species isolated from clinical and subclinical mastitis cases deposited in public databases. The most prevalent adhesin genes (ebpS, atl, pls, sasH and sasF) were found in both clinical and subclinical isolates. However, the ebpS gene was absent in subclinical isolates of Staphylococcus arlettae, S. succinus, S. sciuri, S. equorun, S. galinarum, and S. saprophyticus. In contrast, the coa, eap, emp, efb, and vWbp genes were present more frequently in clinical (vs. subclincal) mastitis isolates and were highly correlated with the presence of the biofim operon (icaABCD) and its transcriptional regulator, icaR. Co-phylogenetic analyses suggested that many of these adhesins, biofilm, and associated regulatory genes could have been horizontally disseminated between clinical and subclinical isolates. Our results further suggest that several adhesins, biofilm, and related regulatory genes, which have been overlooked in previous studies, may be of use for virulence profiling of mastitis-related Staphylococcus strains or as potential targets for vaccine development.
Main Authors: | , , , , , , , |
---|---|
Other Authors: | |
Format: | Artigo de periódico biblioteca |
Language: | Ingles English |
Published: |
2021-12-07
|
Subjects: | Biofim operon, IcaABCD, IcaR, Regulatory genes, Mastitis, Staphylococcus, Staphylococcus arlettae, Staphylococcus succinus, Staphylococcus sciuri, Staphylococcus equorum, Staphylococcus gallinarum, Staphylococcus saprophyticus, Adhesins, Vaccine development, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1137199 https://doi.org/10.1038/s41598-021-96842-2 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-alice-doc-1137199 |
---|---|
record_format |
koha |
spelling |
dig-alice-doc-11371992021-12-07T12:01:12Z Genomic comparisons and phylogenetic analysis of mastitis‑related staphylococci with a focus on adhesion, bioflm, and related regulatory genes. PIZAURO, L. J. L. ALMEIDA, C. C. DE SILVA, S. R. MACINNES, J. I. KROPINSKI, A. M. ZAFALON, L. F. AVILA, F. A. DE VARANI, A. DE M. LUCAS JOSÉ LUDUVERIO PIZAURO, UNESP; CAMILA CHIODA DE ALMEIDA, UNESP; SAURA RODRIGUES SILVA, UNESP; JANET I. MACINNES, UNIVERSITY OF GUELPH; ANDREW M. KROPINSKI, UNIVERSITY OF GUELPH; LUIZ FRANCISCO ZAFALON, CPPSE; FERNANDO ANTÔNIO DE AVILA, UNESP; ALESSANDRO DE MELLO VARANI, UNESP. Biofim operon IcaABCD IcaR Regulatory genes Mastitis Staphylococcus Staphylococcus arlettae Staphylococcus succinus Staphylococcus sciuri Staphylococcus equorum Staphylococcus gallinarum Staphylococcus saprophyticus Adhesins Vaccine development Mastitis is a common and costly disease on dairy farms, commonly caused by Staphylococcus spp. though the various species are associated with different clinical outcomes. In the current study, we performed genomic analyses to determine the prevalence of adhesion, biofilm, and related regulatory genes in 478 staphylococcal species isolated from clinical and subclinical mastitis cases deposited in public databases. The most prevalent adhesin genes (ebpS, atl, pls, sasH and sasF) were found in both clinical and subclinical isolates. However, the ebpS gene was absent in subclinical isolates of Staphylococcus arlettae, S. succinus, S. sciuri, S. equorun, S. galinarum, and S. saprophyticus. In contrast, the coa, eap, emp, efb, and vWbp genes were present more frequently in clinical (vs. subclincal) mastitis isolates and were highly correlated with the presence of the biofim operon (icaABCD) and its transcriptional regulator, icaR. Co-phylogenetic analyses suggested that many of these adhesins, biofilm, and associated regulatory genes could have been horizontally disseminated between clinical and subclinical isolates. Our results further suggest that several adhesins, biofilm, and related regulatory genes, which have been overlooked in previous studies, may be of use for virulence profiling of mastitis-related Staphylococcus strains or as potential targets for vaccine development. 2021-12-07T12:01:03Z 2021-12-07T12:01:03Z 2021-12-07 2021 Artigo de periódico Scientific Reports, v.11, 2021, 17392. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1137199 https://doi.org/10.1038/s41598-021-96842-2 Ingles en openAccess 10 p. |
institution |
EMBRAPA |
collection |
DSpace |
country |
Brasil |
countrycode |
BR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-alice |
tag |
biblioteca |
region |
America del Sur |
libraryname |
Sistema de bibliotecas de EMBRAPA |
language |
Ingles English |
topic |
Biofim operon IcaABCD IcaR Regulatory genes Mastitis Staphylococcus Staphylococcus arlettae Staphylococcus succinus Staphylococcus sciuri Staphylococcus equorum Staphylococcus gallinarum Staphylococcus saprophyticus Adhesins Vaccine development Biofim operon IcaABCD IcaR Regulatory genes Mastitis Staphylococcus Staphylococcus arlettae Staphylococcus succinus Staphylococcus sciuri Staphylococcus equorum Staphylococcus gallinarum Staphylococcus saprophyticus Adhesins Vaccine development |
spellingShingle |
Biofim operon IcaABCD IcaR Regulatory genes Mastitis Staphylococcus Staphylococcus arlettae Staphylococcus succinus Staphylococcus sciuri Staphylococcus equorum Staphylococcus gallinarum Staphylococcus saprophyticus Adhesins Vaccine development Biofim operon IcaABCD IcaR Regulatory genes Mastitis Staphylococcus Staphylococcus arlettae Staphylococcus succinus Staphylococcus sciuri Staphylococcus equorum Staphylococcus gallinarum Staphylococcus saprophyticus Adhesins Vaccine development PIZAURO, L. J. L. ALMEIDA, C. C. DE SILVA, S. R. MACINNES, J. I. KROPINSKI, A. M. ZAFALON, L. F. AVILA, F. A. DE VARANI, A. DE M. Genomic comparisons and phylogenetic analysis of mastitis‑related staphylococci with a focus on adhesion, bioflm, and related regulatory genes. |
description |
Mastitis is a common and costly disease on dairy farms, commonly caused by Staphylococcus spp. though the various species are associated with different clinical outcomes. In the current study, we performed genomic analyses to determine the prevalence of adhesion, biofilm, and related regulatory genes in 478 staphylococcal species isolated from clinical and subclinical mastitis cases deposited in public databases. The most prevalent adhesin genes (ebpS, atl, pls, sasH and sasF) were found in both clinical and subclinical isolates. However, the ebpS gene was absent in subclinical isolates of Staphylococcus arlettae, S. succinus, S. sciuri, S. equorun, S. galinarum, and S. saprophyticus. In contrast, the coa, eap, emp, efb, and vWbp genes were present more frequently in clinical (vs. subclincal) mastitis isolates and were highly correlated with the presence of the biofim operon (icaABCD) and its transcriptional regulator, icaR. Co-phylogenetic analyses suggested that many of these adhesins, biofilm, and associated regulatory genes could have been horizontally disseminated between clinical and subclinical isolates. Our results further suggest that several adhesins, biofilm, and related regulatory genes, which have been overlooked in previous studies, may be of use for virulence profiling of mastitis-related Staphylococcus strains or as potential targets for vaccine development. |
author2 |
LUCAS JOSÉ LUDUVERIO PIZAURO, UNESP; CAMILA CHIODA DE ALMEIDA, UNESP; SAURA RODRIGUES SILVA, UNESP; JANET I. MACINNES, UNIVERSITY OF GUELPH; ANDREW M. KROPINSKI, UNIVERSITY OF GUELPH; LUIZ FRANCISCO ZAFALON, CPPSE; FERNANDO ANTÔNIO DE AVILA, UNESP; ALESSANDRO DE MELLO VARANI, UNESP. |
author_facet |
LUCAS JOSÉ LUDUVERIO PIZAURO, UNESP; CAMILA CHIODA DE ALMEIDA, UNESP; SAURA RODRIGUES SILVA, UNESP; JANET I. MACINNES, UNIVERSITY OF GUELPH; ANDREW M. KROPINSKI, UNIVERSITY OF GUELPH; LUIZ FRANCISCO ZAFALON, CPPSE; FERNANDO ANTÔNIO DE AVILA, UNESP; ALESSANDRO DE MELLO VARANI, UNESP. PIZAURO, L. J. L. ALMEIDA, C. C. DE SILVA, S. R. MACINNES, J. I. KROPINSKI, A. M. ZAFALON, L. F. AVILA, F. A. DE VARANI, A. DE M. |
format |
Artigo de periódico |
topic_facet |
Biofim operon IcaABCD IcaR Regulatory genes Mastitis Staphylococcus Staphylococcus arlettae Staphylococcus succinus Staphylococcus sciuri Staphylococcus equorum Staphylococcus gallinarum Staphylococcus saprophyticus Adhesins Vaccine development |
author |
PIZAURO, L. J. L. ALMEIDA, C. C. DE SILVA, S. R. MACINNES, J. I. KROPINSKI, A. M. ZAFALON, L. F. AVILA, F. A. DE VARANI, A. DE M. |
author_sort |
PIZAURO, L. J. L. |
title |
Genomic comparisons and phylogenetic analysis of mastitis‑related staphylococci with a focus on adhesion, bioflm, and related regulatory genes. |
title_short |
Genomic comparisons and phylogenetic analysis of mastitis‑related staphylococci with a focus on adhesion, bioflm, and related regulatory genes. |
title_full |
Genomic comparisons and phylogenetic analysis of mastitis‑related staphylococci with a focus on adhesion, bioflm, and related regulatory genes. |
title_fullStr |
Genomic comparisons and phylogenetic analysis of mastitis‑related staphylococci with a focus on adhesion, bioflm, and related regulatory genes. |
title_full_unstemmed |
Genomic comparisons and phylogenetic analysis of mastitis‑related staphylococci with a focus on adhesion, bioflm, and related regulatory genes. |
title_sort |
genomic comparisons and phylogenetic analysis of mastitis‑related staphylococci with a focus on adhesion, bioflm, and related regulatory genes. |
publishDate |
2021-12-07 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1137199 https://doi.org/10.1038/s41598-021-96842-2 |
work_keys_str_mv |
AT pizauroljl genomiccomparisonsandphylogeneticanalysisofmastitis8209relatedstaphylococciwithafocusonadhesionbioflmandrelatedregulatorygenes AT almeidaccde genomiccomparisonsandphylogeneticanalysisofmastitis8209relatedstaphylococciwithafocusonadhesionbioflmandrelatedregulatorygenes AT silvasr genomiccomparisonsandphylogeneticanalysisofmastitis8209relatedstaphylococciwithafocusonadhesionbioflmandrelatedregulatorygenes AT macinnesji genomiccomparisonsandphylogeneticanalysisofmastitis8209relatedstaphylococciwithafocusonadhesionbioflmandrelatedregulatorygenes AT kropinskiam genomiccomparisonsandphylogeneticanalysisofmastitis8209relatedstaphylococciwithafocusonadhesionbioflmandrelatedregulatorygenes AT zafalonlf genomiccomparisonsandphylogeneticanalysisofmastitis8209relatedstaphylococciwithafocusonadhesionbioflmandrelatedregulatorygenes AT avilafade genomiccomparisonsandphylogeneticanalysisofmastitis8209relatedstaphylococciwithafocusonadhesionbioflmandrelatedregulatorygenes AT varaniadem genomiccomparisonsandphylogeneticanalysisofmastitis8209relatedstaphylococciwithafocusonadhesionbioflmandrelatedregulatorygenes |
_version_ |
1756027861850914816 |