Salvinia auriculata: chemical profle and biological activity against Staphylococcus aureus isolated from bovine mastitis.

The aquatic plant Salvinia auriculata has been shown to possess promising properties for the treatment of Staphylococcus aureus bovine mastitis. The disease afects cattle health and compromises dairy cattle productivity, resulting in reduced milk production and higher mortality rates. The aim of this study was to evaluate the antimicrobial activity, antibioflm activity, and toxicity of S. auriculata root extracts using bovine mammary epithelial cells (MAC-T); determine the chemical composition of the most active extract; and develop an S. auriculata antiseptic solution for pre- and post-milking teat disinfection. Plants were collected during the four seasons of the year. The most active hexane extract was subjected to bioguided fractionation, which resulted in the isolation of six known compounds, stigmast-22-ene-3,6-dione, stigmasterol, friedelinol, ?-sitosterol, octadecyl alcohol, and octadecanoic acid. The antimicrobial and antibioflm activities of the most active extract and isolated compounds were determined against nine S. aureus strains isolated from cows with mastitis. The efcacy of the S. auriculata teat dip formulation was tested using an excised teat model (ex vivo), and promising results were obtained. The S. auriculata extract formulation proved to be as efective as commercial antimicrobials in reducing log counts in excised teats.

Bibliographic Details

Main Authors:	PURGATO, G. A., LIMA, S., BAETA, J. V. P. B., PIZZIOLO, V. R., SOUZA, G. N. de, DIAZ-MUÑOZ, G., DIAZ, M. A. N.
Other Authors:	GISLAINE APARECIDA PURGATO, Universidade Federal de Viçosa; SÂMIA LIMA, Universidade Federal de Viçosa; JEFERSON VICTOR PAULA BARROS BAETA, Fundação Ezequiel Dias; VIRGÍNIA RAMOS PIZZIOLO, Universidade Federal de Viçosa; GUILHERME NUNES DE SOUZA, CNPGL; GASPAR DIAZ-MUÑOZ, Universidade Federal de Minas Gerais; MARISA ALVES NOGUEIRA DIAZ, Universidade Federal de Viçosa.
Format:	Artigo de periódico biblioteca
Language:	Ingles English
Published:	2021-09-24
Subjects:	Atividade antimicrobiana, Formulação anti-séptica, Fitosterol, Atividade biológica, Perfil químico, Mastite, Bovino, Bactéria,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1134757 https://doi.org/10.1007/s42770-021-00595-z
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