Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss.
Valuable female cattle are continuously subject to follicular puncture (ovum pick-up - OPU). This technique is commonly used for in-vitro embryo production, but may result in ovarian lesion. Mesenchymal stem cells (MSC) ameliorate the function of injured tissues, but their use to treat ovarian lesions in cattle has not been established. We investigated whether a local injection of MSC would reduce the negative efects of repeated OPU under acute and chronic scenarios in bovines. First, we performed four OPU sessions and injected 2.5×106 MSCs immediately after the 4th OPU procedure (n=5). The treated organs (right ovary) were compared to their saline-treated counterparts (left), and presented superior production of oocytes and embryos in the three following OPU sessions (P<0.05). Then, cows with progressive fertility loss went through three OPU sessions. Animals received MSC, saline, or MSC+FSH in both ovaries after the frst OPU. In the two following OPU sessions, the MSC and MSC +FSH - treated groups failed to present any signifcant alteration in the number of oocytes and embryos compared to saline-treated animals. Thus, MSC have benefcial efects on the fertility of OPUlesioned cows, but not in cows with cystic ovarian disease and chronic ovarian lesions.
Main Authors: | , , , , , , , , , , |
---|---|
Other Authors: | |
Format: | Artigo de periódico biblioteca |
Language: | Ingles English |
Published: |
2021-08-09
|
Subjects: | Ovum pick-up, OPU, Mesenchymal stem cells, MSC, Fertility, Fertilização in-vitro, Bovino, Fertilidade Animal, Biotecnologia, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133327 https://doi.org/10.1038/s41598-020-64810-x |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-alice-doc-1133327 |
---|---|
record_format |
koha |
spelling |
dig-alice-doc-11333272021-08-10T13:00:35Z Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss. MALARD, P. F. PEIXER, M. A. S. GRAZIA, J. G. BRUNEL, H. dos S. S. FERES, L. F. VILLARROEL, C. L. SIQUEIRA, L. G. B. DODE, M. A. N. POGUE, R. VIANA, J. H. M. CARVALHO, J. L. PATRICIA F. MALARD, UCB; MAURICIO A. S. PEIXER, UCB; JOAO G. GRAZIA, CENATTE EMBRIÕES; HILANA DOS SANTOS SENA BRUNEL, BIO BIOTECNOLOGIA DA REPRODUÇÃO ANIMAL; LUIZ F. FERES, UNIFAL; CARLA L. VILLARROEL, BIO BIOTECNOLOGIA DA REPRODUÇÃO ANIMAL; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; MARGOT ALVES NUNES DODE, Cenargen; ROBERT POGUE, UCB; JOAO HENRIQUE MOREIRA VIANA, Cenargen; JULIANA L. CARVALHO, UCB. Ovum pick-up OPU Mesenchymal stem cells MSC Fertility Fertilização in-vitro Bovino Fertilidade Animal Biotecnologia Valuable female cattle are continuously subject to follicular puncture (ovum pick-up - OPU). This technique is commonly used for in-vitro embryo production, but may result in ovarian lesion. Mesenchymal stem cells (MSC) ameliorate the function of injured tissues, but their use to treat ovarian lesions in cattle has not been established. We investigated whether a local injection of MSC would reduce the negative efects of repeated OPU under acute and chronic scenarios in bovines. First, we performed four OPU sessions and injected 2.5×106 MSCs immediately after the 4th OPU procedure (n=5). The treated organs (right ovary) were compared to their saline-treated counterparts (left), and presented superior production of oocytes and embryos in the three following OPU sessions (P<0.05). Then, cows with progressive fertility loss went through three OPU sessions. Animals received MSC, saline, or MSC+FSH in both ovaries after the frst OPU. In the two following OPU sessions, the MSC and MSC +FSH - treated groups failed to present any signifcant alteration in the number of oocytes and embryos compared to saline-treated animals. Thus, MSC have benefcial efects on the fertility of OPUlesioned cows, but not in cows with cystic ovarian disease and chronic ovarian lesions. 2021-08-10T13:00:24Z 2021-08-10T13:00:24Z 2021-08-09 2020 Artigo de periódico Scientific Reports, v. 10, article 8018, 2020. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133327 https://doi.org/10.1038/s41598-020-64810-x Ingles en openAccess |
institution |
EMBRAPA |
collection |
DSpace |
country |
Brasil |
countrycode |
BR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-alice |
tag |
biblioteca |
region |
America del Sur |
libraryname |
Sistema de bibliotecas de EMBRAPA |
language |
Ingles English |
topic |
Ovum pick-up OPU Mesenchymal stem cells MSC Fertility Fertilização in-vitro Bovino Fertilidade Animal Biotecnologia Ovum pick-up OPU Mesenchymal stem cells MSC Fertility Fertilização in-vitro Bovino Fertilidade Animal Biotecnologia |
spellingShingle |
Ovum pick-up OPU Mesenchymal stem cells MSC Fertility Fertilização in-vitro Bovino Fertilidade Animal Biotecnologia Ovum pick-up OPU Mesenchymal stem cells MSC Fertility Fertilização in-vitro Bovino Fertilidade Animal Biotecnologia MALARD, P. F. PEIXER, M. A. S. GRAZIA, J. G. BRUNEL, H. dos S. S. FERES, L. F. VILLARROEL, C. L. SIQUEIRA, L. G. B. DODE, M. A. N. POGUE, R. VIANA, J. H. M. CARVALHO, J. L. Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss. |
description |
Valuable female cattle are continuously subject to follicular puncture (ovum pick-up - OPU). This technique is commonly used for in-vitro embryo production, but may result in ovarian lesion. Mesenchymal stem cells (MSC) ameliorate the function of injured tissues, but their use to treat ovarian lesions in cattle has not been established. We investigated whether a local injection of MSC would reduce the negative efects of repeated OPU under acute and chronic scenarios in bovines. First, we performed four OPU sessions and injected 2.5×106 MSCs immediately after the 4th OPU procedure (n=5). The treated organs (right ovary) were compared to their saline-treated counterparts (left), and presented superior production of oocytes and embryos in the three following OPU sessions (P<0.05). Then, cows with progressive fertility loss went through three OPU sessions. Animals received MSC, saline, or MSC+FSH in both ovaries after the frst OPU. In the two following OPU sessions, the MSC and MSC +FSH - treated groups failed to present any signifcant alteration in the number of oocytes and embryos compared to saline-treated animals. Thus, MSC have benefcial efects on the fertility of OPUlesioned cows, but not in cows with cystic ovarian disease and chronic ovarian lesions. |
author2 |
PATRICIA F. MALARD, UCB; MAURICIO A. S. PEIXER, UCB; JOAO G. GRAZIA, CENATTE EMBRIÕES; HILANA DOS SANTOS SENA BRUNEL, BIO BIOTECNOLOGIA DA REPRODUÇÃO ANIMAL; LUIZ F. FERES, UNIFAL; CARLA L. VILLARROEL, BIO BIOTECNOLOGIA DA REPRODUÇÃO ANIMAL; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; MARGOT ALVES NUNES DODE, Cenargen; ROBERT POGUE, UCB; JOAO HENRIQUE MOREIRA VIANA, Cenargen; JULIANA L. CARVALHO, UCB. |
author_facet |
PATRICIA F. MALARD, UCB; MAURICIO A. S. PEIXER, UCB; JOAO G. GRAZIA, CENATTE EMBRIÕES; HILANA DOS SANTOS SENA BRUNEL, BIO BIOTECNOLOGIA DA REPRODUÇÃO ANIMAL; LUIZ F. FERES, UNIFAL; CARLA L. VILLARROEL, BIO BIOTECNOLOGIA DA REPRODUÇÃO ANIMAL; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; MARGOT ALVES NUNES DODE, Cenargen; ROBERT POGUE, UCB; JOAO HENRIQUE MOREIRA VIANA, Cenargen; JULIANA L. CARVALHO, UCB. MALARD, P. F. PEIXER, M. A. S. GRAZIA, J. G. BRUNEL, H. dos S. S. FERES, L. F. VILLARROEL, C. L. SIQUEIRA, L. G. B. DODE, M. A. N. POGUE, R. VIANA, J. H. M. CARVALHO, J. L. |
format |
Artigo de periódico |
topic_facet |
Ovum pick-up OPU Mesenchymal stem cells MSC Fertility Fertilização in-vitro Bovino Fertilidade Animal Biotecnologia |
author |
MALARD, P. F. PEIXER, M. A. S. GRAZIA, J. G. BRUNEL, H. dos S. S. FERES, L. F. VILLARROEL, C. L. SIQUEIRA, L. G. B. DODE, M. A. N. POGUE, R. VIANA, J. H. M. CARVALHO, J. L. |
author_sort |
MALARD, P. F. |
title |
Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss. |
title_short |
Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss. |
title_full |
Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss. |
title_fullStr |
Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss. |
title_full_unstemmed |
Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss. |
title_sort |
intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss. |
publishDate |
2021-08-09 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133327 https://doi.org/10.1038/s41598-020-64810-x |
work_keys_str_mv |
AT malardpf intraovarianinjectionofmesenchymalstemcellsimprovesoocyteyieldandinvitroembryoproductioninabovinemodeloffertilityloss AT peixermas intraovarianinjectionofmesenchymalstemcellsimprovesoocyteyieldandinvitroembryoproductioninabovinemodeloffertilityloss AT graziajg intraovarianinjectionofmesenchymalstemcellsimprovesoocyteyieldandinvitroembryoproductioninabovinemodeloffertilityloss AT brunelhdosss intraovarianinjectionofmesenchymalstemcellsimprovesoocyteyieldandinvitroembryoproductioninabovinemodeloffertilityloss AT fereslf intraovarianinjectionofmesenchymalstemcellsimprovesoocyteyieldandinvitroembryoproductioninabovinemodeloffertilityloss AT villarroelcl intraovarianinjectionofmesenchymalstemcellsimprovesoocyteyieldandinvitroembryoproductioninabovinemodeloffertilityloss AT siqueiralgb intraovarianinjectionofmesenchymalstemcellsimprovesoocyteyieldandinvitroembryoproductioninabovinemodeloffertilityloss AT dodeman intraovarianinjectionofmesenchymalstemcellsimprovesoocyteyieldandinvitroembryoproductioninabovinemodeloffertilityloss AT poguer intraovarianinjectionofmesenchymalstemcellsimprovesoocyteyieldandinvitroembryoproductioninabovinemodeloffertilityloss AT vianajhm intraovarianinjectionofmesenchymalstemcellsimprovesoocyteyieldandinvitroembryoproductioninabovinemodeloffertilityloss AT carvalhojl intraovarianinjectionofmesenchymalstemcellsimprovesoocyteyieldandinvitroembryoproductioninabovinemodeloffertilityloss |
_version_ |
1756027639438508032 |