The resistance of the cowpea cv. BRS Xiquexique to infestation by cowpea weevil is related to the presence of toxic chitin-binding proteins.

The cowpea weevil (Callosobruchus maculatus) is the main pest that attacks cowpea (Vigna unguiculata) seedsb during storage, causing nutritional and economic losses in the cowpea crop. Thus, studies aiming to identify resistant cowpea cultivars have been developed. Chitin-binding proteins (CBP), such vicilins and chitinases, have been detected in seeds and related with the toxicity to insects. In this work, we investigated the presence of chitin-binding proteins in the partially resistant cowpea cv. BRS Xiquexique and evaluated their toxicity towards cowpea weevil. The CBP fraction was isolated by chitin affinity chromatography. CBP fraction showed, through 15% SDS PAGE, protein bands with varying molecular masses, mainly below 55 kDa.

Bibliographic Details

Main Authors:	FERREIRA, S. R., ROCHA, M. de M., SILVA, K. J. D. e, FERREIRA, A. T. S., PERALES, J., FERNANDES, K. V. S., OLIVEIRA, A. E. A.
Other Authors:	SARAH RODRIGUES FERREIRA, Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF, Campos dos Goytacazes, RJ, Brazil; MAURISRAEL DE MOURA ROCHA, CPAMN; KAESEL JACKSON DAMASCENO E SILVA, CPAMN; ANDRE T. S. FERREIRA, FIOCRUZ, Rio de Janeiro, RJ, Brazil; JONAS PERALES, FIOCRUZ, Rio de Janeiro, RJ, Brazil; KÁTIA V. S. FERNANDES, Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF, Campos dos Goytacazes, RJ, Brazil; ANTONIA E. A. OLIVEIRA, Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF, Campos dos Goytacazes, RJ, Brazil.
Format:	Artigo de periódico biblioteca
Language:	Ingles English
Published:	2021-05-18
Subjects:	Cultivares resistentes, Proteínas de defesa, Vicilins, Vigna Unguiculata, Callosobruchus Maculatus, Chitinase,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1131857 https://doi.org/10.1016/j.pestbp.2021.104782
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