Anthelmintic effect of Pterogyne nitens (Fabaceae) on eggs and larvae of Haemonchus contortus: analyses of structure-activity relationships based on phenolic compounds.
Due to high prevalence and large pathogenicity, Haemonchus contortus is the main gastrointestinal nematode in tropical and subtropical regions. This species is responsible for severe economic losses to sheep and goat breeders in Brazil. The control of this parasite is currently compromised, mainly, due to anthelmintic resistance. In the search for natural anthelmintic alternatives, Pterogyne nitens, a native Brazilian tree with potential ethnopharmacological activity, has been identified. The aim of this study was to evaluate the anthelmintic activity of ethanolic extracts and phenolic compounds from P. nitens, as well as two commercial flavonoids (chrysin and morin), to derive the chemical structure and anthelmintic activity. The ovicidal and larvicidal activity of ethanolic extracts from leaves (EEL) and fruits (EEFR), as well as natural compounds from P. nitens on H. contortus were evaluated through egg hatch assay (EHA) and larval development assay (LDA). The results showed that all extracts, especially the phenolic compounds were active in the EHA and LDA. The egg hatch inhibitory effects of EEL (EC50 = 316 μg/mL) were more potent than EEFR (EC50 = 512 μg/mL). However, larval development inhibitory effects of EEL (EC50 = 47 μg/mL) and EEFR (EC50 = 35 μg/mL) were similar. Among the compounds, the flavones (sorbifolin, pedalitin, and chrysin) did not have inhibitory effects on egg hatching but presented some activity against larval development of H. contortus. In contrast, the flavonols (quercetin, rutin, and morin) showed high activity in the EHA but were inactive in the LDA. The addition of at hydroxyl group and rutinose group to the flavonoid structure increased the ovicidal and larvicidal activity, respectively. The phenolic acids showed potent anthelmintic activity: caffeic acid, ferulic acid, and gallic acid had the highest anthelmintic effects, presenting EC50 values of 1.48, 0.56, and 4.93 μg/mL in the EHA; and 31, 22, and 33 μg/mL in the LDA, respectively. These results suggest that P. nitens might be a source of effective alternative compounds to control H. contortus.
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2021-03-08
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Subjects: | In vitro, Egg hatch assay (EHA), Larval development assay (LDA), Ethanolic extracts, Anthelmintics, Phenolic compounds, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1130547 |
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dig-alice-doc-11305472021-03-09T19:01:19Z Anthelmintic effect of Pterogyne nitens (Fabaceae) on eggs and larvae of Haemonchus contortus: analyses of structure-activity relationships based on phenolic compounds. LIMA, C. S. PEREIRA, M. H. ALEMÁN GAINZA, Y. HOSTE, H. REGASINI, L. O. CHAGAS, A. C. de S. CAROLINE SPRENGEL LIMA, UNESP; MATHEUS HENRIQUE PEREIRA, UNESP; YOUSMEL ALEMÁN GAINZA, CNPq; HERVÉ HOSTE, INRA; LUÍS OCTAVIO REGASINI, UNESP; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. In vitro Egg hatch assay (EHA) Larval development assay (LDA) Ethanolic extracts Anthelmintics Phenolic compounds Due to high prevalence and large pathogenicity, Haemonchus contortus is the main gastrointestinal nematode in tropical and subtropical regions. This species is responsible for severe economic losses to sheep and goat breeders in Brazil. The control of this parasite is currently compromised, mainly, due to anthelmintic resistance. In the search for natural anthelmintic alternatives, Pterogyne nitens, a native Brazilian tree with potential ethnopharmacological activity, has been identified. The aim of this study was to evaluate the anthelmintic activity of ethanolic extracts and phenolic compounds from P. nitens, as well as two commercial flavonoids (chrysin and morin), to derive the chemical structure and anthelmintic activity. The ovicidal and larvicidal activity of ethanolic extracts from leaves (EEL) and fruits (EEFR), as well as natural compounds from P. nitens on H. contortus were evaluated through egg hatch assay (EHA) and larval development assay (LDA). The results showed that all extracts, especially the phenolic compounds were active in the EHA and LDA. The egg hatch inhibitory effects of EEL (EC50 = 316 μg/mL) were more potent than EEFR (EC50 = 512 μg/mL). However, larval development inhibitory effects of EEL (EC50 = 47 μg/mL) and EEFR (EC50 = 35 μg/mL) were similar. Among the compounds, the flavones (sorbifolin, pedalitin, and chrysin) did not have inhibitory effects on egg hatching but presented some activity against larval development of H. contortus. In contrast, the flavonols (quercetin, rutin, and morin) showed high activity in the EHA but were inactive in the LDA. The addition of at hydroxyl group and rutinose group to the flavonoid structure increased the ovicidal and larvicidal activity, respectively. The phenolic acids showed potent anthelmintic activity: caffeic acid, ferulic acid, and gallic acid had the highest anthelmintic effects, presenting EC50 values of 1.48, 0.56, and 4.93 μg/mL in the EHA; and 31, 22, and 33 μg/mL in the LDA, respectively. These results suggest that P. nitens might be a source of effective alternative compounds to control H. contortus. 2021-03-09T19:01:10Z 2021-03-09T19:01:10Z 2021-03-08 2021 Artigo de periódico Industrial Crops and Products, v.164, june 2021, 113348. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1130547 Ingles en openAccess 8 p. |
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In vitro Egg hatch assay (EHA) Larval development assay (LDA) Ethanolic extracts Anthelmintics Phenolic compounds In vitro Egg hatch assay (EHA) Larval development assay (LDA) Ethanolic extracts Anthelmintics Phenolic compounds |
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In vitro Egg hatch assay (EHA) Larval development assay (LDA) Ethanolic extracts Anthelmintics Phenolic compounds In vitro Egg hatch assay (EHA) Larval development assay (LDA) Ethanolic extracts Anthelmintics Phenolic compounds LIMA, C. S. PEREIRA, M. H. ALEMÁN GAINZA, Y. HOSTE, H. REGASINI, L. O. CHAGAS, A. C. de S. Anthelmintic effect of Pterogyne nitens (Fabaceae) on eggs and larvae of Haemonchus contortus: analyses of structure-activity relationships based on phenolic compounds. |
description |
Due to high prevalence and large pathogenicity, Haemonchus contortus is the main gastrointestinal nematode in tropical and subtropical regions. This species is responsible for severe economic losses to sheep and goat breeders in Brazil. The control of this parasite is currently compromised, mainly, due to anthelmintic resistance. In the search for natural anthelmintic alternatives, Pterogyne nitens, a native Brazilian tree with potential ethnopharmacological activity, has been identified. The aim of this study was to evaluate the anthelmintic activity of ethanolic extracts and phenolic compounds from P. nitens, as well as two commercial flavonoids (chrysin and morin), to derive the chemical structure and anthelmintic activity. The ovicidal and larvicidal activity of ethanolic extracts from leaves (EEL) and fruits (EEFR), as well as natural compounds from P. nitens on H. contortus were evaluated through egg hatch assay (EHA) and larval development assay (LDA). The results showed that all extracts, especially the phenolic compounds were active in the EHA and LDA. The egg hatch inhibitory effects of EEL (EC50 = 316 μg/mL) were more potent than EEFR (EC50 = 512 μg/mL). However, larval development inhibitory effects of EEL (EC50 = 47 μg/mL) and EEFR (EC50 = 35 μg/mL) were similar. Among the compounds, the flavones (sorbifolin, pedalitin, and chrysin) did not have inhibitory effects on egg hatching but presented some activity against larval development of H. contortus. In contrast, the flavonols (quercetin, rutin, and morin) showed high activity in the EHA but were inactive in the LDA. The addition of at hydroxyl group and rutinose group to the flavonoid structure increased the ovicidal and larvicidal activity, respectively. The phenolic acids showed potent anthelmintic activity: caffeic acid, ferulic acid, and gallic acid had the highest anthelmintic effects, presenting EC50 values of 1.48, 0.56, and 4.93 μg/mL in the EHA; and 31, 22, and 33 μg/mL in the LDA, respectively. These results suggest that P. nitens might be a source of effective alternative compounds to control H. contortus. |
author2 |
CAROLINE SPRENGEL LIMA, UNESP; MATHEUS HENRIQUE PEREIRA, UNESP; YOUSMEL ALEMÁN GAINZA, CNPq; HERVÉ HOSTE, INRA; LUÍS OCTAVIO REGASINI, UNESP; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
author_facet |
CAROLINE SPRENGEL LIMA, UNESP; MATHEUS HENRIQUE PEREIRA, UNESP; YOUSMEL ALEMÁN GAINZA, CNPq; HERVÉ HOSTE, INRA; LUÍS OCTAVIO REGASINI, UNESP; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. LIMA, C. S. PEREIRA, M. H. ALEMÁN GAINZA, Y. HOSTE, H. REGASINI, L. O. CHAGAS, A. C. de S. |
format |
Artigo de periódico |
topic_facet |
In vitro Egg hatch assay (EHA) Larval development assay (LDA) Ethanolic extracts Anthelmintics Phenolic compounds |
author |
LIMA, C. S. PEREIRA, M. H. ALEMÁN GAINZA, Y. HOSTE, H. REGASINI, L. O. CHAGAS, A. C. de S. |
author_sort |
LIMA, C. S. |
title |
Anthelmintic effect of Pterogyne nitens (Fabaceae) on eggs and larvae of Haemonchus contortus: analyses of structure-activity relationships based on phenolic compounds. |
title_short |
Anthelmintic effect of Pterogyne nitens (Fabaceae) on eggs and larvae of Haemonchus contortus: analyses of structure-activity relationships based on phenolic compounds. |
title_full |
Anthelmintic effect of Pterogyne nitens (Fabaceae) on eggs and larvae of Haemonchus contortus: analyses of structure-activity relationships based on phenolic compounds. |
title_fullStr |
Anthelmintic effect of Pterogyne nitens (Fabaceae) on eggs and larvae of Haemonchus contortus: analyses of structure-activity relationships based on phenolic compounds. |
title_full_unstemmed |
Anthelmintic effect of Pterogyne nitens (Fabaceae) on eggs and larvae of Haemonchus contortus: analyses of structure-activity relationships based on phenolic compounds. |
title_sort |
anthelmintic effect of pterogyne nitens (fabaceae) on eggs and larvae of haemonchus contortus: analyses of structure-activity relationships based on phenolic compounds. |
publishDate |
2021-03-08 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1130547 |
work_keys_str_mv |
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