Co-inoculation of two symbiotically efcient Bradyrhizobium strains improves cowpea development better than a single bacterium application.

The co-inoculation of Bradyrhizobium with other non-bradyrhizobial strains was already assessed on cowpea, but the co-inoculation of two Bradyrhizobium strains was not tested up to now. This study aimed to evaluate the cowpea growth, N accumulation, and Bradyrhizobium competitiveness of the elite strain B. pachyrhizi BR 3262 when co-inoculated with other efcient Bradyrhizobium from the Brazilian semiarid region. Three potted-plant experiments were carried out. In the frst assay, 35 efcient Bradyrhizobium isolates obtained from the semiarid region of Brazil were co-inoculated with the elite strains B. pachyrhizi BR 3262. The experiment was conducted in gnotobiotic conditions. The plant growth, nodulation, N nutritional variables, and nodular occupation were assessed. Under gnotobiotic and non-sterile soil conditions, ten selected bacteria plus the elite strain B. yuanmingense BR 3267 were used at the second and third experiments, respectively. The cowpea was inoculated with the 11 bacteria individually or co-inoculated with BR 3262. The plant growth and N nutritional variables were assessed. A double-layer medium spot method experiment was conducted to evaluate the interaction among the co-inoculated strains in standard and diluted YMA media. The co-inoculation treatments showed the best efciency when compared to the treatments inoculated solely with BR 3262. This strain occupied a low amount of cowpea nodules ranging from 5 to 67.5%. The treatments with lower BR 3262 nodule occupancy showed the best results for the shoot nitrogen accumulation. The culture experiment showed that four bacteria inhibited the growth of BR 3262. In contrast, seven strains from the soils of Brazilian semiarid region were benefted by the previous inoculation of this strain. In the second and third experiments, the results indicated that all 11 co-inoculated treatments were more efcient than the single inoculation, proofng the best performance of the dual inoculation of Bradyrhizobium on cowpea.

Bibliographic Details

Main Authors:	NASCIMENTO, T. R. de, SENA, P. T. S., OLIVEIRA, G. S., SILVA, T. R. da, DIAS, M. A. M., FREITAS, A. D. S. de, MARTINS, L. M. V., FERNANDES JUNIOR, P. I.
Other Authors:	Tailane Ribeiro do Nascimento; Pâmella Thalita Souza Sena; Gilmar Silva Oliveira; Thaise Rosa da Silva; Marcos André Moura Dias; Ana Dolores Santiago de Freitas; Lindete Míria Vieira Martins; PAULO IVAN FERNANDES JUNIOR, CPATSA.
Format:	Artigo de periódico biblioteca
Language:	Ingles English
Published:	2021-01-12
Subjects:	Fxação biológica de nitrogênio, Inoculação de Dual, Seleção de cepas, Feijão caupi, Feijão, Inoculante, Vigna Unguiculata, Bradyrhizobium, Cowpeas,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1129244 https://doi.org/10.1007/s13205-020-02534-5
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