Characterization and selection of ISSR molecular markers in species of Passiflora spp.
The objective to be achieved with the search for molecular markers of low cost and good reproducibility for the selection and genetic characterization of passion fruit was to select ISSR (Inter Simple Sequence Repeat) primers for different unimproved varieties and cultivars of Passiflora, in order to subsidize molecular genetic studies. To achieve this goal, 23 ISSR starters were tested for eight unimproved varieties and 12 cultivars of Passiflora spp. The amplifications were obtained by electrophoresis on 2% agarose gel, photocumented under transilluminator with ultraviolet light. The ISSR primers showed satisfactory results. Among the 23 primers, 16 obtained values above 60% of efficiency when classified as good (presence of two marks or more). As a consequence of the results presented, it can be attested that such primers are suitable for studies of polymorphism, structure and genetic diversity in species of the Passiflora genus, in addition to assisting conservation and breeding programs.
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Format: | Artigo de periódico biblioteca |
Language: | Portugues pt_BR |
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2020-12-28
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Subjects: | Marcador Molecular, Maracujá, Passifloraceae, Polimorfismo Genético, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1128773 |
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dig-alice-doc-11287732020-12-29T09:04:28Z Characterization and selection of ISSR molecular markers in species of Passiflora spp. DIAS, N. D. S. C. SOUZA, L. N. B. de SANTANA, V. de O. MEIRA, M. R. SANTOS, E. S. L. FALEIRO, F. G. SILVA, C. B. M. C. FABIO GELAPE FALEIRO, CPAC. Marcador Molecular Maracujá Passifloraceae Polimorfismo Genético The objective to be achieved with the search for molecular markers of low cost and good reproducibility for the selection and genetic characterization of passion fruit was to select ISSR (Inter Simple Sequence Repeat) primers for different unimproved varieties and cultivars of Passiflora, in order to subsidize molecular genetic studies. To achieve this goal, 23 ISSR starters were tested for eight unimproved varieties and 12 cultivars of Passiflora spp. The amplifications were obtained by electrophoresis on 2% agarose gel, photocumented under transilluminator with ultraviolet light. The ISSR primers showed satisfactory results. Among the 23 primers, 16 obtained values above 60% of efficiency when classified as good (presence of two marks or more). As a consequence of the results presented, it can be attested that such primers are suitable for studies of polymorphism, structure and genetic diversity in species of the Passiflora genus, in addition to assisting conservation and breeding programs. 2020-12-29T09:04:21Z 2020-12-29T09:04:21Z 2020-12-28 2020 Artigo de periódico Multi-Science Journal, v. 3, n. 3.2020. 2359-6902 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1128773 Portugues pt_BR openAccess |
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Marcador Molecular Maracujá Passifloraceae Polimorfismo Genético Marcador Molecular Maracujá Passifloraceae Polimorfismo Genético |
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Marcador Molecular Maracujá Passifloraceae Polimorfismo Genético Marcador Molecular Maracujá Passifloraceae Polimorfismo Genético DIAS, N. D. S. C. SOUZA, L. N. B. de SANTANA, V. de O. MEIRA, M. R. SANTOS, E. S. L. FALEIRO, F. G. SILVA, C. B. M. C. Characterization and selection of ISSR molecular markers in species of Passiflora spp. |
description |
The objective to be achieved with the search for molecular markers of low cost and good reproducibility for the selection and genetic characterization of passion fruit was to select ISSR (Inter Simple Sequence Repeat) primers for different unimproved varieties and cultivars of Passiflora, in order to subsidize molecular genetic studies. To achieve this goal, 23 ISSR starters were tested for eight unimproved varieties and 12 cultivars of Passiflora spp. The amplifications were obtained by electrophoresis on 2% agarose gel, photocumented under transilluminator with ultraviolet light. The ISSR primers showed satisfactory results. Among the 23 primers, 16 obtained values above 60% of efficiency when classified as good (presence of two marks or more). As a consequence of the results presented, it can be attested that such primers are suitable for studies of polymorphism, structure and genetic diversity in species of the Passiflora genus, in addition to assisting conservation and breeding programs. |
author2 |
FABIO GELAPE FALEIRO, CPAC. |
author_facet |
FABIO GELAPE FALEIRO, CPAC. DIAS, N. D. S. C. SOUZA, L. N. B. de SANTANA, V. de O. MEIRA, M. R. SANTOS, E. S. L. FALEIRO, F. G. SILVA, C. B. M. C. |
format |
Artigo de periódico |
topic_facet |
Marcador Molecular Maracujá Passifloraceae Polimorfismo Genético |
author |
DIAS, N. D. S. C. SOUZA, L. N. B. de SANTANA, V. de O. MEIRA, M. R. SANTOS, E. S. L. FALEIRO, F. G. SILVA, C. B. M. C. |
author_sort |
DIAS, N. D. S. C. |
title |
Characterization and selection of ISSR molecular markers in species of Passiflora spp. |
title_short |
Characterization and selection of ISSR molecular markers in species of Passiflora spp. |
title_full |
Characterization and selection of ISSR molecular markers in species of Passiflora spp. |
title_fullStr |
Characterization and selection of ISSR molecular markers in species of Passiflora spp. |
title_full_unstemmed |
Characterization and selection of ISSR molecular markers in species of Passiflora spp. |
title_sort |
characterization and selection of issr molecular markers in species of passiflora spp. |
publishDate |
2020-12-28 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1128773 |
work_keys_str_mv |
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1756027331652091904 |