Determination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in Serra Gaúcha, Brazil.
Among grapevine (Vitis spp.) pathogens, viruses stand out, considering that this fruit plant is broadly susceptible to them and that they cause several diseases, reducing quality and yield of grape production. However, detecting and identifying viral infections in grapevines can be challenging. The objectives of this study were to detect grapevine viral pathogens by high-throughput sequencing (HTS) and identify grapevine viruses associated with plant propagation material from different origins. A comprehensive picture of a vine exhibiting virus-related symptoms was generated. The detected virome included four different pathogens and their variants. In addition, the incidence of seven viral pathogens was determined in samples collected in three vineyards of the main grape-growing region of Brazil. It is suggested that the observed infections could be related to the technological level of the production system adopted by these grape growers. Keywords: RT-qPCR; HTS; NGS; Vitis; indexing; survey
Main Authors: | , , |
---|---|
Other Authors: | |
Format: | Artigo de periódico biblioteca |
Language: | Ingles English |
Published: |
2020-05-11
|
Subjects: | RT-qPCR, HTS, NGS, Indexing, Survey, Grapevine (Vitis spp), Vitis, Pathogens, Viruses, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1122194 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-alice-doc-1122194 |
---|---|
record_format |
koha |
spelling |
dig-alice-doc-11221942020-05-12T04:39:39Z Determination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in Serra Gaúcha, Brazil. FAJARDO, T. V. M. Bertocchi, A. A. NICKEL, O. THOR VINICIUS MARTINS FAJARDO, CNPUV; Airton Alexandre Bertocchi, Universidade Estadual do Rio Grande do Sul, Bento Gonçalves, Rio Grande do Sul, Brazil. airtonalebertocchi@gmail.com; OSMAR NICKEL, CNPUV. RT-qPCR HTS NGS Indexing Survey Grapevine (Vitis spp) Vitis Pathogens Viruses Among grapevine (Vitis spp.) pathogens, viruses stand out, considering that this fruit plant is broadly susceptible to them and that they cause several diseases, reducing quality and yield of grape production. However, detecting and identifying viral infections in grapevines can be challenging. The objectives of this study were to detect grapevine viral pathogens by high-throughput sequencing (HTS) and identify grapevine viruses associated with plant propagation material from different origins. A comprehensive picture of a vine exhibiting virus-related symptoms was generated. The detected virome included four different pathogens and their variants. In addition, the incidence of seven viral pathogens was determined in samples collected in three vineyards of the main grape-growing region of Brazil. It is suggested that the observed infections could be related to the technological level of the production system adopted by these grape growers. Keywords: RT-qPCR; HTS; NGS; Vitis; indexing; survey 2020-05-12T04:39:33Z 2020-05-12T04:39:33Z 2020-05-11 2020 Artigo de periódico Revista Ceres, Viçosa, v. 67, n. 2, p. 156-163, mar./apr. 2020. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1122194 10.1590/0034-737X202067020010 Ingles en openAccess |
institution |
EMBRAPA |
collection |
DSpace |
country |
Brasil |
countrycode |
BR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-alice |
tag |
biblioteca |
region |
America del Sur |
libraryname |
Sistema de bibliotecas de EMBRAPA |
language |
Ingles English |
topic |
RT-qPCR HTS NGS Indexing Survey Grapevine (Vitis spp) Vitis Pathogens Viruses RT-qPCR HTS NGS Indexing Survey Grapevine (Vitis spp) Vitis Pathogens Viruses |
spellingShingle |
RT-qPCR HTS NGS Indexing Survey Grapevine (Vitis spp) Vitis Pathogens Viruses RT-qPCR HTS NGS Indexing Survey Grapevine (Vitis spp) Vitis Pathogens Viruses FAJARDO, T. V. M. Bertocchi, A. A. NICKEL, O. Determination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in Serra Gaúcha, Brazil. |
description |
Among grapevine (Vitis spp.) pathogens, viruses stand out, considering that this fruit plant is broadly susceptible to them and that they cause several diseases, reducing quality and yield of grape production. However, detecting and identifying viral infections in grapevines can be challenging. The objectives of this study were to detect grapevine viral pathogens by high-throughput sequencing (HTS) and identify grapevine viruses associated with plant propagation material from different origins. A comprehensive picture of a vine exhibiting virus-related symptoms was generated. The detected virome included four different pathogens and their variants. In addition, the incidence of seven viral pathogens was determined in samples collected in three vineyards of the main grape-growing region of Brazil. It is suggested that the observed infections could be related to the technological level of the production system adopted by these grape growers. Keywords: RT-qPCR; HTS; NGS; Vitis; indexing; survey |
author2 |
THOR VINICIUS MARTINS FAJARDO, CNPUV; Airton Alexandre Bertocchi, Universidade Estadual do Rio Grande do Sul, Bento Gonçalves, Rio Grande do Sul, Brazil. airtonalebertocchi@gmail.com; OSMAR NICKEL, CNPUV. |
author_facet |
THOR VINICIUS MARTINS FAJARDO, CNPUV; Airton Alexandre Bertocchi, Universidade Estadual do Rio Grande do Sul, Bento Gonçalves, Rio Grande do Sul, Brazil. airtonalebertocchi@gmail.com; OSMAR NICKEL, CNPUV. FAJARDO, T. V. M. Bertocchi, A. A. NICKEL, O. |
format |
Artigo de periódico |
topic_facet |
RT-qPCR HTS NGS Indexing Survey Grapevine (Vitis spp) Vitis Pathogens Viruses |
author |
FAJARDO, T. V. M. Bertocchi, A. A. NICKEL, O. |
author_sort |
FAJARDO, T. V. M. |
title |
Determination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in Serra Gaúcha, Brazil. |
title_short |
Determination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in Serra Gaúcha, Brazil. |
title_full |
Determination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in Serra Gaúcha, Brazil. |
title_fullStr |
Determination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in Serra Gaúcha, Brazil. |
title_full_unstemmed |
Determination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in Serra Gaúcha, Brazil. |
title_sort |
determination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in serra gaúcha, brazil. |
publishDate |
2020-05-11 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1122194 |
work_keys_str_mv |
AT fajardotvm determinationofthegrapevineviromebyhighthroughputsequencingandgrapevinevirusesdetectioninserragauchabrazil AT bertocchiaa determinationofthegrapevineviromebyhighthroughputsequencingandgrapevinevirusesdetectioninserragauchabrazil AT nickelo determinationofthegrapevineviromebyhighthroughputsequencingandgrapevinevirusesdetectioninserragauchabrazil |
_version_ |
1756026961623252992 |