Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential.

Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential.

This study aimed to isolate and identify endophytic fungi from the forage grass P. maximum and evaluate their ability to inhibit the growth of plant pathogenic fungi. One sample from P. purpureum grass was also included. Surface disinfected stem fragments were used for endophytic fungal isolation. One hundred and twenty-six endophytic fungi were isolated, of which 118 were from P. maximum and eight from P. purpureum. Morphological characteristics and internal transcribed spacer (ITS) and 18S (NS) sequence comparisons identified most isolated endophytic fungi as belonging to the phylum Ascomycota, with Sarocladium being the dominant genus. The isolates were subjected to in vitro antagonism tests against pathogenic fungi, and 31 endophytic fungi inhibited the growth of Bipolaris maydis, Penicillium expansum, and Sclerotinia minor. The results expand our knowledge of the diversity of endophytes associated with tropical grasses and suggest that they may represent new sources of antifungal metabolites for biocontrol and biotechnological purposes.

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Bibliographic Details
Main Authors: MAIA, N. da C., SOUZA, P. N. da C., GODINHO, B. T. V., MOREIRA, S. I., ABREU, L. M. de, JANK, L., CARDOSO, P. G.
Other Authors: Natália da Costa Maia, Universidade Federal de Lavras - UFLA/Departamento de Biologia; Patrícia Nirlane da Costa Souza, Universidade Federal dos Vales do Jequitinhonha e Mucuri - UFVJM; Bárbara Temponi Vilarino Godinho, Universidade Federal de Lavras - UFLA/Departamento de Biologia; Silvino Intra Moreira, Universidade Federal de Lavras - UFLA/Departamento de Fitopatologia; Lucas Magalhães de Abreu, Universidade Federal de Viçosa - UFV/Departamento de Fitopatologia; LIANA JANK, CNPGC; Patrícia Gomes Cardoso, Universidade Federal de Lavras - UFLA/Departamento de Biologia.
Format: Artigo de periódico biblioteca
Language:English
eng
Published: 2019-07-04
Subjects:Antagonism, Molecular identification, RDNA, Grasses, Plant pathogens,
Online Access:http://www.alice.cnptia.embrapa.br/alice/handle/doc/1110371
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Summary:This study aimed to isolate and identify endophytic fungi from the forage grass P. maximum and evaluate their ability to inhibit the growth of plant pathogenic fungi. One sample from P. purpureum grass was also included. Surface disinfected stem fragments were used for endophytic fungal isolation. One hundred and twenty-six endophytic fungi were isolated, of which 118 were from P. maximum and eight from P. purpureum. Morphological characteristics and internal transcribed spacer (ITS) and 18S (NS) sequence comparisons identified most isolated endophytic fungi as belonging to the phylum Ascomycota, with Sarocladium being the dominant genus. The isolates were subjected to in vitro antagonism tests against pathogenic fungi, and 31 endophytic fungi inhibited the growth of Bipolaris maydis, Penicillium expansum, and Sclerotinia minor. The results expand our knowledge of the diversity of endophytes associated with tropical grasses and suggest that they may represent new sources of antifungal metabolites for biocontrol and biotechnological purposes.

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