Long-Term encapsulated nitrate supplementation modulates rumen microbial diversity and rumen fermentation to reduce methane emission in grazing steers.
This study investigated the long-term effects (13 months) of encapsulated nitrate supplementation (ENS) on enteric methane emissions, rumen fermentation parameters, ruminal bacteria, and diversity of archaea in grazing beef cattle. We used a total of thirty-two Nellore steers (initial BW of 197 15.3 kg), 12 of which were fitted with rumen cannulas. For 13 months, the animals were maintained in 12 paddocks and fed a concentrate of ground corn, soybean meals, mineral supplements, and urea (URS) or encapsulated nitrate (EN) containing 70 g of EN/100 kg of BW (corresponding to 47 g NO3/100 kg BW). Encapsulated nitrate supplementation resulted in similar forage, supplement and total DMI values as URS (P > 0.05), but ENS tended to increase (C48 g/d; P = 0.055) average daily weight gain. Daily reductions in methane emissions (- 9.54 g or 18.5%) were observed with ENS when expressed as g of CH4/kg of forage dry matter intake (fDMI) (P = 0.037). Lower concentrations of NH3-N and a higher ruminal pH were observed in ENS groups 6 h after supplementation (P < 0.05). Total VFA rumen concentration 6 h (P = 0.009) and 12 h after supplementation with EN resulted in lower acetate concentrations in the rumen (P = 0.041). Steers supplemented with EN had a greater ruminal abundance of Bacteroides, Barnesiella, Lactobacillus, Selenomonas, Veillonella, Succinimonas, Succinivibrio, and Duganella sp. (P < 0.05), but a lower abundance of Methanobrevibacter sp. (P = 0.007). Strong negative correlations were found between daily methane emissions and Proteobacteria, Erysipelotrichaceae, Prevotellaceae, and Roseburia, Kandleria, Selenomonas, Veillonella, and Succinivibrio sp. (P < 0.05) in the rumen of ENS steers. Encapsulated nitrate is a feed additive that persistently affects enteric methane emission in grazing steers, thereby decreasing Methanobrevibacter abundance in the rumen. In addition, ENS can promote fumarate-reducer and lactateproducer bacteria, thereby reducing acetate production during rumen fermentation.
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Format: | Artigo de periódico biblioteca |
Language: | English eng |
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2019-04-03
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Subjects: | Archaea diversity, Enteric methane emission, Gado de Corte, Nitrato, Beef cattle, Rumen bacteria, Fatty acids, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1107883 |
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dig-alice-doc-11078832019-04-04T00:36:35Z Long-Term encapsulated nitrate supplementation modulates rumen microbial diversity and rumen fermentation to reduce methane emission in grazing steers. GRANJA-SALCEDO, Y. T. FERNANDES, R. M. ARAUJO, R. C. de KISHI, L. T. BERCHIELLI, T. T. RESENDE, F. D. de BERNDT, A. SIQUEIRA, G. R. Yury Tatiana Granja-Salcedo, UNESP; Rodolfo Maciel Fernandes, UNESP/Agência Paulista de Tecnologia dos Agronegócios; Rafael Canonenco de Araujo, GRASP; Luciano Takeshi Kishi, UNESP; Telma Teresinha Berchielli, UNESP; Flávio Dutra de Resende, UNESP; ALEXANDRE BERNDT, CPPSE; Gustavo Rezende Siqueira, UNESP/Agência Paulista de Tecnologia dos Agronegócios. Archaea diversity Enteric methane emission Gado de Corte Nitrato Beef cattle Rumen bacteria Fatty acids This study investigated the long-term effects (13 months) of encapsulated nitrate supplementation (ENS) on enteric methane emissions, rumen fermentation parameters, ruminal bacteria, and diversity of archaea in grazing beef cattle. We used a total of thirty-two Nellore steers (initial BW of 197 15.3 kg), 12 of which were fitted with rumen cannulas. For 13 months, the animals were maintained in 12 paddocks and fed a concentrate of ground corn, soybean meals, mineral supplements, and urea (URS) or encapsulated nitrate (EN) containing 70 g of EN/100 kg of BW (corresponding to 47 g NO3/100 kg BW). Encapsulated nitrate supplementation resulted in similar forage, supplement and total DMI values as URS (P > 0.05), but ENS tended to increase (C48 g/d; P = 0.055) average daily weight gain. Daily reductions in methane emissions (- 9.54 g or 18.5%) were observed with ENS when expressed as g of CH4/kg of forage dry matter intake (fDMI) (P = 0.037). Lower concentrations of NH3-N and a higher ruminal pH were observed in ENS groups 6 h after supplementation (P < 0.05). Total VFA rumen concentration 6 h (P = 0.009) and 12 h after supplementation with EN resulted in lower acetate concentrations in the rumen (P = 0.041). Steers supplemented with EN had a greater ruminal abundance of Bacteroides, Barnesiella, Lactobacillus, Selenomonas, Veillonella, Succinimonas, Succinivibrio, and Duganella sp. (P < 0.05), but a lower abundance of Methanobrevibacter sp. (P = 0.007). Strong negative correlations were found between daily methane emissions and Proteobacteria, Erysipelotrichaceae, Prevotellaceae, and Roseburia, Kandleria, Selenomonas, Veillonella, and Succinivibrio sp. (P < 0.05) in the rumen of ENS steers. Encapsulated nitrate is a feed additive that persistently affects enteric methane emission in grazing steers, thereby decreasing Methanobrevibacter abundance in the rumen. In addition, ENS can promote fumarate-reducer and lactateproducer bacteria, thereby reducing acetate production during rumen fermentation. 2019-04-04T00:36:29Z 2019-04-04T00:36:29Z 2019-04-03 2019 2019-11-20T11:11:11Z Artigo de periódico Frontiers in microbiology, v. 10, n. 614, p. 1-12, 2019. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1107883 en eng openAccess |
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Archaea diversity Enteric methane emission Gado de Corte Nitrato Beef cattle Rumen bacteria Fatty acids Archaea diversity Enteric methane emission Gado de Corte Nitrato Beef cattle Rumen bacteria Fatty acids |
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Archaea diversity Enteric methane emission Gado de Corte Nitrato Beef cattle Rumen bacteria Fatty acids Archaea diversity Enteric methane emission Gado de Corte Nitrato Beef cattle Rumen bacteria Fatty acids GRANJA-SALCEDO, Y. T. FERNANDES, R. M. ARAUJO, R. C. de KISHI, L. T. BERCHIELLI, T. T. RESENDE, F. D. de BERNDT, A. SIQUEIRA, G. R. Long-Term encapsulated nitrate supplementation modulates rumen microbial diversity and rumen fermentation to reduce methane emission in grazing steers. |
description |
This study investigated the long-term effects (13 months) of encapsulated nitrate supplementation (ENS) on enteric methane emissions, rumen fermentation parameters, ruminal bacteria, and diversity of archaea in grazing beef cattle. We used a total of thirty-two Nellore steers (initial BW of 197 15.3 kg), 12 of which were fitted with rumen cannulas. For 13 months, the animals were maintained in 12 paddocks and fed a concentrate of ground corn, soybean meals, mineral supplements, and urea (URS) or encapsulated nitrate (EN) containing 70 g of EN/100 kg of BW (corresponding to 47 g NO3/100 kg BW). Encapsulated nitrate supplementation resulted in similar forage, supplement and total DMI values as URS (P > 0.05), but ENS tended to increase (C48 g/d; P = 0.055) average daily weight gain. Daily reductions in methane emissions (- 9.54 g or 18.5%) were observed with ENS when expressed as g of CH4/kg of forage dry matter intake (fDMI) (P = 0.037). Lower concentrations of NH3-N and a higher ruminal pH were observed in ENS groups 6 h after supplementation (P < 0.05). Total VFA rumen concentration 6 h (P = 0.009) and 12 h after supplementation with EN resulted in lower acetate concentrations in the rumen (P = 0.041). Steers supplemented with EN had a greater ruminal abundance of Bacteroides, Barnesiella, Lactobacillus, Selenomonas, Veillonella, Succinimonas, Succinivibrio, and Duganella sp. (P < 0.05), but a lower abundance of Methanobrevibacter sp. (P = 0.007). Strong negative correlations were found between daily methane emissions and Proteobacteria, Erysipelotrichaceae, Prevotellaceae, and Roseburia, Kandleria, Selenomonas, Veillonella, and Succinivibrio sp. (P < 0.05) in the rumen of ENS steers. Encapsulated nitrate is a feed additive that persistently affects enteric methane emission in grazing steers, thereby decreasing Methanobrevibacter abundance in the rumen. In addition, ENS can promote fumarate-reducer and lactateproducer bacteria, thereby reducing acetate production during rumen fermentation. |
author2 |
Yury Tatiana Granja-Salcedo, UNESP; Rodolfo Maciel Fernandes, UNESP/Agência Paulista de Tecnologia dos Agronegócios; Rafael Canonenco de Araujo, GRASP; Luciano Takeshi Kishi, UNESP; Telma Teresinha Berchielli, UNESP; Flávio Dutra de Resende, UNESP; ALEXANDRE BERNDT, CPPSE; Gustavo Rezende Siqueira, UNESP/Agência Paulista de Tecnologia dos Agronegócios. |
author_facet |
Yury Tatiana Granja-Salcedo, UNESP; Rodolfo Maciel Fernandes, UNESP/Agência Paulista de Tecnologia dos Agronegócios; Rafael Canonenco de Araujo, GRASP; Luciano Takeshi Kishi, UNESP; Telma Teresinha Berchielli, UNESP; Flávio Dutra de Resende, UNESP; ALEXANDRE BERNDT, CPPSE; Gustavo Rezende Siqueira, UNESP/Agência Paulista de Tecnologia dos Agronegócios. GRANJA-SALCEDO, Y. T. FERNANDES, R. M. ARAUJO, R. C. de KISHI, L. T. BERCHIELLI, T. T. RESENDE, F. D. de BERNDT, A. SIQUEIRA, G. R. |
format |
Artigo de periódico |
topic_facet |
Archaea diversity Enteric methane emission Gado de Corte Nitrato Beef cattle Rumen bacteria Fatty acids |
author |
GRANJA-SALCEDO, Y. T. FERNANDES, R. M. ARAUJO, R. C. de KISHI, L. T. BERCHIELLI, T. T. RESENDE, F. D. de BERNDT, A. SIQUEIRA, G. R. |
author_sort |
GRANJA-SALCEDO, Y. T. |
title |
Long-Term encapsulated nitrate supplementation modulates rumen microbial diversity and rumen fermentation to reduce methane emission in grazing steers. |
title_short |
Long-Term encapsulated nitrate supplementation modulates rumen microbial diversity and rumen fermentation to reduce methane emission in grazing steers. |
title_full |
Long-Term encapsulated nitrate supplementation modulates rumen microbial diversity and rumen fermentation to reduce methane emission in grazing steers. |
title_fullStr |
Long-Term encapsulated nitrate supplementation modulates rumen microbial diversity and rumen fermentation to reduce methane emission in grazing steers. |
title_full_unstemmed |
Long-Term encapsulated nitrate supplementation modulates rumen microbial diversity and rumen fermentation to reduce methane emission in grazing steers. |
title_sort |
long-term encapsulated nitrate supplementation modulates rumen microbial diversity and rumen fermentation to reduce methane emission in grazing steers. |
publishDate |
2019-04-03 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1107883 |
work_keys_str_mv |
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