Expression of paraoxonase types 1, 2 and 3 in reproductive tissues and activity of paraoxonase type 1 in the serum and seminal plasma of bulls.
The paraoxonases types 1, 2 and 3 (PON1, PON2 and PON3, respectively) are enzymes that degrade lipid peroxides, preventing oxidative damages relevant for male reproductive function. This study determined the expression of those three paraoxonases in reproductive tissues of bulls and evaluated correlations among the activity of PON1 in the serum and seminal plasma with breeding soundness parameters in bulls. The expression of PON1, PON2 and PON3 was characterised by RT-PCR in samples of testicular parenchyma, vesicular glands and epididymis collected from three slaughtered bulls. All three paraoxonases were expressed in the testicular parenchyma, PON2 and PON3 were both expressed in the epididymis head and PON3 was also expressed in the epididymis tail. The PON1 activity was determined in samples of serum and seminal plasma from 110 bulls submitted to breeding soundness evaluation. There was a strong correlation (r = .90) between the activity of the PON1 in both serum and seminal plasma (p < .0001). The PON1 activity in the seminal plasma was positively correlated with ejaculate?s colour, sperm mass activity (p = .04), motility, vigour and viability (all p < .01). Thus, PON1 may be a potential marker for sperm motility and viability in bulls.
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Format: | Separatas biblioteca |
Language: | English eng |
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2019-01-08
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Subjects: | Touro, Reprodução Animal, Esperma, Tecido Animal, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1103282 |
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dig-alice-doc-11032822019-01-09T00:25:58Z Expression of paraoxonase types 1, 2 and 3 in reproductive tissues and activity of paraoxonase type 1 in the serum and seminal plasma of bulls. FERREIRA, C. E. R. HAAS, C. S. GOULARTE, K. L. ROVANI, M. T. CARDOSO, F. F. SCHNEIDER, A. GASPERIN, B. G. LUCIA JUNIOR, T. C. E. R. Ferreira, UFPEL; C. S. Haas, UFPEL; K. L. Goularte, UFPEL; M. T. Rovani, UFPEL; FERNANDO FLORES CARDOSO, CPPSUL; A. Schneider, UFPEL; B. G. Gasperin, UFPEL; T. Lucia Junior, UFPEL. Touro Reprodução Animal Esperma Tecido Animal The paraoxonases types 1, 2 and 3 (PON1, PON2 and PON3, respectively) are enzymes that degrade lipid peroxides, preventing oxidative damages relevant for male reproductive function. This study determined the expression of those three paraoxonases in reproductive tissues of bulls and evaluated correlations among the activity of PON1 in the serum and seminal plasma with breeding soundness parameters in bulls. The expression of PON1, PON2 and PON3 was characterised by RT-PCR in samples of testicular parenchyma, vesicular glands and epididymis collected from three slaughtered bulls. All three paraoxonases were expressed in the testicular parenchyma, PON2 and PON3 were both expressed in the epididymis head and PON3 was also expressed in the epididymis tail. The PON1 activity was determined in samples of serum and seminal plasma from 110 bulls submitted to breeding soundness evaluation. There was a strong correlation (r = .90) between the activity of the PON1 in both serum and seminal plasma (p < .0001). The PON1 activity in the seminal plasma was positively correlated with ejaculate?s colour, sperm mass activity (p = .04), motility, vigour and viability (all p < .01). Thus, PON1 may be a potential marker for sperm motility and viability in bulls. 2019-01-09T00:25:52Z 2019-01-09T00:25:52Z 2019-01-08 2018 2019-01-09T00:25:52Z Separatas Andrologia, v. 50, n. 3, e12923, Apr. 2018. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1103282 en eng openAccess |
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Touro Reprodução Animal Esperma Tecido Animal Touro Reprodução Animal Esperma Tecido Animal |
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Touro Reprodução Animal Esperma Tecido Animal Touro Reprodução Animal Esperma Tecido Animal FERREIRA, C. E. R. HAAS, C. S. GOULARTE, K. L. ROVANI, M. T. CARDOSO, F. F. SCHNEIDER, A. GASPERIN, B. G. LUCIA JUNIOR, T. Expression of paraoxonase types 1, 2 and 3 in reproductive tissues and activity of paraoxonase type 1 in the serum and seminal plasma of bulls. |
description |
The paraoxonases types 1, 2 and 3 (PON1, PON2 and PON3, respectively) are enzymes that degrade lipid peroxides, preventing oxidative damages relevant for male reproductive function. This study determined the expression of those three paraoxonases in reproductive tissues of bulls and evaluated correlations among the activity of PON1 in the serum and seminal plasma with breeding soundness parameters in bulls. The expression of PON1, PON2 and PON3 was characterised by RT-PCR in samples of testicular parenchyma, vesicular glands and epididymis collected from three slaughtered bulls. All three paraoxonases were expressed in the testicular parenchyma, PON2 and PON3 were both expressed in the epididymis head and PON3 was also expressed in the epididymis tail. The PON1 activity was determined in samples of serum and seminal plasma from 110 bulls submitted to breeding soundness evaluation. There was a strong correlation (r = .90) between the activity of the PON1 in both serum and seminal plasma (p < .0001). The PON1 activity in the seminal plasma was positively correlated with ejaculate?s colour, sperm mass activity (p = .04), motility, vigour and viability (all p < .01). Thus, PON1 may be a potential marker for sperm motility and viability in bulls. |
author2 |
C. E. R. Ferreira, UFPEL; C. S. Haas, UFPEL; K. L. Goularte, UFPEL; M. T. Rovani, UFPEL; FERNANDO FLORES CARDOSO, CPPSUL; A. Schneider, UFPEL; B. G. Gasperin, UFPEL; T. Lucia Junior, UFPEL. |
author_facet |
C. E. R. Ferreira, UFPEL; C. S. Haas, UFPEL; K. L. Goularte, UFPEL; M. T. Rovani, UFPEL; FERNANDO FLORES CARDOSO, CPPSUL; A. Schneider, UFPEL; B. G. Gasperin, UFPEL; T. Lucia Junior, UFPEL. FERREIRA, C. E. R. HAAS, C. S. GOULARTE, K. L. ROVANI, M. T. CARDOSO, F. F. SCHNEIDER, A. GASPERIN, B. G. LUCIA JUNIOR, T. |
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Separatas |
topic_facet |
Touro Reprodução Animal Esperma Tecido Animal |
author |
FERREIRA, C. E. R. HAAS, C. S. GOULARTE, K. L. ROVANI, M. T. CARDOSO, F. F. SCHNEIDER, A. GASPERIN, B. G. LUCIA JUNIOR, T. |
author_sort |
FERREIRA, C. E. R. |
title |
Expression of paraoxonase types 1, 2 and 3 in reproductive tissues and activity of paraoxonase type 1 in the serum and seminal plasma of bulls. |
title_short |
Expression of paraoxonase types 1, 2 and 3 in reproductive tissues and activity of paraoxonase type 1 in the serum and seminal plasma of bulls. |
title_full |
Expression of paraoxonase types 1, 2 and 3 in reproductive tissues and activity of paraoxonase type 1 in the serum and seminal plasma of bulls. |
title_fullStr |
Expression of paraoxonase types 1, 2 and 3 in reproductive tissues and activity of paraoxonase type 1 in the serum and seminal plasma of bulls. |
title_full_unstemmed |
Expression of paraoxonase types 1, 2 and 3 in reproductive tissues and activity of paraoxonase type 1 in the serum and seminal plasma of bulls. |
title_sort |
expression of paraoxonase types 1, 2 and 3 in reproductive tissues and activity of paraoxonase type 1 in the serum and seminal plasma of bulls. |
publishDate |
2019-01-08 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1103282 |
work_keys_str_mv |
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