Relevance of endo-ß-mannanase enzyme in coffee seed deterioration process.
The endo-?-mannanase acts on the hemicellulose fraction of the endosperm cell walls, mainly mannans and galactomannans. This process weakens cell walls and allows radicle protrusion during seed germination, but may also occur during the deterioration process. Thus, the aim of this research was to determine the activity of endo-?-mannanase enzyme in dry coffee seeds and in soaked seeds, evaluating its relationship between physiological qualities. Coffee seeds obtained by different processing methods (natural, fermented and demucilated) and drying (sun, shade and dryer) were used. Seed quality was evaluated by germination and tetrazolium tests, and the endo-?-mannanase enzyme activity was determined in dry seeds and after 10 days of soaking. From the results, it was concluded that there is significant inverse relationship between the physiological quality of coffee seeds and the expression of endo-?-mannanase, and seeds with lower percentages of germination and viability of embryos have a higher activity of the enzyme. After ten days of soaking, coffee seeds had higher expression of endo-?-mannanase as compared to the dry seeds for all treatments of fruit processing and drying.
Main Authors: | FREITAS, M. N. de, ROSA, S. D. V. F. da, CLEMENTE, A. da C. S., PEREIRA, C. C. |
---|---|
Other Authors: | MARCELLA NUNES DE FREITAS, ESALQ; STTELA DELLYZETE VEIGA F DA ROSA, SAPC; ALINE DA CONSOLAÇÃO SAMPAIO CLEMENTE, UFLA; CRISTIANE CARVALHO PEREIRA, UFLA. |
Format: | Artigo de periódico biblioteca |
Language: | English eng |
Published: |
2017-12-08
|
Subjects: | Physiological quality, Processing, Drying, Hemicellulose, Endosperm, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1081994 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Insights into the xylan degradation system of Cellulomonas sp. B6 : biochemical characterization of rCsXyn10A and rCsAbf62A
by: Garrido, Mercedes Maria, et al.
Published: (2022) -
Cell wall compounds in the meal of macaúba from Pantanal.
by: ALMEIDA, M. A, et al.
Published: (2012-10-24) -
Cellulose nanocrystal-based films produced by more sustainable extraction protocols from Posidonia oceanica waste biomass
by: Benito González, Isaac, et al.
Published: (2019-07-22) -
Lignocellulosic nanofibers from triticale straw : the influence of hemicelluloses and lignin in their production and properties
by: Tarrés, Quim, et al. -
Puritication and structural characterization of a (4-O-methyl)-glucuronoxylan from gabiroba fruit (campomanesia xanthocarpa berg).
by: BARBIERI, S. F., et al.
Published: (2017-09-21)