Relevance of endo-ß-mannanase enzyme in coffee seed deterioration process.
The endo-?-mannanase acts on the hemicellulose fraction of the endosperm cell walls, mainly mannans and galactomannans. This process weakens cell walls and allows radicle protrusion during seed germination, but may also occur during the deterioration process. Thus, the aim of this research was to determine the activity of endo-?-mannanase enzyme in dry coffee seeds and in soaked seeds, evaluating its relationship between physiological qualities. Coffee seeds obtained by different processing methods (natural, fermented and demucilated) and drying (sun, shade and dryer) were used. Seed quality was evaluated by germination and tetrazolium tests, and the endo-?-mannanase enzyme activity was determined in dry seeds and after 10 days of soaking. From the results, it was concluded that there is significant inverse relationship between the physiological quality of coffee seeds and the expression of endo-?-mannanase, and seeds with lower percentages of germination and viability of embryos have a higher activity of the enzyme. After ten days of soaking, coffee seeds had higher expression of endo-?-mannanase as compared to the dry seeds for all treatments of fruit processing and drying.
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Format: | Artigo de periódico biblioteca |
Language: | English eng |
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2017-12-08
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Subjects: | Physiological quality, Processing, Drying, Hemicellulose, Endosperm, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1081994 |
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dig-alice-doc-10819942017-12-08T23:28:05Z Relevance of endo-ß-mannanase enzyme in coffee seed deterioration process. FREITAS, M. N. de ROSA, S. D. V. F. da CLEMENTE, A. da C. S. PEREIRA, C. C. MARCELLA NUNES DE FREITAS, ESALQ; STTELA DELLYZETE VEIGA F DA ROSA, SAPC; ALINE DA CONSOLAÇÃO SAMPAIO CLEMENTE, UFLA; CRISTIANE CARVALHO PEREIRA, UFLA. Physiological quality Processing Drying Hemicellulose Endosperm The endo-?-mannanase acts on the hemicellulose fraction of the endosperm cell walls, mainly mannans and galactomannans. This process weakens cell walls and allows radicle protrusion during seed germination, but may also occur during the deterioration process. Thus, the aim of this research was to determine the activity of endo-?-mannanase enzyme in dry coffee seeds and in soaked seeds, evaluating its relationship between physiological qualities. Coffee seeds obtained by different processing methods (natural, fermented and demucilated) and drying (sun, shade and dryer) were used. Seed quality was evaluated by germination and tetrazolium tests, and the endo-?-mannanase enzyme activity was determined in dry seeds and after 10 days of soaking. From the results, it was concluded that there is significant inverse relationship between the physiological quality of coffee seeds and the expression of endo-?-mannanase, and seeds with lower percentages of germination and viability of embryos have a higher activity of the enzyme. After ten days of soaking, coffee seeds had higher expression of endo-?-mannanase as compared to the dry seeds for all treatments of fruit processing and drying. 2017-12-08T23:27:58Z 2017-12-08T23:27:58Z 2017-12-08 2017 2019-05-09T11:11:11Z Artigo de periódico African Journal of Agricultural Research, v. 12, n. 15, p. 1253-1258, April, 2017 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1081994 en eng openAccess |
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Physiological quality Processing Drying Hemicellulose Endosperm Physiological quality Processing Drying Hemicellulose Endosperm |
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Physiological quality Processing Drying Hemicellulose Endosperm Physiological quality Processing Drying Hemicellulose Endosperm FREITAS, M. N. de ROSA, S. D. V. F. da CLEMENTE, A. da C. S. PEREIRA, C. C. Relevance of endo-ß-mannanase enzyme in coffee seed deterioration process. |
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The endo-?-mannanase acts on the hemicellulose fraction of the endosperm cell walls, mainly mannans and galactomannans. This process weakens cell walls and allows radicle protrusion during seed germination, but may also occur during the deterioration process. Thus, the aim of this research was to determine the activity of endo-?-mannanase enzyme in dry coffee seeds and in soaked seeds, evaluating its relationship between physiological qualities. Coffee seeds obtained by different processing methods (natural, fermented and demucilated) and drying (sun, shade and dryer) were used. Seed quality was evaluated by germination and tetrazolium tests, and the endo-?-mannanase enzyme activity was determined in dry seeds and after 10 days of soaking. From the results, it was concluded that there is significant inverse relationship between the physiological quality of coffee seeds and the expression of endo-?-mannanase, and seeds with lower percentages of germination and viability of embryos have a higher activity of the enzyme. After ten days of soaking, coffee seeds had higher expression of endo-?-mannanase as compared to the dry seeds for all treatments of fruit processing and drying. |
author2 |
MARCELLA NUNES DE FREITAS, ESALQ; STTELA DELLYZETE VEIGA F DA ROSA, SAPC; ALINE DA CONSOLAÇÃO SAMPAIO CLEMENTE, UFLA; CRISTIANE CARVALHO PEREIRA, UFLA. |
author_facet |
MARCELLA NUNES DE FREITAS, ESALQ; STTELA DELLYZETE VEIGA F DA ROSA, SAPC; ALINE DA CONSOLAÇÃO SAMPAIO CLEMENTE, UFLA; CRISTIANE CARVALHO PEREIRA, UFLA. FREITAS, M. N. de ROSA, S. D. V. F. da CLEMENTE, A. da C. S. PEREIRA, C. C. |
format |
Artigo de periódico |
topic_facet |
Physiological quality Processing Drying Hemicellulose Endosperm |
author |
FREITAS, M. N. de ROSA, S. D. V. F. da CLEMENTE, A. da C. S. PEREIRA, C. C. |
author_sort |
FREITAS, M. N. de |
title |
Relevance of endo-ß-mannanase enzyme in coffee seed deterioration process. |
title_short |
Relevance of endo-ß-mannanase enzyme in coffee seed deterioration process. |
title_full |
Relevance of endo-ß-mannanase enzyme in coffee seed deterioration process. |
title_fullStr |
Relevance of endo-ß-mannanase enzyme in coffee seed deterioration process. |
title_full_unstemmed |
Relevance of endo-ß-mannanase enzyme in coffee seed deterioration process. |
title_sort |
relevance of endo-ß-mannanase enzyme in coffee seed deterioration process. |
publishDate |
2017-12-08 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1081994 |
work_keys_str_mv |
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