Determination of selenium in bovine semen by ICP-MS using formic acid for sample preparation.
Determination of Se by inductively coupled plasma mass spectrometry (ICP-MS) can be difficult due to spectral interferences; however, these interferences can be eliminated by using collision-reaction interface (CRI) technology. A simple and fast procedure for determination of total Se concentration in bovine semen by ICP-MS after sample solubilization with diluted formic acid is here proposed. Formic acid caused an increase of up to 50% in selenium net signals and the maximum gain was obtained at 10% v v-1 formic acid solution. The optimized nebulizer gas flow rates were 1.05 and 0.95 L min-1, and radio frequency applied power were 1.3 and 1.4 kW without and with CRI, respectively. Microwave-assisted acid digestion was also implemented in the preparation of semen sample for comparison of results. No statistical differences between these sample preparation strategies at 95% confidence level (t-test) were observed for 76Se+ , 77Se+ , 78Se+ and 82Se+ without using CRI and 82Se+ with CRI.
Main Authors: | BIANCHI, S. R., AMARAL, C. D. B. do, SILVA, C. S. da, NOGUEIRA, A. R. de A. |
---|---|
Other Authors: | SILMARA ROSSANA BIANCHI, CNPS; Clarice Dias Britto do Amaral, UFPR; Caroline Santos da Silva, UFSCar; ANA RITA DE ARAUJO NOGUEIRA, CPPSE. |
Format: | Artigo de periódico biblioteca |
Language: | English eng |
Published: |
2017-11-14
|
Subjects: | Bovine semen, Cattle fertility, selenium, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1079670 http://dx.doi.org/10.21577/0103-5053.20170089 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Determination of selenium in bovine semen by ICP-MS using formic acid for sample preparation.
by: BIANCHI, S. R., et al.
Published: (2021-09-21) -
Selenium concentration in cattle serum and fodder from two areas in Ethiopia with contrasting human selenium concentration
by: Hailu, K., et al.
Published: (2022) -
Organic vs. inorganic selenium in farm animal nutrition with special reference to supplementation of cattle
by: Ortman, K.
Published: (1999) -
Comparison between liquid and deep-frozen semen for artificial insemination in developing and developed countries
by: Schuh, H. -
The effect of supplementary selenium on performance of bulls fed high levels of molasses, Mauritius
by: FAO, Rome (Italy) eng
Published: (Jul )