Enhancement of Trichoderma harzianum CFAM-422 for cellulase and hemicellulase production by deletion of the carbon catabolite repressor gene cre1.
Carbon catabolite repression (CCR) is a mechanism by which microorganisms can utilize preferably highly energetic compounds over those of difficult degradation. For Trichoderma reesei, the protein that acts as repressor in the presence of glucose is CRE1. In this project, we aim to delete cre1 gene in Trichoderma harzianum CFAM-422 and obtain mutants with enhanced production of biomass degrading enzymes. Disruption of cre1 in T. harzianum CFAM-422 was performed by gene replacement of cre1 for hph (hygromycin B phosphotransferase) via homologous recombination. Hygromycin resistant mutants and parental strains enzyme production was evaluated in both inductive and repressive conditions in four different carbon sources. Enzymatic indexes (EI) were determined and compared. All genetically stable transformants showed increased enzymatic index under inductive conditions and modest inhibition under repressive conditions for most carbon sources, indicating that the deletion of cre1 in T. harzianum can be beneficial to cellulase and hemicellulase production with reduced product inhibition.
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| Format: | Separatas biblioteca |
| Language: | English eng |
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2017-10-09
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| Subjects: | Processos de hidrolise enzimática de biomassa, Carbon Catabolite Repression, Cellulolytic and hemicellulolytic enzymes., Celulase, Trichoderma Harzianum., |
| Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076994 |
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dig-alice-doc-10769942017-12-08T23:19:35Z Enhancement of Trichoderma harzianum CFAM-422 for cellulase and hemicellulase production by deletion of the carbon catabolite repressor gene cre1. TAMIETTI, M. S. MIDORIKAWA, G. E. O. MENDES, T. D. DAMASO, M. C. T. BOM, E. P. S. SILVA, A. S. GOTTSCHALK, L. M. F. NORONHA, E. F. FAVARO, L. C. de L. MARIANA S. TAMIETTI, UNB; GLÁUCIA E. O. MIDORIKAWA; THAIS DEMARCHI MENDES, CNPAE; MONICA CARAMEZ TRICHES DAMASO, CNPAE; ELBA P. S. BOM, UFRJ; AYLA S. SILVA, INT; LEDA MARIA FORTES GOTTSCHALK, CTAA; ELIANE F. NORONHA, UNB; LEIA CECILIA DE LIMA FAVARO, CNPAE. Processos de hidrolise enzimática de biomassa Carbon Catabolite Repression Cellulolytic and hemicellulolytic enzymes. Celulase Trichoderma Harzianum. Carbon catabolite repression (CCR) is a mechanism by which microorganisms can utilize preferably highly energetic compounds over those of difficult degradation. For Trichoderma reesei, the protein that acts as repressor in the presence of glucose is CRE1. In this project, we aim to delete cre1 gene in Trichoderma harzianum CFAM-422 and obtain mutants with enhanced production of biomass degrading enzymes. Disruption of cre1 in T. harzianum CFAM-422 was performed by gene replacement of cre1 for hph (hygromycin B phosphotransferase) via homologous recombination. Hygromycin resistant mutants and parental strains enzyme production was evaluated in both inductive and repressive conditions in four different carbon sources. Enzymatic indexes (EI) were determined and compared. All genetically stable transformants showed increased enzymatic index under inductive conditions and modest inhibition under repressive conditions for most carbon sources, indicating that the deletion of cre1 in T. harzianum can be beneficial to cellulase and hemicellulase production with reduced product inhibition. SINAFERM; SHEB. 3 a 6 de setembro. Seção Trabalhos. Ref. 59019. 2017-12-08T23:19:28Z 2017-12-08T23:19:28Z 2017-10-09 2017 2017-12-08T23:19:28Z Separatas In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 21.; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 12., 2017, Aracaju, SE. [Proceedings ...]. São Paulo: Associação Brasileira de Engenharia Química, 2017. 2447-2816 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076994 en eng openAccess Não paginado. |
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Processos de hidrolise enzimática de biomassa Carbon Catabolite Repression Cellulolytic and hemicellulolytic enzymes. Celulase Trichoderma Harzianum. Processos de hidrolise enzimática de biomassa Carbon Catabolite Repression Cellulolytic and hemicellulolytic enzymes. Celulase Trichoderma Harzianum. |
| spellingShingle |
Processos de hidrolise enzimática de biomassa Carbon Catabolite Repression Cellulolytic and hemicellulolytic enzymes. Celulase Trichoderma Harzianum. Processos de hidrolise enzimática de biomassa Carbon Catabolite Repression Cellulolytic and hemicellulolytic enzymes. Celulase Trichoderma Harzianum. TAMIETTI, M. S. MIDORIKAWA, G. E. O. MENDES, T. D. DAMASO, M. C. T. BOM, E. P. S. SILVA, A. S. GOTTSCHALK, L. M. F. NORONHA, E. F. FAVARO, L. C. de L. Enhancement of Trichoderma harzianum CFAM-422 for cellulase and hemicellulase production by deletion of the carbon catabolite repressor gene cre1. |
| description |
Carbon catabolite repression (CCR) is a mechanism by which microorganisms can utilize preferably highly energetic compounds over those of difficult degradation. For Trichoderma reesei, the protein that acts as repressor in the presence of glucose is CRE1. In this project, we aim to delete cre1 gene in Trichoderma harzianum CFAM-422 and obtain mutants with enhanced production of biomass degrading enzymes. Disruption of cre1 in T. harzianum CFAM-422 was performed by gene replacement of cre1 for hph (hygromycin B phosphotransferase) via homologous recombination. Hygromycin resistant mutants and parental strains enzyme production was evaluated in both inductive and repressive conditions in four different carbon sources. Enzymatic indexes (EI) were determined and compared. All genetically stable transformants showed increased enzymatic index under inductive conditions and modest inhibition under repressive conditions for most carbon sources, indicating that the deletion of cre1 in T. harzianum can be beneficial to cellulase and hemicellulase production with reduced product inhibition. |
| author2 |
MARIANA S. TAMIETTI, UNB; GLÁUCIA E. O. MIDORIKAWA; THAIS DEMARCHI MENDES, CNPAE; MONICA CARAMEZ TRICHES DAMASO, CNPAE; ELBA P. S. BOM, UFRJ; AYLA S. SILVA, INT; LEDA MARIA FORTES GOTTSCHALK, CTAA; ELIANE F. NORONHA, UNB; LEIA CECILIA DE LIMA FAVARO, CNPAE. |
| author_facet |
MARIANA S. TAMIETTI, UNB; GLÁUCIA E. O. MIDORIKAWA; THAIS DEMARCHI MENDES, CNPAE; MONICA CARAMEZ TRICHES DAMASO, CNPAE; ELBA P. S. BOM, UFRJ; AYLA S. SILVA, INT; LEDA MARIA FORTES GOTTSCHALK, CTAA; ELIANE F. NORONHA, UNB; LEIA CECILIA DE LIMA FAVARO, CNPAE. TAMIETTI, M. S. MIDORIKAWA, G. E. O. MENDES, T. D. DAMASO, M. C. T. BOM, E. P. S. SILVA, A. S. GOTTSCHALK, L. M. F. NORONHA, E. F. FAVARO, L. C. de L. |
| format |
Separatas |
| topic_facet |
Processos de hidrolise enzimática de biomassa Carbon Catabolite Repression Cellulolytic and hemicellulolytic enzymes. Celulase Trichoderma Harzianum. |
| author |
TAMIETTI, M. S. MIDORIKAWA, G. E. O. MENDES, T. D. DAMASO, M. C. T. BOM, E. P. S. SILVA, A. S. GOTTSCHALK, L. M. F. NORONHA, E. F. FAVARO, L. C. de L. |
| author_sort |
TAMIETTI, M. S. |
| title |
Enhancement of Trichoderma harzianum CFAM-422 for cellulase and hemicellulase production by deletion of the carbon catabolite repressor gene cre1. |
| title_short |
Enhancement of Trichoderma harzianum CFAM-422 for cellulase and hemicellulase production by deletion of the carbon catabolite repressor gene cre1. |
| title_full |
Enhancement of Trichoderma harzianum CFAM-422 for cellulase and hemicellulase production by deletion of the carbon catabolite repressor gene cre1. |
| title_fullStr |
Enhancement of Trichoderma harzianum CFAM-422 for cellulase and hemicellulase production by deletion of the carbon catabolite repressor gene cre1. |
| title_full_unstemmed |
Enhancement of Trichoderma harzianum CFAM-422 for cellulase and hemicellulase production by deletion of the carbon catabolite repressor gene cre1. |
| title_sort |
enhancement of trichoderma harzianum cfam-422 for cellulase and hemicellulase production by deletion of the carbon catabolite repressor gene cre1. |
| publishDate |
2017-10-09 |
| url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076994 |
| work_keys_str_mv |
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