RNA-seq analysis of the Rhizobium tropici CIAT 899 Transcripitome shows similarites in the activation patterns of symbiotic genes in the presence of apigenin and salt.
Rhizobium tropici strain CIAT 899 establishes effective symbioses with several legume species, including Phaseolus vulgaris and Leucaena leucocephala. This bacterium synthesizes a large variety of nodulation factors in response to nod-gene inducing flavonoids and, surprisingly, also under salt stress conditions. The aim of this study was to identify differentially expressed genes in the presence of both inducer molecules, and analyze the promoter regions located upstream of these genes. Results obtained by RNA-seq analyses of CIAT 899 induced with apigenin, a nod gene-inducing flavonoid for this strain, or salt allowed the identification of 19 and 790 differentially expressed genes, respectively. Fifteen of these genes were up-regulated in both conditions and were involved in the synthesis of both Nod factors and indole-3-acetic acid. Transcription of these genes was presumably activated through binding of at least one of the five NodD proteins present in this strain to specific nod box promoter sequences when the bacterium was induced by both apigenin and salt. Finally, under saline conditions, many other transcriptional responses were detected, including an increase in the transcription of genes involved in trehalose catabolism, chemotaxis and protein secretion, as well as ribosomal genes, and a decrease in the transcription of genes involved in transmembrane transport. To our knowledge this is the first time that a transcriptomic study shows that salt stress induces the expression of nodulation genes in the absence of flavonoids. Thus, in the presence of both nodulation inducer molecules, apigenin and salt, R. tropici CIAT 899 up-regulated the same set of symbiotic genes. It could be possible that the increases in the transcription levels of several genes related to nodulation under saline conditions could represent a strategy to establish symbiosis under abiotic stressing conditions.
Main Authors: | , , , , , , , |
---|---|
Other Authors: | |
Format: | Separatas biblioteca |
Language: | English eng |
Published: |
2016-03-22
|
Subjects: | RNA-seq, Nod factors, Lipochitooligosaccharides, Nodutlation., Nodulação, Rhizobium, Rhizobium tropici, Apigenin, Salt stress., |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1041600 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-alice-doc-1041600 |
---|---|
record_format |
koha |
spelling |
dig-alice-doc-10416002017-08-16T03:28:27Z RNA-seq analysis of the Rhizobium tropici CIAT 899 Transcripitome shows similarites in the activation patterns of symbiotic genes in the presence of apigenin and salt. PÉREZ-MONTAÑO, F. CERRO, P. del JIMÉNEZ-GUERRERO, I. LÓPEZ-BAENA, F. J. CUBO, M. T. HUNGRIA, M. MEGÍAS, M. OLLERO, F. J. Francisco Péres-Montaño, Universidade de Sevilla; Pablo del Cerro, Universidade de Sevilia; Irene Jiménez-Guerreiro, Universidade de Sevilla; Francisco Javier López-Baena, Universidade de Sevilla; maria Teresa Cubo, Universidade de Sevilla; MARIANGELA HUNGRIA DA CUNHA, CNPSO; Manuel Megías, Universidade de Sevilla; Francisco Javier Ollero, Uiversidade de Sevilla. RNA-seq Nod factors Lipochitooligosaccharides Nodutlation. Nodulação Rhizobium Rhizobium tropici Apigenin Salt stress. Rhizobium tropici strain CIAT 899 establishes effective symbioses with several legume species, including Phaseolus vulgaris and Leucaena leucocephala. This bacterium synthesizes a large variety of nodulation factors in response to nod-gene inducing flavonoids and, surprisingly, also under salt stress conditions. The aim of this study was to identify differentially expressed genes in the presence of both inducer molecules, and analyze the promoter regions located upstream of these genes. Results obtained by RNA-seq analyses of CIAT 899 induced with apigenin, a nod gene-inducing flavonoid for this strain, or salt allowed the identification of 19 and 790 differentially expressed genes, respectively. Fifteen of these genes were up-regulated in both conditions and were involved in the synthesis of both Nod factors and indole-3-acetic acid. Transcription of these genes was presumably activated through binding of at least one of the five NodD proteins present in this strain to specific nod box promoter sequences when the bacterium was induced by both apigenin and salt. Finally, under saline conditions, many other transcriptional responses were detected, including an increase in the transcription of genes involved in trehalose catabolism, chemotaxis and protein secretion, as well as ribosomal genes, and a decrease in the transcription of genes involved in transmembrane transport. To our knowledge this is the first time that a transcriptomic study shows that salt stress induces the expression of nodulation genes in the absence of flavonoids. Thus, in the presence of both nodulation inducer molecules, apigenin and salt, R. tropici CIAT 899 up-regulated the same set of symbiotic genes. It could be possible that the increases in the transcription levels of several genes related to nodulation under saline conditions could represent a strategy to establish symbiosis under abiotic stressing conditions. 2016-03-22T11:11:11Z 2016-03-22T11:11:11Z 2016-03-22 2016 2017-06-19T11:11:11Z Separatas BMC Genomics, v. 17, n. 1, p. 198, Mar. 2016. 1471-2164 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1041600 10.1186/s12864-016-2543-3 en eng openAccess |
institution |
EMBRAPA |
collection |
DSpace |
country |
Brasil |
countrycode |
BR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-alice |
tag |
biblioteca |
region |
America del Sur |
libraryname |
Sistema de bibliotecas de EMBRAPA |
language |
English eng |
topic |
RNA-seq Nod factors Lipochitooligosaccharides Nodutlation. Nodulação Rhizobium Rhizobium tropici Apigenin Salt stress. RNA-seq Nod factors Lipochitooligosaccharides Nodutlation. Nodulação Rhizobium Rhizobium tropici Apigenin Salt stress. |
spellingShingle |
RNA-seq Nod factors Lipochitooligosaccharides Nodutlation. Nodulação Rhizobium Rhizobium tropici Apigenin Salt stress. RNA-seq Nod factors Lipochitooligosaccharides Nodutlation. Nodulação Rhizobium Rhizobium tropici Apigenin Salt stress. PÉREZ-MONTAÑO, F. CERRO, P. del JIMÉNEZ-GUERRERO, I. LÓPEZ-BAENA, F. J. CUBO, M. T. HUNGRIA, M. MEGÍAS, M. OLLERO, F. J. RNA-seq analysis of the Rhizobium tropici CIAT 899 Transcripitome shows similarites in the activation patterns of symbiotic genes in the presence of apigenin and salt. |
description |
Rhizobium tropici strain CIAT 899 establishes effective symbioses with several legume species, including Phaseolus vulgaris and Leucaena leucocephala. This bacterium synthesizes a large variety of nodulation factors in response to nod-gene inducing flavonoids and, surprisingly, also under salt stress conditions. The aim of this study was to identify differentially expressed genes in the presence of both inducer molecules, and analyze the promoter regions located upstream of these genes. Results obtained by RNA-seq analyses of CIAT 899 induced with apigenin, a nod gene-inducing flavonoid for this strain, or salt allowed the identification of 19 and 790 differentially expressed genes, respectively. Fifteen of these genes were up-regulated in both conditions and were involved in the synthesis of both Nod factors and indole-3-acetic acid. Transcription of these genes was presumably activated through binding of at least one of the five NodD proteins present in this strain to specific nod box promoter sequences when the bacterium was induced by both apigenin and salt. Finally, under saline conditions, many other transcriptional responses were detected, including an increase in the transcription of genes involved in trehalose catabolism, chemotaxis and protein secretion, as well as ribosomal genes, and a decrease in the transcription of genes involved in transmembrane transport. To our knowledge this is the first time that a transcriptomic study shows that salt stress induces the expression of nodulation genes in the absence of flavonoids. Thus, in the presence of both nodulation inducer molecules, apigenin and salt, R. tropici CIAT 899 up-regulated the same set of symbiotic genes. It could be possible that the increases in the transcription levels of several genes related to nodulation under saline conditions could represent a strategy to establish symbiosis under abiotic stressing conditions. |
author2 |
Francisco Péres-Montaño, Universidade de Sevilla; Pablo del Cerro, Universidade de Sevilia; Irene Jiménez-Guerreiro, Universidade de Sevilla; Francisco Javier López-Baena, Universidade de Sevilla; maria Teresa Cubo, Universidade de Sevilla; MARIANGELA HUNGRIA DA CUNHA, CNPSO; Manuel Megías, Universidade de Sevilla; Francisco Javier Ollero, Uiversidade de Sevilla. |
author_facet |
Francisco Péres-Montaño, Universidade de Sevilla; Pablo del Cerro, Universidade de Sevilia; Irene Jiménez-Guerreiro, Universidade de Sevilla; Francisco Javier López-Baena, Universidade de Sevilla; maria Teresa Cubo, Universidade de Sevilla; MARIANGELA HUNGRIA DA CUNHA, CNPSO; Manuel Megías, Universidade de Sevilla; Francisco Javier Ollero, Uiversidade de Sevilla. PÉREZ-MONTAÑO, F. CERRO, P. del JIMÉNEZ-GUERRERO, I. LÓPEZ-BAENA, F. J. CUBO, M. T. HUNGRIA, M. MEGÍAS, M. OLLERO, F. J. |
format |
Separatas |
topic_facet |
RNA-seq Nod factors Lipochitooligosaccharides Nodutlation. Nodulação Rhizobium Rhizobium tropici Apigenin Salt stress. |
author |
PÉREZ-MONTAÑO, F. CERRO, P. del JIMÉNEZ-GUERRERO, I. LÓPEZ-BAENA, F. J. CUBO, M. T. HUNGRIA, M. MEGÍAS, M. OLLERO, F. J. |
author_sort |
PÉREZ-MONTAÑO, F. |
title |
RNA-seq analysis of the Rhizobium tropici CIAT 899 Transcripitome shows similarites in the activation patterns of symbiotic genes in the presence of apigenin and salt. |
title_short |
RNA-seq analysis of the Rhizobium tropici CIAT 899 Transcripitome shows similarites in the activation patterns of symbiotic genes in the presence of apigenin and salt. |
title_full |
RNA-seq analysis of the Rhizobium tropici CIAT 899 Transcripitome shows similarites in the activation patterns of symbiotic genes in the presence of apigenin and salt. |
title_fullStr |
RNA-seq analysis of the Rhizobium tropici CIAT 899 Transcripitome shows similarites in the activation patterns of symbiotic genes in the presence of apigenin and salt. |
title_full_unstemmed |
RNA-seq analysis of the Rhizobium tropici CIAT 899 Transcripitome shows similarites in the activation patterns of symbiotic genes in the presence of apigenin and salt. |
title_sort |
rna-seq analysis of the rhizobium tropici ciat 899 transcripitome shows similarites in the activation patterns of symbiotic genes in the presence of apigenin and salt. |
publishDate |
2016-03-22 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1041600 |
work_keys_str_mv |
AT perezmontanof rnaseqanalysisoftherhizobiumtropiciciat899transcripitomeshowssimilaritesintheactivationpatternsofsymbioticgenesinthepresenceofapigeninandsalt AT cerropdel rnaseqanalysisoftherhizobiumtropiciciat899transcripitomeshowssimilaritesintheactivationpatternsofsymbioticgenesinthepresenceofapigeninandsalt AT jimenezguerreroi rnaseqanalysisoftherhizobiumtropiciciat899transcripitomeshowssimilaritesintheactivationpatternsofsymbioticgenesinthepresenceofapigeninandsalt AT lopezbaenafj rnaseqanalysisoftherhizobiumtropiciciat899transcripitomeshowssimilaritesintheactivationpatternsofsymbioticgenesinthepresenceofapigeninandsalt AT cubomt rnaseqanalysisoftherhizobiumtropiciciat899transcripitomeshowssimilaritesintheactivationpatternsofsymbioticgenesinthepresenceofapigeninandsalt AT hungriam rnaseqanalysisoftherhizobiumtropiciciat899transcripitomeshowssimilaritesintheactivationpatternsofsymbioticgenesinthepresenceofapigeninandsalt AT megiasm rnaseqanalysisoftherhizobiumtropiciciat899transcripitomeshowssimilaritesintheactivationpatternsofsymbioticgenesinthepresenceofapigeninandsalt AT ollerofj rnaseqanalysisoftherhizobiumtropiciciat899transcripitomeshowssimilaritesintheactivationpatternsofsymbioticgenesinthepresenceofapigeninandsalt |
_version_ |
1756022349278216192 |