Exopolysaccharide from Agaricus brasiliensis LPB and its Scale Up Studies in a Stirred Tank Fermenter.

Background and purpose: Although the activities of polysaccharides such as beta-glucans are well known in Agaricus brasiliensis, there has been little research on their exopolysaccharides and its characterization or scale up studies. The aim of the study was to study the monosaccharide composition of the exopolysaccharide (EPS) produced by Agaricus brasiliensis LPB3 and its production in a stirred tank fermenter. Materials and methods: Agaricus brasiliensis LPB3 was cultured in submerged fermentation in a medium containing glucose, yeast extract, K2HPO4 and MgSO4. Extracellular polysaccharide was precipitated with four volumes of 95% ethanol. For monosaccharide composition, the EPSs were hydrolyzed, derivatized to alditol acetates, and analyzed by gas chromatography (GC) and GC-mass spectrometry (GC-MS). Results and conclusions: The exopolysaccharide produced by Agaricus brasiliensis was predominantly composed by mannose (57,7%) and galactose (28,2%) residues. The studies in a stirred tank fermenter kept the productivity obtained in laboratory scale regarding the production of exopolysaccharides (≈ 1200 mg/L). This result can be considered promising since scale up approach can decrease its yield.

Bibliographic Details

Main Authors:	GERN, J. C., SANTOS, L. F. dos, HABU, S., NOSEDA, M. D., LIMA, L. F. O. de, SILVA, A. L. L. da, SOCCOL, V. T., BRANDAO, H. de M., SOCCOL, C. R.
Other Authors:	JULIANA CARINE GERN, CNPGL; LEANDRO FREIRE dos SANTOS, Universidade Federal do Paraná; SASCHA HABU, Universidade Federal do Paraná; USP; MIGUEL DANIEL NOSEDA, Universidade Federal do Paraná; LUCIANNA FREITAS OLIVEIRA de LIMA, Universidade Federal do Paraná; ANDRÉ LUÍS LOPES da SILVA, Universidade Federal do Paraná; VANETE THOMAZ SOCCOL, Universidade Federal do Paraná; HUMBERTO DE MELLO BRANDAO, CNPGL; CARLOS RICARDO SOCCOL, Universidade Federal do Paraná.
Format:	Separatas biblioteca
Language:	English eng
Published:	2016-02-19
Subjects:	Exopolysaccharide, Agaricus brasiliensis, Scale up, Monosaccharide composition, Stirred tank fermenter, Basidiomycete,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1037830
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