de novo genome assembly of the South American freshwater fish Tambaqui (Colossoma macropomum).
The Tambaqui (Colossoma macropomum) is a freshwater fish species naturally found in the Amazon river basin which has historically been widely exploited by community and commercial fishing. Recent efforts to domesticate, breed and raise the species in aquaculture systems has led to significant increases in production (>10-fold) over the last ten years. Current production is above 120,000 metric tons per year with a strong growth trend, making it the most important native aquaculture species in Brazil. Data for generating the draft assembly were produced from shotgun libraries with two different insert sizes and mate-paired libraries with four different sizes sequenced (2x150bps) with Illumina HiSeq2000 technology. A total of 124.8Gbp quality-filtered nucleotides were sequenced which amount to 85x mean genome coverage, considering previously published information (C-value = 1,5pg = 1.467Gbp). Sequence assembly was performed with SOAPdenovo and generated 8.924 scaffolds spanning 1.54 Gbp (N50: 2,041,733bp (162 scaffolds), N90: 200,945bp (1009 scaffolds), ~500Mbp of unmapped nucleotides). Gene model prediction is underway with MAKER2 using as extrinsic evidence protein and EST data from phylogenetically related taxa. This represents the first report of a draft genome sequence for this species and will be a valuable source of information for marker detection/selection, genetic improvement, conservation and basic biology studies in this species.
Main Authors: | , , , , , , , |
---|---|
Other Authors: | |
Format: | Anais e Proceedings de eventos biblioteca |
Language: | English eng |
Published: |
2015-04-10
|
Subjects: | Sequência genômica., Tambaqui, Colossoma Macropomum., Genome assembly, Sequence analysis., |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1013201 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-alice-doc-1013201 |
---|---|
record_format |
koha |
spelling |
dig-alice-doc-10132012017-08-16T03:33:47Z de novo genome assembly of the South American freshwater fish Tambaqui (Colossoma macropomum). LOBO, F. P. CINTRA, L. C. VARELA, E. S. ALVES, A. L. VILLELA, L. C. V. SILVA, N. M. A. da PAIVA, S. R. CAETANO, A. R. FRANCISCO PEREIRA LOBO, CNPTIA; LEANDRO CARRIJO CINTRA, CNPTIA; EDUARDO SOUSA VARELA, CNPASA; ANDERSON LUIS ALVES, CNPASA; LUCIANA CRISTINE VASQUES VILLELA, UnB; NAIARA MILAGRES AUGUSTO DA SILVA, CENARGEN; SAMUEL REZENDE PAIVA, SRI; ALEXANDRE RODRIGUES CAETANO, CENARGEN. Sequência genômica. Tambaqui Colossoma Macropomum. Genome assembly Sequence analysis. The Tambaqui (Colossoma macropomum) is a freshwater fish species naturally found in the Amazon river basin which has historically been widely exploited by community and commercial fishing. Recent efforts to domesticate, breed and raise the species in aquaculture systems has led to significant increases in production (>10-fold) over the last ten years. Current production is above 120,000 metric tons per year with a strong growth trend, making it the most important native aquaculture species in Brazil. Data for generating the draft assembly were produced from shotgun libraries with two different insert sizes and mate-paired libraries with four different sizes sequenced (2x150bps) with Illumina HiSeq2000 technology. A total of 124.8Gbp quality-filtered nucleotides were sequenced which amount to 85x mean genome coverage, considering previously published information (C-value = 1,5pg = 1.467Gbp). Sequence assembly was performed with SOAPdenovo and generated 8.924 scaffolds spanning 1.54 Gbp (N50: 2,041,733bp (162 scaffolds), N90: 200,945bp (1009 scaffolds), ~500Mbp of unmapped nucleotides). Gene model prediction is underway with MAKER2 using as extrinsic evidence protein and EST data from phylogenetically related taxa. This represents the first report of a draft genome sequence for this species and will be a valuable source of information for marker detection/selection, genetic improvement, conservation and basic biology studies in this species. PAG 2015. Pôster P0231. 2015-04-10T11:11:11Z 2015-04-10T11:11:11Z 2015-04-10 2015 2020-01-21T11:11:11Z Anais e Proceedings de eventos In: PLANT & ANIMAL GENOME CONFERENCE, 23., 2015, San Diego, CA. [Abstracts...]. San Diego: [s.n.], 2015. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1013201 en eng openAccess não paginado. |
institution |
EMBRAPA |
collection |
DSpace |
country |
Brasil |
countrycode |
BR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-alice |
tag |
biblioteca |
region |
America del Sur |
libraryname |
Sistema de bibliotecas de EMBRAPA |
language |
English eng |
topic |
Sequência genômica. Tambaqui Colossoma Macropomum. Genome assembly Sequence analysis. Sequência genômica. Tambaqui Colossoma Macropomum. Genome assembly Sequence analysis. |
spellingShingle |
Sequência genômica. Tambaqui Colossoma Macropomum. Genome assembly Sequence analysis. Sequência genômica. Tambaqui Colossoma Macropomum. Genome assembly Sequence analysis. LOBO, F. P. CINTRA, L. C. VARELA, E. S. ALVES, A. L. VILLELA, L. C. V. SILVA, N. M. A. da PAIVA, S. R. CAETANO, A. R. de novo genome assembly of the South American freshwater fish Tambaqui (Colossoma macropomum). |
description |
The Tambaqui (Colossoma macropomum) is a freshwater fish species naturally found in the Amazon river basin which has historically been widely exploited by community and commercial fishing. Recent efforts to domesticate, breed and raise the species in aquaculture systems has led to significant increases in production (>10-fold) over the last ten years. Current production is above 120,000 metric tons per year with a strong growth trend, making it the most important native aquaculture species in Brazil. Data for generating the draft assembly were produced from shotgun libraries with two different insert sizes and mate-paired libraries with four different sizes sequenced (2x150bps) with Illumina HiSeq2000 technology. A total of 124.8Gbp quality-filtered nucleotides were sequenced which amount to 85x mean genome coverage, considering previously published information (C-value = 1,5pg = 1.467Gbp). Sequence assembly was performed with SOAPdenovo and generated 8.924 scaffolds spanning 1.54 Gbp (N50: 2,041,733bp (162 scaffolds), N90: 200,945bp (1009 scaffolds), ~500Mbp of unmapped nucleotides). Gene model prediction is underway with MAKER2 using as extrinsic evidence protein and EST data from phylogenetically related taxa. This represents the first report of a draft genome sequence for this species and will be a valuable source of information for marker detection/selection, genetic improvement, conservation and basic biology studies in this species. |
author2 |
FRANCISCO PEREIRA LOBO, CNPTIA; LEANDRO CARRIJO CINTRA, CNPTIA; EDUARDO SOUSA VARELA, CNPASA; ANDERSON LUIS ALVES, CNPASA; LUCIANA CRISTINE VASQUES VILLELA, UnB; NAIARA MILAGRES AUGUSTO DA SILVA, CENARGEN; SAMUEL REZENDE PAIVA, SRI; ALEXANDRE RODRIGUES CAETANO, CENARGEN. |
author_facet |
FRANCISCO PEREIRA LOBO, CNPTIA; LEANDRO CARRIJO CINTRA, CNPTIA; EDUARDO SOUSA VARELA, CNPASA; ANDERSON LUIS ALVES, CNPASA; LUCIANA CRISTINE VASQUES VILLELA, UnB; NAIARA MILAGRES AUGUSTO DA SILVA, CENARGEN; SAMUEL REZENDE PAIVA, SRI; ALEXANDRE RODRIGUES CAETANO, CENARGEN. LOBO, F. P. CINTRA, L. C. VARELA, E. S. ALVES, A. L. VILLELA, L. C. V. SILVA, N. M. A. da PAIVA, S. R. CAETANO, A. R. |
format |
Anais e Proceedings de eventos |
topic_facet |
Sequência genômica. Tambaqui Colossoma Macropomum. Genome assembly Sequence analysis. |
author |
LOBO, F. P. CINTRA, L. C. VARELA, E. S. ALVES, A. L. VILLELA, L. C. V. SILVA, N. M. A. da PAIVA, S. R. CAETANO, A. R. |
author_sort |
LOBO, F. P. |
title |
de novo genome assembly of the South American freshwater fish Tambaqui (Colossoma macropomum). |
title_short |
de novo genome assembly of the South American freshwater fish Tambaqui (Colossoma macropomum). |
title_full |
de novo genome assembly of the South American freshwater fish Tambaqui (Colossoma macropomum). |
title_fullStr |
de novo genome assembly of the South American freshwater fish Tambaqui (Colossoma macropomum). |
title_full_unstemmed |
de novo genome assembly of the South American freshwater fish Tambaqui (Colossoma macropomum). |
title_sort |
de novo genome assembly of the south american freshwater fish tambaqui (colossoma macropomum). |
publishDate |
2015-04-10 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1013201 |
work_keys_str_mv |
AT lobofp denovogenomeassemblyofthesouthamericanfreshwaterfishtambaquicolossomamacropomum AT cintralc denovogenomeassemblyofthesouthamericanfreshwaterfishtambaquicolossomamacropomum AT varelaes denovogenomeassemblyofthesouthamericanfreshwaterfishtambaquicolossomamacropomum AT alvesal denovogenomeassemblyofthesouthamericanfreshwaterfishtambaquicolossomamacropomum AT villelalcv denovogenomeassemblyofthesouthamericanfreshwaterfishtambaquicolossomamacropomum AT silvanmada denovogenomeassemblyofthesouthamericanfreshwaterfishtambaquicolossomamacropomum AT paivasr denovogenomeassemblyofthesouthamericanfreshwaterfishtambaquicolossomamacropomum AT caetanoar denovogenomeassemblyofthesouthamericanfreshwaterfishtambaquicolossomamacropomum |
_version_ |
1756020854588702720 |