Leaf and root transcriptome sequencing of tomato accessions
RNAseq was performed on leaf and root tissue from two accessions of tomato to identify NLRs and their relative expression level. Leaves and roots were sampled at one month post germination. Leaf tissue was collected from all fully expanded leaves, whereas roots were sampled and washed in distilled water. Accessions were sequenced using 150 bp paired end reads. RNAseq read quality was assessed and trimmed using Trimmomatic (version 0.36) with the parameters ILLUMINACLIP:2:30:10, LEADING:3, TRAILING:3, SLIDINGWINDOW:4:15, and MINLEN:100. Transcriptome assembly was performed using Trinity (version 2013-11-10) with default parameters.
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Format: | Dataset biblioteca |
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2023
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Subjects: | Genetics, Solanum lycopersicum, eEukaryotes, raw sequence reads, assembly, |
Online Access: | https://figshare.com/articles/dataset/Leaf_and_root_transcriptome_sequencing_of_tomato_accessions/25156064 |
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dat-usda-us-article251560642023-01-24T00:00:00Z Leaf and root transcriptome sequencing of tomato accessions United States Department of Agriculture (18794677) Genetics Solanum lycopersicum eEukaryotes raw sequence reads assembly RNAseq was performed on leaf and root tissue from two accessions of tomato to identify NLRs and their relative expression level. Leaves and roots were sampled at one month post germination. Leaf tissue was collected from all fully expanded leaves, whereas roots were sampled and washed in distilled water. Accessions were sequenced using 150 bp paired end reads. RNAseq read quality was assessed and trimmed using Trimmomatic (version 0.36) with the parameters ILLUMINACLIP:2:30:10, LEADING:3, TRAILING:3, SLIDINGWINDOW:4:15, and MINLEN:100. Transcriptome assembly was performed using Trinity (version 2013-11-10) with default parameters. 2023-01-24T00:00:00Z Dataset Dataset 10113/AF25156064 https://figshare.com/articles/dataset/Leaf_and_root_transcriptome_sequencing_of_tomato_accessions/25156064 Copyright Undetermined |
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National Agricultural Library of USDA |
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Genetics Solanum lycopersicum eEukaryotes raw sequence reads assembly |
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Genetics Solanum lycopersicum eEukaryotes raw sequence reads assembly United States Department of Agriculture (18794677) Leaf and root transcriptome sequencing of tomato accessions |
description |
RNAseq was performed on leaf and root tissue from two accessions of tomato to identify NLRs and their relative expression level. Leaves and roots were sampled at one month post germination. Leaf tissue was collected from all fully expanded leaves, whereas roots were sampled and washed in distilled water. Accessions were sequenced using 150 bp paired end reads. RNAseq read quality was assessed and trimmed using Trimmomatic (version 0.36) with the parameters ILLUMINACLIP:2:30:10, LEADING:3, TRAILING:3, SLIDINGWINDOW:4:15, and MINLEN:100. Transcriptome assembly was performed using Trinity (version 2013-11-10) with default parameters. |
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United States Department of Agriculture (18794677) |
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United States Department of Agriculture (18794677) |
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United States Department of Agriculture (18794677) |
title |
Leaf and root transcriptome sequencing of tomato accessions |
title_short |
Leaf and root transcriptome sequencing of tomato accessions |
title_full |
Leaf and root transcriptome sequencing of tomato accessions |
title_fullStr |
Leaf and root transcriptome sequencing of tomato accessions |
title_full_unstemmed |
Leaf and root transcriptome sequencing of tomato accessions |
title_sort |
leaf and root transcriptome sequencing of tomato accessions |
publishDate |
2023 |
url |
https://figshare.com/articles/dataset/Leaf_and_root_transcriptome_sequencing_of_tomato_accessions/25156064 |
work_keys_str_mv |
AT unitedstatesdepartmentofagriculture18794677 leafandroottranscriptomesequencingoftomatoaccessions |
_version_ |
1807157368632377344 |