Using next generation sequencing to compare gene expression of wild type and Δcsp1 mutant of bacterial plant pathogen Xylella fastidiosa strain Stag's Leap

To investigate the role(s) of a cold shock protein homolog (Csp1) in plant pathogenic bacteria Xylella fastidiosa, we compared transcriptome profiles between wild type and a csp1 deletion mutant (Δcsp1) using long read Nanopore RNA sequencing. Overall design: Examined mRNA profiles of 7 day old wild type and Δcsp1 X. fastidiosa cells grown on PD3 agar plates under standard conditions (28°C) and at low temperature conditions (15°C)

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Bibliographic Details
Main Author: USDA-ARS (17854919)
Format: Dataset biblioteca
Published: 2021
Subjects:Genetics, Xylella fastidiosa subsp. fastidiosa, eBacteria,
Online Access:https://figshare.com/articles/dataset/Using_next_generation_sequencing_to_compare_gene_expression_of_wild_type_and_csp1_mutant_of_bacterial_plant_pathogen_Xylella_fastidiosa_strain_Stag_s_Leap/25088987
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Description
Summary:To investigate the role(s) of a cold shock protein homolog (Csp1) in plant pathogenic bacteria Xylella fastidiosa, we compared transcriptome profiles between wild type and a csp1 deletion mutant (Δcsp1) using long read Nanopore RNA sequencing. Overall design: Examined mRNA profiles of 7 day old wild type and Δcsp1 X. fastidiosa cells grown on PD3 agar plates under standard conditions (28°C) and at low temperature conditions (15°C)