Unravelling the trans-kingdom function of Sstrf2, tRNA-derived small RNA of Sclerotinia sclerotiorum

In Sclerotinia sclerotiorum, white mold fungus, tRNA-derived small RNAs (tRFs) potentially belong to a new class of virulence effector because we previously reported two very abundant of larger size at 28-30 nt. In this study, we show that the tRFs have no fungal targets predicted based on transcriptome, degradome and small RNA-Seq of S. sclerotiorum. The tRFs function in a trans-kingdom manner was also evidenced by immunoprecipitation of argonaute protein 1 (Ago1) profiled by small RNA-Seq, as well as A. thaliana ago1 knockout mutant is more tolerant to DK3 inoculation.

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Bibliographic Details
Main Author: USDA ARS (18794668)
Format: Dataset biblioteca
Published: 2021
Subjects:Genetics, raw sequence reads,
Online Access:https://figshare.com/articles/dataset/Unravelling_the_trans-kingdom_function_of_Sstrf2_tRNA-derived_small_RNA_of_Sclerotinia_sclerotiorum/25088831
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spelling dat-usda-us-article250888312021-08-04T00:00:00Z Unravelling the trans-kingdom function of Sstrf2, tRNA-derived small RNA of Sclerotinia sclerotiorum USDA ARS (18794668) Genetics raw sequence reads In Sclerotinia sclerotiorum, white mold fungus, tRNA-derived small RNAs (tRFs) potentially belong to a new class of virulence effector because we previously reported two very abundant of larger size at 28-30 nt. In this study, we show that the tRFs have no fungal targets predicted based on transcriptome, degradome and small RNA-Seq of S. sclerotiorum. The tRFs function in a trans-kingdom manner was also evidenced by immunoprecipitation of argonaute protein 1 (Ago1) profiled by small RNA-Seq, as well as A. thaliana ago1 knockout mutant is more tolerant to DK3 inoculation. 2021-08-04T00:00:00Z Dataset Dataset 10113/AF25088831 https://figshare.com/articles/dataset/Unravelling_the_trans-kingdom_function_of_Sstrf2_tRNA-derived_small_RNA_of_Sclerotinia_sclerotiorum/25088831 Copyright Undetermined
institution USDA US
collection Figshare
country Estados Unidos
countrycode US
component Datos de investigación
access En linea
databasecode dat-usda-us
tag biblioteca
region America del Norte
libraryname National Agricultural Library of USDA
topic Genetics
raw sequence reads
spellingShingle Genetics
raw sequence reads
USDA ARS (18794668)
Unravelling the trans-kingdom function of Sstrf2, tRNA-derived small RNA of Sclerotinia sclerotiorum
description In Sclerotinia sclerotiorum, white mold fungus, tRNA-derived small RNAs (tRFs) potentially belong to a new class of virulence effector because we previously reported two very abundant of larger size at 28-30 nt. In this study, we show that the tRFs have no fungal targets predicted based on transcriptome, degradome and small RNA-Seq of S. sclerotiorum. The tRFs function in a trans-kingdom manner was also evidenced by immunoprecipitation of argonaute protein 1 (Ago1) profiled by small RNA-Seq, as well as A. thaliana ago1 knockout mutant is more tolerant to DK3 inoculation.
format Dataset
author USDA ARS (18794668)
author_facet USDA ARS (18794668)
author_sort USDA ARS (18794668)
title Unravelling the trans-kingdom function of Sstrf2, tRNA-derived small RNA of Sclerotinia sclerotiorum
title_short Unravelling the trans-kingdom function of Sstrf2, tRNA-derived small RNA of Sclerotinia sclerotiorum
title_full Unravelling the trans-kingdom function of Sstrf2, tRNA-derived small RNA of Sclerotinia sclerotiorum
title_fullStr Unravelling the trans-kingdom function of Sstrf2, tRNA-derived small RNA of Sclerotinia sclerotiorum
title_full_unstemmed Unravelling the trans-kingdom function of Sstrf2, tRNA-derived small RNA of Sclerotinia sclerotiorum
title_sort unravelling the trans-kingdom function of sstrf2, trna-derived small rna of sclerotinia sclerotiorum
publishDate 2021
url https://figshare.com/articles/dataset/Unravelling_the_trans-kingdom_function_of_Sstrf2_tRNA-derived_small_RNA_of_Sclerotinia_sclerotiorum/25088831
work_keys_str_mv AT usdaars18794668 unravellingthetranskingdomfunctionofsstrf2trnaderivedsmallrnaofsclerotiniasclerotiorum
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