Unravelling the trans-kingdom function of Sstrf2, tRNA-derived small RNA of Sclerotinia sclerotiorum
In Sclerotinia sclerotiorum, white mold fungus, tRNA-derived small RNAs (tRFs) potentially belong to a new class of virulence effector because we previously reported two very abundant of larger size at 28-30 nt. In this study, we show that the tRFs have no fungal targets predicted based on transcriptome, degradome and small RNA-Seq of S. sclerotiorum. The tRFs function in a trans-kingdom manner was also evidenced by immunoprecipitation of argonaute protein 1 (Ago1) profiled by small RNA-Seq, as well as A. thaliana ago1 knockout mutant is more tolerant to DK3 inoculation.
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Format: | Dataset biblioteca |
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2021
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Subjects: | Genetics, raw sequence reads, |
Online Access: | https://figshare.com/articles/dataset/Unravelling_the_trans-kingdom_function_of_Sstrf2_tRNA-derived_small_RNA_of_Sclerotinia_sclerotiorum/25088831 |
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dat-usda-us-article250888312021-08-04T00:00:00Z Unravelling the trans-kingdom function of Sstrf2, tRNA-derived small RNA of Sclerotinia sclerotiorum USDA ARS (18794668) Genetics raw sequence reads In Sclerotinia sclerotiorum, white mold fungus, tRNA-derived small RNAs (tRFs) potentially belong to a new class of virulence effector because we previously reported two very abundant of larger size at 28-30 nt. In this study, we show that the tRFs have no fungal targets predicted based on transcriptome, degradome and small RNA-Seq of S. sclerotiorum. The tRFs function in a trans-kingdom manner was also evidenced by immunoprecipitation of argonaute protein 1 (Ago1) profiled by small RNA-Seq, as well as A. thaliana ago1 knockout mutant is more tolerant to DK3 inoculation. 2021-08-04T00:00:00Z Dataset Dataset 10113/AF25088831 https://figshare.com/articles/dataset/Unravelling_the_trans-kingdom_function_of_Sstrf2_tRNA-derived_small_RNA_of_Sclerotinia_sclerotiorum/25088831 Copyright Undetermined |
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Genetics raw sequence reads USDA ARS (18794668) Unravelling the trans-kingdom function of Sstrf2, tRNA-derived small RNA of Sclerotinia sclerotiorum |
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In Sclerotinia sclerotiorum, white mold fungus, tRNA-derived small RNAs (tRFs) potentially belong to a new class of virulence effector because we previously reported two very abundant of larger size at 28-30 nt. In this study, we show that the tRFs have no fungal targets predicted based on transcriptome, degradome and small RNA-Seq of S. sclerotiorum. The tRFs function in a trans-kingdom manner was also evidenced by immunoprecipitation of argonaute protein 1 (Ago1) profiled by small RNA-Seq, as well as A. thaliana ago1 knockout mutant is more tolerant to DK3 inoculation. |
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USDA ARS (18794668) |
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USDA ARS (18794668) |
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USDA ARS (18794668) |
title |
Unravelling the trans-kingdom function of Sstrf2, tRNA-derived small RNA of Sclerotinia sclerotiorum |
title_short |
Unravelling the trans-kingdom function of Sstrf2, tRNA-derived small RNA of Sclerotinia sclerotiorum |
title_full |
Unravelling the trans-kingdom function of Sstrf2, tRNA-derived small RNA of Sclerotinia sclerotiorum |
title_fullStr |
Unravelling the trans-kingdom function of Sstrf2, tRNA-derived small RNA of Sclerotinia sclerotiorum |
title_full_unstemmed |
Unravelling the trans-kingdom function of Sstrf2, tRNA-derived small RNA of Sclerotinia sclerotiorum |
title_sort |
unravelling the trans-kingdom function of sstrf2, trna-derived small rna of sclerotinia sclerotiorum |
publishDate |
2021 |
url |
https://figshare.com/articles/dataset/Unravelling_the_trans-kingdom_function_of_Sstrf2_tRNA-derived_small_RNA_of_Sclerotinia_sclerotiorum/25088831 |
work_keys_str_mv |
AT usdaars18794668 unravellingthetranskingdomfunctionofsstrf2trnaderivedsmallrnaofsclerotiniasclerotiorum |
_version_ |
1813294112732872704 |