Unravelling the trans-kingdom function of Sstrf2, tRNA-derived small RNA of Sclerotinia sclerotiorum

In Sclerotinia sclerotiorum, white mold fungus, tRNA-derived small RNAs (tRFs) potentially belong to a new class of virulence effector because we previously reported two very abundant of larger size at 28-30 nt. In this study, we show that the tRFs have no fungal targets predicted based on transcriptome, degradome and small RNA-Seq of S. sclerotiorum. The tRFs function in a trans-kingdom manner was also evidenced by immunoprecipitation of argonaute protein 1 (Ago1) profiled by small RNA-Seq, as well as A. thaliana ago1 knockout mutant is more tolerant to DK3 inoculation.

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Bibliographic Details
Main Author: USDA ARS (18794668)
Format: Dataset biblioteca
Published: 2021
Subjects:Genetics, raw sequence reads,
Online Access:https://figshare.com/articles/dataset/Unravelling_the_trans-kingdom_function_of_Sstrf2_tRNA-derived_small_RNA_of_Sclerotinia_sclerotiorum/25088831
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Description
Summary:In Sclerotinia sclerotiorum, white mold fungus, tRNA-derived small RNAs (tRFs) potentially belong to a new class of virulence effector because we previously reported two very abundant of larger size at 28-30 nt. In this study, we show that the tRFs have no fungal targets predicted based on transcriptome, degradome and small RNA-Seq of S. sclerotiorum. The tRFs function in a trans-kingdom manner was also evidenced by immunoprecipitation of argonaute protein 1 (Ago1) profiled by small RNA-Seq, as well as A. thaliana ago1 knockout mutant is more tolerant to DK3 inoculation.