TnSeq mapping and BarSeq quantification of Ralstonia mutant fitness

TnSeq mapping and BarSeq quantification of Ralstonia mutant fitness

We created transposon insertion libraries in 3 plant pathogenic Ralstonia isolates using pKMW3, a randomly barcoded Mariner transposon vector. Mutant libraries were created in R. pseudosolanacearum GMI1000, R. solanacearum IBSBF1503, and R. syzygii PSI07. (Note: The genomes for several strains are still classified as R. solanacearum, but the names here reflect the current taxonomy). See PMID: 25968644 for plasmid reference, and TnSeq / BarSeq protocols.

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Bibliographic Details
Main Authors: USDA-ARS (17854919), QB3 Berkeley Genomics (18796879)
Format: Dataset biblioteca
Published: 2020
Subjects:Genetics, raw sequence reads,
Online Access:https://figshare.com/articles/dataset/TnSeq_mapping_and_BarSeq_quantification_of_Ralstonia_mutant_fitness/25086542
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id dat-usda-us-article25086542
record_format figshare
spelling dat-usda-us-article250865422020-04-28T00:00:00Z TnSeq mapping and BarSeq quantification of Ralstonia mutant fitness USDA-ARS (17854919) QB3 Berkeley Genomics (18796879) Genetics raw sequence reads We created transposon insertion libraries in 3 plant pathogenic Ralstonia isolates using pKMW3, a randomly barcoded Mariner transposon vector. Mutant libraries were created in R. pseudosolanacearum GMI1000, R. solanacearum IBSBF1503, and R. syzygii PSI07. (Note: The genomes for several strains are still classified as R. solanacearum, but the names here reflect the current taxonomy). See PMID: 25968644 for plasmid reference, and TnSeq / BarSeq protocols. 2020-04-28T00:00:00Z Dataset Dataset 10113/AF25086542 https://figshare.com/articles/dataset/TnSeq_mapping_and_BarSeq_quantification_of_Ralstonia_mutant_fitness/25086542 Copyright Undetermined
institution USDA US
collection Figshare
country Estados Unidos
countrycode US
component Datos de investigación
access En linea
databasecode dat-usda-us
tag biblioteca
region America del Norte
libraryname National Agricultural Library of USDA
topic Genetics
raw sequence reads
spellingShingle Genetics
raw sequence reads
USDA-ARS (17854919)
QB3 Berkeley Genomics (18796879)
TnSeq mapping and BarSeq quantification of Ralstonia mutant fitness
description We created transposon insertion libraries in 3 plant pathogenic Ralstonia isolates using pKMW3, a randomly barcoded Mariner transposon vector. Mutant libraries were created in R. pseudosolanacearum GMI1000, R. solanacearum IBSBF1503, and R. syzygii PSI07. (Note: The genomes for several strains are still classified as R. solanacearum, but the names here reflect the current taxonomy). See PMID: 25968644 for plasmid reference, and TnSeq / BarSeq protocols.
format Dataset
author USDA-ARS (17854919)
QB3 Berkeley Genomics (18796879)
author_facet USDA-ARS (17854919)
QB3 Berkeley Genomics (18796879)
author_sort USDA-ARS (17854919)
title TnSeq mapping and BarSeq quantification of Ralstonia mutant fitness
title_short TnSeq mapping and BarSeq quantification of Ralstonia mutant fitness
title_full TnSeq mapping and BarSeq quantification of Ralstonia mutant fitness
title_fullStr TnSeq mapping and BarSeq quantification of Ralstonia mutant fitness
title_full_unstemmed TnSeq mapping and BarSeq quantification of Ralstonia mutant fitness
title_sort tnseq mapping and barseq quantification of ralstonia mutant fitness
publishDate 2020
url https://figshare.com/articles/dataset/TnSeq_mapping_and_BarSeq_quantification_of_Ralstonia_mutant_fitness/25086542
work_keys_str_mv AT usdaars17854919 tnseqmappingandbarseqquantificationofralstoniamutantfitness
AT qb3berkeleygenomics18796879 tnseqmappingandbarseqquantificationofralstoniamutantfitness
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