Soybean response to brown stem rot fungus infection
Brown stem rot (BSR), caused by the fungus Phialophora gregata, reduces yield by 38%. Three dominant BSR resistance loci have been identified: Rbs1, Rbs2, and Rbs3. All three genes have been mapped to large, overlapping regions on soybean chromosome 16 though the gene networks regulating resistance remain unknown. Further, identifying resistant germplasm by genotyping or phenotyping remains difficult due to complexities of soybean/P. gregata interactions. To aid in identifying and characterizing soybean resistance genes, novel approaches bridging the gap between breeding and genomics are needed. To identify genes and gene networks important in the resistant response, I conducted RNA-Seq of leaf, stem, and root tissues collected 12, 24, and 36 hours after P. gregata or mock-infection of a Rbs3 resistant soybean genotype. I identified genes differentially expressed in response to infection (treatment) at each time point.
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| Format: | Dataset biblioteca |
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2019
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| Subjects: | Genetics, raw sequence reads, |
| Online Access: | https://figshare.com/articles/dataset/Soybean_response_to_brown_stem_rot_fungus_infection/25085531 |
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dat-usda-us-article250855312019-06-12T00:00:00Z Soybean response to brown stem rot fungus infection USDA ARS (18794668) Genetics raw sequence reads Brown stem rot (BSR), caused by the fungus Phialophora gregata, reduces yield by 38%. Three dominant BSR resistance loci have been identified: Rbs1, Rbs2, and Rbs3. All three genes have been mapped to large, overlapping regions on soybean chromosome 16 though the gene networks regulating resistance remain unknown. Further, identifying resistant germplasm by genotyping or phenotyping remains difficult due to complexities of soybean/P. gregata interactions. To aid in identifying and characterizing soybean resistance genes, novel approaches bridging the gap between breeding and genomics are needed. To identify genes and gene networks important in the resistant response, I conducted RNA-Seq of leaf, stem, and root tissues collected 12, 24, and 36 hours after P. gregata or mock-infection of a Rbs3 resistant soybean genotype. I identified genes differentially expressed in response to infection (treatment) at each time point. 2019-06-12T00:00:00Z Dataset Dataset 10113/AF25085531 https://figshare.com/articles/dataset/Soybean_response_to_brown_stem_rot_fungus_infection/25085531 Copyright Undetermined |
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America del Norte |
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National Agricultural Library of USDA |
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Genetics raw sequence reads |
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Genetics raw sequence reads USDA ARS (18794668) Soybean response to brown stem rot fungus infection |
| description |
Brown stem rot (BSR), caused by the fungus Phialophora gregata, reduces yield by 38%. Three dominant BSR resistance loci have been identified: Rbs1, Rbs2, and Rbs3. All three genes have been mapped to large, overlapping regions on soybean chromosome 16 though the gene networks regulating resistance remain unknown. Further, identifying resistant germplasm by genotyping or phenotyping remains difficult due to complexities of soybean/P. gregata interactions. To aid in identifying and characterizing soybean resistance genes, novel approaches bridging the gap between breeding and genomics are needed. To identify genes and gene networks important in the resistant response, I conducted RNA-Seq of leaf, stem, and root tissues collected 12, 24, and 36 hours after P. gregata or mock-infection of a Rbs3 resistant soybean genotype. I identified genes differentially expressed in response to infection (treatment) at each time point. |
| format |
Dataset |
| author |
USDA ARS (18794668) |
| author_facet |
USDA ARS (18794668) |
| author_sort |
USDA ARS (18794668) |
| title |
Soybean response to brown stem rot fungus infection |
| title_short |
Soybean response to brown stem rot fungus infection |
| title_full |
Soybean response to brown stem rot fungus infection |
| title_fullStr |
Soybean response to brown stem rot fungus infection |
| title_full_unstemmed |
Soybean response to brown stem rot fungus infection |
| title_sort |
soybean response to brown stem rot fungus infection |
| publishDate |
2019 |
| url |
https://figshare.com/articles/dataset/Soybean_response_to_brown_stem_rot_fungus_infection/25085531 |
| work_keys_str_mv |
AT usdaars18794668 soybeanresponsetobrownstemrotfungusinfection |
| _version_ |
1813294335000576000 |