Profiling of gut gene expression in Cry1Ab-resistant and susceptible strains of Ostrinia nubilalis reveals possible adaptations of resistant larvae to transgenic Cry1Ab corn
We revealed a total of 398 gut genes differentially expressed (i.e., either up- or down-regulated genes with expression ratio ≥2.0) in S-strain larvae, while only 264 gut genes differentially expressed in R-strain larvae after fed transgenic corn leaves. Although the percentages of down-regulated genes among the total number of differentially expressed genes (199 genes or 50% in S-strain; 119 genes or 45% in R-strain) were similar between the R and S-strains, the expression ratios of down-regulated genes were much higher in S-strain than in R-strain. These results imply that R-strain larvae had an increased ability to deal with transgenic corn as compared with S-strain larvae. We also revealed that 17 and 9 significantly up- or down-regulated gut genes from S- and R-strain larvae, respectively, including serine proteases (trypsins and chymotrypsins) and aminopeptidases. Overall design: Trangenic corn induced gene expression was measured in O. nubilalis resistant and susceptible strains' gut at 6-hrs after transgenic corn feeding. Three independent experiments were performed using three insect gut samples ,and each sample was from five insects' gut pooled.
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Format: | Dataset biblioteca |
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2016
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Subjects: | Genetics, Ostrinia nubilalis, eEukaryotes, |
Online Access: | https://figshare.com/articles/dataset/Profiling_of_gut_gene_expression_in_Cry1Ab-resistant_and_susceptible_strains_of_Ostrinia_nubilalis_reveals_possible_adaptations_of_resistant_larvae_to_transgenic_Cry1Ab_corn/25081532 |
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dat-usda-us-article250815322016-09-27T00:00:00Z Profiling of gut gene expression in Cry1Ab-resistant and susceptible strains of Ostrinia nubilalis reveals possible adaptations of resistant larvae to transgenic Cry1Ab corn USDA-ARS (17854919) Genetics Ostrinia nubilalis eEukaryotes We revealed a total of 398 gut genes differentially expressed (i.e., either up- or down-regulated genes with expression ratio ≥2.0) in S-strain larvae, while only 264 gut genes differentially expressed in R-strain larvae after fed transgenic corn leaves. Although the percentages of down-regulated genes among the total number of differentially expressed genes (199 genes or 50% in S-strain; 119 genes or 45% in R-strain) were similar between the R and S-strains, the expression ratios of down-regulated genes were much higher in S-strain than in R-strain. These results imply that R-strain larvae had an increased ability to deal with transgenic corn as compared with S-strain larvae. We also revealed that 17 and 9 significantly up- or down-regulated gut genes from S- and R-strain larvae, respectively, including serine proteases (trypsins and chymotrypsins) and aminopeptidases. Overall design: Trangenic corn induced gene expression was measured in O. nubilalis resistant and susceptible strains' gut at 6-hrs after transgenic corn feeding. Three independent experiments were performed using three insect gut samples ,and each sample was from five insects' gut pooled. 2016-09-27T00:00:00Z Dataset Dataset 10113/AF25081532 https://figshare.com/articles/dataset/Profiling_of_gut_gene_expression_in_Cry1Ab-resistant_and_susceptible_strains_of_Ostrinia_nubilalis_reveals_possible_adaptations_of_resistant_larvae_to_transgenic_Cry1Ab_corn/25081532 Copyright Undetermined |
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Genetics Ostrinia nubilalis eEukaryotes USDA-ARS (17854919) Profiling of gut gene expression in Cry1Ab-resistant and susceptible strains of Ostrinia nubilalis reveals possible adaptations of resistant larvae to transgenic Cry1Ab corn |
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We revealed a total of 398 gut genes differentially expressed (i.e., either up- or down-regulated genes with expression ratio ≥2.0) in S-strain larvae, while only 264 gut genes differentially expressed in R-strain larvae after fed transgenic corn leaves. Although the percentages of down-regulated genes among the total number of differentially expressed genes (199 genes or 50% in S-strain; 119 genes or 45% in R-strain) were similar between the R and S-strains, the expression ratios of down-regulated genes were much higher in S-strain than in R-strain. These results imply that R-strain larvae had an increased ability to deal with transgenic corn as compared with S-strain larvae. We also revealed that 17 and 9 significantly up- or down-regulated gut genes from S- and R-strain larvae, respectively, including serine proteases (trypsins and chymotrypsins) and aminopeptidases. Overall design: Trangenic corn induced gene expression was measured in O. nubilalis resistant and susceptible strains' gut at 6-hrs after transgenic corn feeding. Three independent experiments were performed using three insect gut samples ,and each sample was from five insects' gut pooled. |
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title |
Profiling of gut gene expression in Cry1Ab-resistant and susceptible strains of Ostrinia nubilalis reveals possible adaptations of resistant larvae to transgenic Cry1Ab corn |
title_short |
Profiling of gut gene expression in Cry1Ab-resistant and susceptible strains of Ostrinia nubilalis reveals possible adaptations of resistant larvae to transgenic Cry1Ab corn |
title_full |
Profiling of gut gene expression in Cry1Ab-resistant and susceptible strains of Ostrinia nubilalis reveals possible adaptations of resistant larvae to transgenic Cry1Ab corn |
title_fullStr |
Profiling of gut gene expression in Cry1Ab-resistant and susceptible strains of Ostrinia nubilalis reveals possible adaptations of resistant larvae to transgenic Cry1Ab corn |
title_full_unstemmed |
Profiling of gut gene expression in Cry1Ab-resistant and susceptible strains of Ostrinia nubilalis reveals possible adaptations of resistant larvae to transgenic Cry1Ab corn |
title_sort |
profiling of gut gene expression in cry1ab-resistant and susceptible strains of ostrinia nubilalis reveals possible adaptations of resistant larvae to transgenic cry1ab corn |
publishDate |
2016 |
url |
https://figshare.com/articles/dataset/Profiling_of_gut_gene_expression_in_Cry1Ab-resistant_and_susceptible_strains_of_Ostrinia_nubilalis_reveals_possible_adaptations_of_resistant_larvae_to_transgenic_Cry1Ab_corn/25081532 |
work_keys_str_mv |
AT usdaars17854919 profilingofgutgeneexpressionincry1abresistantandsusceptiblestrainsofostrinianubilalisrevealspossibleadaptationsofresistantlarvaetotransgeniccry1abcorn |
_version_ |
1808945301689466880 |