Data from: Attraction, mobility, and preference by Lasioderma serricorne (F.) (Coleoptera: Ptinidae) to microbially-mediated volatile emissions by two species of fungi in stored grain
<p>Our goals were to 1) isolate, and culture two fungal morphotypes, 2) characterize the volatile emissions from grain inoculated by each fungal morphotype (<em>Aspergillus flavus</em> or <em>Fusarium</em> spp.) compared to uninoculated and sanitized grain, and 3) understand how MVOCs from each morphotype affects mobility, attraction, and preference by <em>L. serricorne</em>. Headspace collection revealed that the <em>Fusarium</em>- and <em>A. flavus</em>-inoculated grain produced significantly different volatiles compared to sanitized grain or the positive control. Changes in MVOC emissions affected close-range foraging during an Ethovision assay, with a greater frequency of entering and spending time in a small zone with kernels inoculated with <em>A. flavus</em> compared to other treatments. In the release-recapture assay, MVOCs were found to be attractive to <em>L. serricorne</em> at a longer distances in commercial pitfall traps. While there was no preference shown among semiochemical stimuli in a still-air, four-way olfactometer, it is possible that methodological limitations prevented robust interpretation from this assay. Overall, our study suggests that MVOCs are important for close- and long-range orientation of <em>L.serricorne</em> during foraging, and that MVOCs may have the potential for inclusion in behaviorally-based tactics for this species.</p> <div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: 4-way olfactometer assay.</p> <p>File Name: Sierra_2021_olfactometer_Ag_Data_commons.csv</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/en-us/microsoft-365/excel" target="_blank">https://www.microsoft.com/en-us/microsoft-365/excel</a> </p></li><br><li><p>Resource Title: Release-recapture Assay .</p> <p>File Name: sierra_release_recapture_exp_2021_fungal_volatiles_agdata_commons.csv</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/en-us/microsoft-365/excel" target="_blank">https://www.microsoft.com/en-us/microsoft-365/excel</a> </p></li><br><li><p>Resource Title: Ethovision Movement Assay .</p> <p>File Name: ethovision_sierra_2021_microbial_volatiles_agdatacommons.csv</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/en-us/microsoft-365/excel" target="_blank">https://www.microsoft.com/en-us/microsoft-365/excel</a> </p></li><br><li><p>Resource Title: Headspace volatile collection assay.</p> <p>File Name: headspace_compounds_sierra_2021_fungal_volatiles_final_agdatacommons.csv</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/en-us/microsoft-365/excel" target="_blank">https://www.microsoft.com/en-us/microsoft-365/excel</a> </p></li><br><li><p>Resource Title: Sequencing Data.</p> <p>File Name: sequencing_data.zip</p></li><br><li><p>Resource Title: File list.</p> <p>File Name: File_list_L_serricone_attraction_data.txt</p></li></ul>
| Main Authors: | , , , , , |
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| Format: | Dataset biblioteca |
| Published: |
2023
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| Subjects: | Agricultural, veterinary and food sciences, Crop and pasture production, Crop and pasture protection (incl. pests, diseases and weeds), Environmental sciences, behavioral ecology, stored products, MVOCs, Coleoptera, volatile responses, microbes, mobility, Insects, entomology, olfaction, trapping, IPM, NP304, data.gov, ARS, |
| Online Access: | https://figshare.com/articles/dataset/Data_from_Attraction_mobility_and_preference_by_Lasioderma_serricorne_F_Coleoptera_Ptinidae_to_microbially-mediated_volatile_emissions_by_two_species_of_fungi_in_stored_grain/24855609 |
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| Summary: | <p>Our goals were to 1) isolate, and culture two fungal morphotypes, 2) characterize the volatile emissions from grain inoculated by each fungal morphotype (<em>Aspergillus flavus</em> or <em>Fusarium</em> spp.) compared to uninoculated and sanitized grain, and 3) understand how MVOCs from each morphotype affects mobility, attraction, and preference by <em>L. serricorne</em>. Headspace collection revealed that the <em>Fusarium</em>- and <em>A. flavus</em>-inoculated grain produced significantly different volatiles compared to sanitized grain or the positive control. Changes in MVOC emissions affected close-range foraging during an Ethovision assay, with a greater frequency of entering and spending time in a small zone with kernels inoculated with <em>A. flavus</em> compared to other treatments. In the release-recapture assay, MVOCs were found to be attractive to <em>L. serricorne</em> at a longer distances in commercial pitfall traps. While there was no preference shown among semiochemical stimuli in a still-air, four-way olfactometer, it is possible that methodological limitations prevented robust interpretation from this assay. Overall, our study suggests that MVOCs are important for close- and long-range orientation of <em>L.serricorne</em> during foraging, and that MVOCs may have the potential for inclusion in behaviorally-based tactics for this species.</p>
<div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: 4-way olfactometer assay.</p> <p>File Name: Sierra_2021_olfactometer_Ag_Data_commons.csv</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/en-us/microsoft-365/excel" target="_blank">https://www.microsoft.com/en-us/microsoft-365/excel</a> </p></li><br><li><p>Resource Title: Release-recapture Assay .</p> <p>File Name: sierra_release_recapture_exp_2021_fungal_volatiles_agdata_commons.csv</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/en-us/microsoft-365/excel" target="_blank">https://www.microsoft.com/en-us/microsoft-365/excel</a> </p></li><br><li><p>Resource Title: Ethovision Movement Assay .</p> <p>File Name: ethovision_sierra_2021_microbial_volatiles_agdatacommons.csv</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/en-us/microsoft-365/excel" target="_blank">https://www.microsoft.com/en-us/microsoft-365/excel</a> </p></li><br><li><p>Resource Title: Headspace volatile collection assay.</p> <p>File Name: headspace_compounds_sierra_2021_fungal_volatiles_final_agdatacommons.csv</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/en-us/microsoft-365/excel" target="_blank">https://www.microsoft.com/en-us/microsoft-365/excel</a> </p></li><br><li><p>Resource Title: Sequencing Data.</p> <p>File Name: sequencing_data.zip</p></li><br><li><p>Resource Title: File list.</p> <p>File Name: File_list_L_serricone_attraction_data.txt</p></li></ul> |
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