Data from: Overexpression of a Modified Plant Thionin Enhances Disease Resistance to Citrus Canker and Huanglongbing (HLB)
<p>Huanglongbing (HLB or citrus greening disease) caused by <em>Candidatus Liberibacter asiaticus</em> (Las) is a great threat to the US citrus industry. There are no proven strategies to eliminate HLB disease and no cultivar has been identified with strong HLB resistance. Citrus canker is also an economically important disease associated with a bacterial pathogen (<em>Xanthomonas citri</em>). In this study, we characterized endogenous citrus thionins and investigated their expression in different citrus tissues. Since no HLB-resistant citrus cultivars have been identified, we attempted to develop citrus resistant to both HLB and citrus canker through overexpression of a modified plant thionin. To improve effectiveness for disease resistance, we modified and synthesized the sequence encoding a plant thionin and cloned into the binary vector pBinPlus/ARS. The construct was then introduced into <em>Agrobacterium</em> strain EHA105 for citrus transformation. Transgenic Carrizo plants expressing the modified plant thionin were generated by <em>Agrobacterium</em>-mediated transformation. Successful transformation and transgene gene expression was confirmed by molecular analysis. Transgenic Carrizo plants expressing the modified thionin gene were challenged with <em>X. citri</em> 3213 at a range of concentrations, and a significant reduction in canker symptoms and a decrease in bacterial growth were demonstrated compared to nontransgenic plants. Furthermore, the transgenic citrus plants were challenged with HLB via graft inoculation. Our results showed significant Las titer reduction in roots of transgenic Carrizo compared with control plants and reduced scion Las titer 12 months after graft inoculation. These data provide promise for engineering citrus disease resistance against HLB and canker. </p><div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: Supplemental Tables S1 and S2, Las detection (download docx).</p> <p>File Name: Web Page, url: <a href="https://www.frontiersin.org/articles/file/downloadfile/205334_supplementary-materials_tables_1_docx/octet-stream/Table 1.docx/2/205334">https://www.frontiersin.org/articles/file/downloadfile/205334_supplementary-materials_tables_1_docx/octet-stream/Table 1.docx/2/205334</a> </p><p>Table S1. Root Las detection using qPCR and HLB symptom in control plants and transgenic plants expressing Mthionin nine months after graft inoculation.</p> <p>Table S2. Las detection from leaves and roots in control plants and transgenic plants expressing Mthionin twelve months after graft inoculation.</p></li></ul><p></p>
| Main Authors: | , , |
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| Format: | Dataset biblioteca |
| Published: |
2019
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| Subjects: | Crop and pasture production, Crop and pasture protection (incl. pests, diseases and weeds), Ecology, Genetics, Gene expression (incl. microarray and other genome-wide approaches), Genetics not elsewhere classified, Plant pathology, Plant biology not elsewhere classified, Environmental sciences, Huanglongbing, HLB, |
| Online Access: | https://figshare.com/articles/dataset/Data_from_Overexpression_of_a_Modified_Plant_Thionin_Enhances_Disease_Resistance_to_Citrus_Canker_and_Huanglongbing_HLB_/24853419 |
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| Summary: | <p>Huanglongbing (HLB or citrus greening disease) caused by <em>Candidatus Liberibacter asiaticus</em> (Las) is a great threat to the US citrus industry. There are no proven strategies to eliminate HLB disease and no cultivar has been identified with strong HLB resistance. Citrus canker is also an economically important disease associated with a bacterial pathogen (<em>Xanthomonas citri</em>). In this study, we characterized endogenous citrus thionins and investigated their expression in different citrus tissues. Since no HLB-resistant citrus cultivars have been identified, we attempted to develop citrus resistant to both HLB and citrus canker through overexpression of a modified plant thionin. To improve effectiveness for disease resistance, we modified and synthesized the sequence encoding a plant thionin and cloned into the binary vector pBinPlus/ARS. The construct was then introduced into <em>Agrobacterium</em> strain EHA105 for citrus transformation. Transgenic Carrizo plants expressing the modified plant thionin were generated by <em>Agrobacterium</em>-mediated transformation. Successful transformation and transgene gene expression was confirmed by molecular analysis. Transgenic Carrizo plants expressing the modified thionin gene were challenged with <em>X. citri</em> 3213 at a range of concentrations, and a significant reduction in canker symptoms and a decrease in bacterial growth were demonstrated compared to nontransgenic plants. Furthermore, the transgenic citrus plants were challenged with HLB via graft inoculation. Our results showed significant Las titer reduction in roots of transgenic Carrizo compared with control plants and reduced scion Las titer 12 months after graft inoculation. These data provide promise for engineering citrus disease resistance against HLB and canker. </p><div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: Supplemental Tables S1 and S2, Las detection (download docx).</p> <p>File Name: Web Page, url: <a href="https://www.frontiersin.org/articles/file/downloadfile/205334_supplementary-materials_tables_1_docx/octet-stream/Table 1.docx/2/205334">https://www.frontiersin.org/articles/file/downloadfile/205334_supplementary-materials_tables_1_docx/octet-stream/Table 1.docx/2/205334</a> </p><p>Table S1. Root Las detection using qPCR and HLB symptom in control plants and transgenic plants expressing Mthionin nine months after graft inoculation.</p>
<p>Table S2. Las detection from leaves and roots in control plants and transgenic plants expressing Mthionin twelve months after graft inoculation.</p></li></ul><p></p> |
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