Data from: Net release and uptake of xenometabolites across intestinal, hepatic, muscle, and renal tissue beds in healthy conscious pigs

<p>Data and supplemental figures for manuscript titled "Net release and uptake of xenometabolites across intestinal, hepatic, muscle, and renal tissue beds in healthy conscious pigs." We used a catheterized conscious pig model to estimate inter-organ flux of xenometabolites, derivatives, and bile acids using LC/MS. Female pigs (n=12; 2-3 months old; 25.6 ± 2.2 kg) had surgically-implanted catheters across portal-drained viscera (PDV), splanchnic area (SPL), liver, kidney, and hindquarter muscle. Overnight fasted arterial and venous plasma was collected simultaneously in a conscious state and stored at -80°C. Thawed samples were analyzed by liquid chromatography-mass spectrometry. Plasma flow was determined with para-aminohippuric acid dilution technology and used to calculate net organ balance for each metabolite. This repository contains the LC/MS data and supplemental figures for the submitted manuscript. </p><div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: Organ Net Balance Data for Mercer et al..</p> <p>File Name: Mercer et al LCMS XM Organ Flux Data.xlsx</p><p>Resource Description: Calculated net balances for LC/MS data from Mercer et al. </p></li><br><li><p>Resource Title: LC/MS data for Mercer et al..</p> <p>File Name: Mercer et al LCMS XM raw data.xlsx</p><p>Resource Description: LC/MS peak area data for xenometabolites in Mercer et al.</p></li><br><li><p>Resource Title: Mercer et al Supplemental Figure 2.</p> <p>File Name: Mercer et al Fig S2.pdf</p><p>Resource Description: Supplemental Figure 2 for Mercer et al manuscript: Absolute differences of xenometabolites from catheter ports in conscious overnight-fasted female pigs (n=12). Sampling sites included arterial, venous, renal, portal, and hepatic placed catheters. </p></li><br><li><p>Resource Title: Mercer et al Supplemental Figure 1.</p> <p>File Name: Mercer et al Fig S1.pdf</p><p>Resource Description: Supplemental Figure 1 for Mercer et al manuscript: Supplemental Figure 1: Inter-organ flux of all xenometablites in conscious overnight-fasted female pigs (n=12). A negative flux reflects net uptake (flux < 0; indicated by bottom arrow) by the organ, whereas a positive flux reflects a net release (flux > 0; indicated by top arrow) by the organ. Plasma flow measurements were determined with the para-aminohippuric acid dilution method, and were used along with metabolite concentrations to calculate flux (see Methods). R at top of arrow indicates ‘Release’, while U indicates ‘Uptake’. </p></li><br><li><p>Resource Title: Supplemental Table 1 for Mercer et al..</p> <p>File Name: Mercer et al Table S1.xlsx</p><p>Resource Description: Mercer et al Supplemental Table 1: Raw differences of identified xenometabolites from port samples in conscious adult female pigs (n=10-12).</p></li></ul><p></p>

Bibliographic Details

Main Authors:	Kelly E. Mercer (14011008), Gabriella A. M. Ten Have (17482161), Lindsay Pack (596692), Renny Lan (17482164), Nicolaas E. P. Deutz (17482167), Sean H. Adams (17481399), Brian D. Piccolo (17482170)
Format:	Dataset biblioteca
Published:	2020
Subjects:	Food sciences, Food nutritional balance, metabolomics, xenometabolite, pig model, tissue flux, inter organ balance, NP107, data.gov, ARS,
Online Access:	https://figshare.com/articles/dataset/Data_from_Net_release_and_uptake_of_xenometabolites_across_intestinal_hepatic_muscle_and_renal_tissue_beds_in_healthy_conscious_pigs/24665562
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