Somatic embryogenesis of cocoa from floral parts.
Somatic embryogenesis from floral buds was tested on 25 cocoa genotypes under defined culture conditions, revealing genotypic variations. Histological monitoring of somatic embryo ontogenesis revealed that the somatic embryos were of multicellular origin. The right conditions for the selection and maintenance of meristematic cells (from which somatic embryos are derived) and embryogenic cells were sought by culturing in a liquid medium using a temporary immersion system (TIS). A friable embryogeniccallus was obtained. A study of zygotic embryogenesis was carried out with a view to improving the later stages of somatic embryogenesis. Zygotic embryogenesis was characterized by a period of embryo growth, followed by accumulation of starch and proteinreserves, during which slow and moderate desiccation occurred. By adding a growth phase to the somatic embryogenesis protocol, and defining a maturation medium containing abscisic acid, embryo maturation was improved.
Main Authors: | , , |
---|---|
Format: | Texto biblioteca |
Language: | ng |
Published: |
©199
|
Subjects: | Coco., in vitro culture, somatic embryogenesis, stimulant plants, tissue culture, tropical crops, Somatic embryogenesis, Tissue culture, Tropical crops, |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
cat-fedepalma-23600 |
---|---|
record_format |
koha |
spelling |
cat-fedepalma-236002022-08-05T00:10:37ZSomatic embryogenesis of cocoa from floral parts. Alemanno, L. 42706, autor. aut Berthouly, M. 42707. Michaux F., Nicole 42708. text©1996ng Somatic embryogenesis from floral buds was tested on 25 cocoa genotypes under defined culture conditions, revealing genotypic variations. Histological monitoring of somatic embryo ontogenesis revealed that the somatic embryos were of multicellular origin. The right conditions for the selection and maintenance of meristematic cells (from which somatic embryos are derived) and embryogenic cells were sought by culturing in a liquid medium using a temporary immersion system (TIS). A friable embryogeniccallus was obtained. A study of zygotic embryogenesis was carried out with a view to improving the later stages of somatic embryogenesis. Zygotic embryogenesis was characterized by a period of embryo growth, followed by accumulation of starch and proteinreserves, during which slow and moderate desiccation occurred. By adding a growth phase to the somatic embryogenesis protocol, and defining a maturation medium containing abscisic acid, embryo maturation was improved.Incluye 11 referencias bibliográficas.Somatic embryogenesis from floral buds was tested on 25 cocoa genotypes under defined culture conditions, revealing genotypic variations. Histological monitoring of somatic embryo ontogenesis revealed that the somatic embryos were of multicellular origin. The right conditions for the selection and maintenance of meristematic cells (from which somatic embryos are derived) and embryogenic cells were sought by culturing in a liquid medium using a temporary immersion system (TIS). A friable embryogeniccallus was obtained. A study of zygotic embryogenesis was carried out with a view to improving the later stages of somatic embryogenesis. Zygotic embryogenesis was characterized by a period of embryo growth, followed by accumulation of starch and proteinreserves, during which slow and moderate desiccation occurred. By adding a growth phase to the somatic embryogenesis protocol, and defining a maturation medium containing abscisic acid, embryo maturation was improved.Coco.in vitro culturesomatic embryogenesisstimulant plantstissue culturetropical cropsSomatic embryogenesisTissue cultureTropical crops |
institution |
FEDEPALMA |
collection |
Koha |
country |
Colombia |
countrycode |
CO |
component |
Bibliográfico |
access |
En linea |
databasecode |
cat-fedepalma |
tag |
biblioteca |
region |
America del Sur |
libraryname |
Centro de Información y Documentación Palmero |
language |
ng |
topic |
Coco. in vitro culture somatic embryogenesis stimulant plants tissue culture tropical crops Somatic embryogenesis Tissue culture Tropical crops Coco. in vitro culture somatic embryogenesis stimulant plants tissue culture tropical crops Somatic embryogenesis Tissue culture Tropical crops |
spellingShingle |
Coco. in vitro culture somatic embryogenesis stimulant plants tissue culture tropical crops Somatic embryogenesis Tissue culture Tropical crops Coco. in vitro culture somatic embryogenesis stimulant plants tissue culture tropical crops Somatic embryogenesis Tissue culture Tropical crops Alemanno, L. 42706, autor. aut Berthouly, M. 42707. Michaux F., Nicole 42708. Somatic embryogenesis of cocoa from floral parts. |
description |
Somatic embryogenesis from floral buds was tested on 25 cocoa genotypes under defined culture conditions, revealing genotypic variations. Histological monitoring of somatic embryo ontogenesis revealed that the somatic embryos were of multicellular origin. The right conditions for the selection and maintenance of meristematic cells (from which somatic embryos are derived) and embryogenic cells were sought by culturing in a liquid medium using a temporary immersion system (TIS). A friable embryogeniccallus was obtained. A study of zygotic embryogenesis was carried out with a view to improving the later stages of somatic embryogenesis. Zygotic embryogenesis was characterized by a period of embryo growth, followed by accumulation of starch and proteinreserves, during which slow and moderate desiccation occurred. By adding a growth phase to the somatic embryogenesis protocol, and defining a maturation medium containing abscisic acid, embryo maturation was improved. |
format |
Texto |
topic_facet |
Coco. in vitro culture somatic embryogenesis stimulant plants tissue culture tropical crops Somatic embryogenesis Tissue culture Tropical crops |
author |
Alemanno, L. 42706, autor. aut Berthouly, M. 42707. Michaux F., Nicole 42708. |
author_facet |
Alemanno, L. 42706, autor. aut Berthouly, M. 42707. Michaux F., Nicole 42708. |
author_sort |
Alemanno, L. 42706, autor. aut |
title |
Somatic embryogenesis of cocoa from floral parts. |
title_short |
Somatic embryogenesis of cocoa from floral parts. |
title_full |
Somatic embryogenesis of cocoa from floral parts. |
title_fullStr |
Somatic embryogenesis of cocoa from floral parts. |
title_full_unstemmed |
Somatic embryogenesis of cocoa from floral parts. |
title_sort |
somatic embryogenesis of cocoa from floral parts. |
publishDate |
©199 |
work_keys_str_mv |
AT alemannol42706autoraut somaticembryogenesisofcocoafromfloralparts AT berthoulym42707 somaticembryogenesisofcocoafromfloralparts AT michauxfnicole42708 somaticembryogenesisofcocoafromfloralparts |
_version_ |
1756213664566738944 |