Nonradioactive Labeling and Detection of Biomolecules [electronic resource] /

1 General Aspects of Nonradioactive Labeling and Detection -- 1.1 Introduction -- 1.2 The Concept of Nonradioactive Bioanalytics. -- 1.3 Direct and Indirect Labeling and Detection Systems -- 1.4 Labeling Methods -- 1.5 Detection Methods -- 1.6 Guide of the Use of Information in this Book -- I Standard Nonradioactive Labeling and Detection Systems -- 2 Overview of Nonradioactive Labeling Systems -- 3 The Digoxigenin: Anti-Digoxigenin (DIG) System -- 4 The Biotin System -- 5 In Vivo Labeling of DNA Probes with 5-BrdU -- 6 The Sulfone System -- 7 Colloidal Gold as a Marker in Molecular Biology: The Use of Ultra-Small Gold Probes -- 8 Direct Peroxidase Labeling of Hybridization Probes and Chemiluminescence Detection -- 9 A Highly Sensitive Method for Detecting Peroxidase in In Situ Hybridization or Immunohistochemical Assays -- 10 The SNAP System -- II Specialized Nonradioactive Detection Systems -- 11 Overview of Colorimetric, Chemiluminometric, and Fluorimetric Detection Systems -- 12 Colorimetric Systems -- 13 Luminescence Systems -- 14 Fluorescence Systems -- III Enhanced Systems -- 15 Overview of Amplification Systems -- 16 Enhanced Signal Generation by Target Amplification -- 17 Signal Amplification System: Substrate Cascade -- IV Application Formats -- 18 Overview of Application Formats -- 19 Blot Formats: Nucleic Acids -- 20 Blot Formats: Proteins and Glycoproteins -- 21 In Situ Formats -- 22 Quantitative Formats -- Appendix I Suppliers of Reagents -- Appendix II Suppliers of Equipment.

Bibliographic Details

Main Authors:	Kessler, Christoph. editor., SpringerLink (Online service)
Format:	Texto biblioteca
Language:	eng
Published:	Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 1992
Subjects:	Life sciences., Biochemistry., Cell biology., Life Sciences., Cell Biology., Biochemistry, general.,
Online Access:	http://dx.doi.org/10.1007/978-3-662-00144-8
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