Mass-rearing conditions do not always reduce genetic diversity the case of the mexican fruit fly, Anastrepha ludens (Diptera: Tephritidae)
The application of the sterile insect technique (SIT) requires the adaptation of insects to mass-rearing conditions. It is generally accepted that this adaptation may include a reduction in genetic diversity and an associated loss of desirable characteristics for the effective performance of sterile insects in the field. Here, we compare the genetic diversity of two mass-reared strains of the Mexican fruit fly, Anastrepha ludens, and a wild (WIL) population collected near Tapachula, Mexico, using seven DNA microsatellites as molecular genetic markers. The mass-reared strains were a bisexual laboratory strain (LAB) with approximately 130 generations under mass-rearing and a genetic sexing strain, Tapachula-7 (TA7), also under mass-rearing for 100 generations. Our results revealed an overall low level of genetic differentiation (approximately 15%) among the three strains, with the LAB and WIL populations being genetically most similar and TA7 most genetically differentiated. Although there were some differences in allele frequencies between strains, our results show that overall, the adaptation to mass-rearing conditions did not reduce genetic variability compared to the wild sample in terms of heterozygosity or allelic richness, nor did it appear to alter the level of inbreeding with respect to the wild populations. These results are contrary to the general idea that mass-rearing always results in a reduction in genetic diversity. Overall, our findings can contribute to a better understanding of the impact that adaptation to mass-rearing conditions may have on the genetic make-up of strains.
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| Subjects: | Anastrepha ludens, Moscas de la fruta, Técnica del insecto estéril, Variación genética, Control de plagas, Artfrosur, |
| Online Access: | https://doi.org/10.3390/insects15010056 |
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KOHA-OAI-ECOSUR:644572024-08-26T11:27:15ZMass-rearing conditions do not always reduce genetic diversity the case of the mexican fruit fly, Anastrepha ludens (Diptera: Tephritidae) Ruiz Montoya, Lorena 1964- Doctora autora 5452 Sánchez Rosario, Mayren Doctora autora 13102 López Gómez, Emiliano autor García Bautista, Maricela autora 8676 Canedo Texón, Anahí autora Haymer, David autor Liedo Fernández, Pablo Doctor autor 67 textengThe application of the sterile insect technique (SIT) requires the adaptation of insects to mass-rearing conditions. It is generally accepted that this adaptation may include a reduction in genetic diversity and an associated loss of desirable characteristics for the effective performance of sterile insects in the field. Here, we compare the genetic diversity of two mass-reared strains of the Mexican fruit fly, Anastrepha ludens, and a wild (WIL) population collected near Tapachula, Mexico, using seven DNA microsatellites as molecular genetic markers. The mass-reared strains were a bisexual laboratory strain (LAB) with approximately 130 generations under mass-rearing and a genetic sexing strain, Tapachula-7 (TA7), also under mass-rearing for 100 generations. Our results revealed an overall low level of genetic differentiation (approximately 15%) among the three strains, with the LAB and WIL populations being genetically most similar and TA7 most genetically differentiated. Although there were some differences in allele frequencies between strains, our results show that overall, the adaptation to mass-rearing conditions did not reduce genetic variability compared to the wild sample in terms of heterozygosity or allelic richness, nor did it appear to alter the level of inbreeding with respect to the wild populations. These results are contrary to the general idea that mass-rearing always results in a reduction in genetic diversity. Overall, our findings can contribute to a better understanding of the impact that adaptation to mass-rearing conditions may have on the genetic make-up of strains.The application of the sterile insect technique (SIT) requires the adaptation of insects to mass-rearing conditions. It is generally accepted that this adaptation may include a reduction in genetic diversity and an associated loss of desirable characteristics for the effective performance of sterile insects in the field. Here, we compare the genetic diversity of two mass-reared strains of the Mexican fruit fly, Anastrepha ludens, and a wild (WIL) population collected near Tapachula, Mexico, using seven DNA microsatellites as molecular genetic markers. The mass-reared strains were a bisexual laboratory strain (LAB) with approximately 130 generations under mass-rearing and a genetic sexing strain, Tapachula-7 (TA7), also under mass-rearing for 100 generations. Our results revealed an overall low level of genetic differentiation (approximately 15%) among the three strains, with the LAB and WIL populations being genetically most similar and TA7 most genetically differentiated. Although there were some differences in allele frequencies between strains, our results show that overall, the adaptation to mass-rearing conditions did not reduce genetic variability compared to the wild sample in terms of heterozygosity or allelic richness, nor did it appear to alter the level of inbreeding with respect to the wild populations. These results are contrary to the general idea that mass-rearing always results in a reduction in genetic diversity. Overall, our findings can contribute to a better understanding of the impact that adaptation to mass-rearing conditions may have on the genetic make-up of strains.Anastrepha ludensMoscas de la frutaTécnica del insecto estérilVariación genéticaControl de plagasArtfrosurInsectshttps://doi.org/10.3390/insects15010056Acceso en línea sin restricciones |
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Anastrepha ludens Moscas de la fruta Técnica del insecto estéril Variación genética Control de plagas Artfrosur Anastrepha ludens Moscas de la fruta Técnica del insecto estéril Variación genética Control de plagas Artfrosur |
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Anastrepha ludens Moscas de la fruta Técnica del insecto estéril Variación genética Control de plagas Artfrosur Anastrepha ludens Moscas de la fruta Técnica del insecto estéril Variación genética Control de plagas Artfrosur Ruiz Montoya, Lorena 1964- Doctora autora 5452 Sánchez Rosario, Mayren Doctora autora 13102 López Gómez, Emiliano autor García Bautista, Maricela autora 8676 Canedo Texón, Anahí autora Haymer, David autor Liedo Fernández, Pablo Doctor autor 67 Mass-rearing conditions do not always reduce genetic diversity the case of the mexican fruit fly, Anastrepha ludens (Diptera: Tephritidae) |
| description |
The application of the sterile insect technique (SIT) requires the adaptation of insects to mass-rearing conditions. It is generally accepted that this adaptation may include a reduction in genetic diversity and an associated loss of desirable characteristics for the effective performance of sterile insects in the field. Here, we compare the genetic diversity of two mass-reared strains of the Mexican fruit fly, Anastrepha ludens, and a wild (WIL) population collected near Tapachula, Mexico, using seven DNA microsatellites as molecular genetic markers. The mass-reared strains were a bisexual laboratory strain (LAB) with approximately 130 generations under mass-rearing and a genetic sexing strain, Tapachula-7 (TA7), also under mass-rearing for 100 generations. Our results revealed an overall low level of genetic differentiation (approximately 15%) among the three strains, with the LAB and WIL populations being genetically most similar and TA7 most genetically differentiated. Although there were some differences in allele frequencies between strains, our results show that overall, the adaptation to mass-rearing conditions did not reduce genetic variability compared to the wild sample in terms of heterozygosity or allelic richness, nor did it appear to alter the level of inbreeding with respect to the wild populations. These results are contrary to the general idea that mass-rearing always results in a reduction in genetic diversity. Overall, our findings can contribute to a better understanding of the impact that adaptation to mass-rearing conditions may have on the genetic make-up of strains. |
| format |
Texto |
| topic_facet |
Anastrepha ludens Moscas de la fruta Técnica del insecto estéril Variación genética Control de plagas Artfrosur |
| author |
Ruiz Montoya, Lorena 1964- Doctora autora 5452 Sánchez Rosario, Mayren Doctora autora 13102 López Gómez, Emiliano autor García Bautista, Maricela autora 8676 Canedo Texón, Anahí autora Haymer, David autor Liedo Fernández, Pablo Doctor autor 67 |
| author_facet |
Ruiz Montoya, Lorena 1964- Doctora autora 5452 Sánchez Rosario, Mayren Doctora autora 13102 López Gómez, Emiliano autor García Bautista, Maricela autora 8676 Canedo Texón, Anahí autora Haymer, David autor Liedo Fernández, Pablo Doctor autor 67 |
| author_sort |
Ruiz Montoya, Lorena 1964- Doctora autora 5452 |
| title |
Mass-rearing conditions do not always reduce genetic diversity the case of the mexican fruit fly, Anastrepha ludens (Diptera: Tephritidae) |
| title_short |
Mass-rearing conditions do not always reduce genetic diversity the case of the mexican fruit fly, Anastrepha ludens (Diptera: Tephritidae) |
| title_full |
Mass-rearing conditions do not always reduce genetic diversity the case of the mexican fruit fly, Anastrepha ludens (Diptera: Tephritidae) |
| title_fullStr |
Mass-rearing conditions do not always reduce genetic diversity the case of the mexican fruit fly, Anastrepha ludens (Diptera: Tephritidae) |
| title_full_unstemmed |
Mass-rearing conditions do not always reduce genetic diversity the case of the mexican fruit fly, Anastrepha ludens (Diptera: Tephritidae) |
| title_sort |
mass-rearing conditions do not always reduce genetic diversity the case of the mexican fruit fly, anastrepha ludens (diptera: tephritidae) |
| url |
https://doi.org/10.3390/insects15010056 |
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