Broad-spectrum antifungal, biosurfactants and bioemulsifier activity of bacillus subtilis subsp. spizizenii—A potential biocontrol and bioremediation agent in agriculture
In this study, the antifungal, biosurfactant and bioemulsifying activity of the lipopeptides produced by the marine bacterium Bacillus subtilis subsp. spizizenii MC6B-22 is presented. The kinetics showed that at 84 h, the highest yield of lipopeptides (556 mg/mL) with antifungal, biosurfactant, bioemulsifying and hemolytic activity was detected, finding a relationship with the sporulation of the bacteria. Based on the hemolytic activity, bio-guided purification methods were used to obtain the lipopeptide. By TLC, HPLC and MALDI-TOF, the mycosubtilin was identified as the main lipopeptide, and it was further confirmed by NRPS gene clusters prediction based on the strain’s genome sequence, in addition to other genes related to antimicrobial activity. The lipopeptide showed a broad-spectrum activity against ten phytopathogens of tropical crops at a minimum inhibitory concentration of 400 to 25 µg/mL and with a fungicidal mode of action. In addition, it exhibited that biosurfactant and bioemulsifying activities remain stable over a wide range of salinity and pH and it can emulsify different hydrophobic substrates. These results demonstrate the potential of the MC6B-22 strain as a biocontrol agent for agriculture and its application in bioremediation and other biotechnological fields.
Main Authors: | , , , , , , |
---|---|
Format: | Texto biblioteca |
Language: | eng |
Subjects: | Bacillus subtilis, Lipopéptidos, Agentes antifúngicos, Biorremediación, |
Online Access: | https://doi.org/10.3390/plants12061374 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
KOHA-OAI-ECOSUR:63713 |
---|---|
record_format |
koha |
spelling |
KOHA-OAI-ECOSUR:637132024-03-12T12:33:23ZBroad-spectrum antifungal, biosurfactants and bioemulsifier activity of bacillus subtilis subsp. spizizenii—A potential biocontrol and bioremediation agent in agriculture Guillén Navarro, Griselda Karina Doctora autora 7945 López Gutiérrez, Tomás autor García Fajardo, Verónica autora Gómez Cornelio, Sergio Alberto Doctor autor 12785 Zarza Franco, Guadalupe Eugenia Doctora autora 21164 De la Rosa García, Susana del Carmen autora 14525 Chan Bacab, Manuel autor textengIn this study, the antifungal, biosurfactant and bioemulsifying activity of the lipopeptides produced by the marine bacterium Bacillus subtilis subsp. spizizenii MC6B-22 is presented. The kinetics showed that at 84 h, the highest yield of lipopeptides (556 mg/mL) with antifungal, biosurfactant, bioemulsifying and hemolytic activity was detected, finding a relationship with the sporulation of the bacteria. Based on the hemolytic activity, bio-guided purification methods were used to obtain the lipopeptide. By TLC, HPLC and MALDI-TOF, the mycosubtilin was identified as the main lipopeptide, and it was further confirmed by NRPS gene clusters prediction based on the strain’s genome sequence, in addition to other genes related to antimicrobial activity. The lipopeptide showed a broad-spectrum activity against ten phytopathogens of tropical crops at a minimum inhibitory concentration of 400 to 25 µg/mL and with a fungicidal mode of action. In addition, it exhibited that biosurfactant and bioemulsifying activities remain stable over a wide range of salinity and pH and it can emulsify different hydrophobic substrates. These results demonstrate the potential of the MC6B-22 strain as a biocontrol agent for agriculture and its application in bioremediation and other biotechnological fields.In this study, the antifungal, biosurfactant and bioemulsifying activity of the lipopeptides produced by the marine bacterium Bacillus subtilis subsp. spizizenii MC6B-22 is presented. The kinetics showed that at 84 h, the highest yield of lipopeptides (556 mg/mL) with antifungal, biosurfactant, bioemulsifying and hemolytic activity was detected, finding a relationship with the sporulation of the bacteria. Based on the hemolytic activity, bio-guided purification methods were used to obtain the lipopeptide. By TLC, HPLC and MALDI-TOF, the mycosubtilin was identified as the main lipopeptide, and it was further confirmed by NRPS gene clusters prediction based on the strain’s genome sequence, in addition to other genes related to antimicrobial activity. The lipopeptide showed a broad-spectrum activity against ten phytopathogens of tropical crops at a minimum inhibitory concentration of 400 to 25 µg/mL and with a fungicidal mode of action. In addition, it exhibited that biosurfactant and bioemulsifying activities remain stable over a wide range of salinity and pH and it can emulsify different hydrophobic substrates. These results demonstrate the potential of the MC6B-22 strain as a biocontrol agent for agriculture and its application in bioremediation and other biotechnological fields.Bacillus subtilisLipopéptidosAgentes antifúngicosBiorremediaciónPlantshttps://doi.org/10.3390/plants12061374Acceso en línea sin restricciones |
institution |
ECOSUR |
collection |
Koha |
country |
México |
countrycode |
MX |
component |
Bibliográfico |
access |
En linea En linea |
databasecode |
cat-ecosur |
tag |
biblioteca |
region |
America del Norte |
libraryname |
Sistema de Información Bibliotecario de ECOSUR (SIBE) |
language |
eng |
topic |
Bacillus subtilis Lipopéptidos Agentes antifúngicos Biorremediación Bacillus subtilis Lipopéptidos Agentes antifúngicos Biorremediación |
spellingShingle |
Bacillus subtilis Lipopéptidos Agentes antifúngicos Biorremediación Bacillus subtilis Lipopéptidos Agentes antifúngicos Biorremediación Guillén Navarro, Griselda Karina Doctora autora 7945 López Gutiérrez, Tomás autor García Fajardo, Verónica autora Gómez Cornelio, Sergio Alberto Doctor autor 12785 Zarza Franco, Guadalupe Eugenia Doctora autora 21164 De la Rosa García, Susana del Carmen autora 14525 Chan Bacab, Manuel autor Broad-spectrum antifungal, biosurfactants and bioemulsifier activity of bacillus subtilis subsp. spizizenii—A potential biocontrol and bioremediation agent in agriculture |
description |
In this study, the antifungal, biosurfactant and bioemulsifying activity of the lipopeptides produced by the marine bacterium Bacillus subtilis subsp. spizizenii MC6B-22 is presented. The kinetics showed that at 84 h, the highest yield of lipopeptides (556 mg/mL) with antifungal, biosurfactant, bioemulsifying and hemolytic activity was detected, finding a relationship with the sporulation of the bacteria. Based on the hemolytic activity, bio-guided purification methods were used to obtain the lipopeptide. By TLC, HPLC and MALDI-TOF, the mycosubtilin was identified as the main lipopeptide, and it was further confirmed by NRPS gene clusters prediction based on the strain’s genome sequence, in addition to other genes related to antimicrobial activity. The lipopeptide showed a broad-spectrum activity against ten phytopathogens of tropical crops at a minimum inhibitory concentration of 400 to 25 µg/mL and with a fungicidal mode of action. In addition, it exhibited that biosurfactant and bioemulsifying activities remain stable over a wide range of salinity and pH and it can emulsify different hydrophobic substrates. These results demonstrate the potential of the MC6B-22 strain as a biocontrol agent for agriculture and its application in bioremediation and other biotechnological fields. |
format |
Texto |
topic_facet |
Bacillus subtilis Lipopéptidos Agentes antifúngicos Biorremediación |
author |
Guillén Navarro, Griselda Karina Doctora autora 7945 López Gutiérrez, Tomás autor García Fajardo, Verónica autora Gómez Cornelio, Sergio Alberto Doctor autor 12785 Zarza Franco, Guadalupe Eugenia Doctora autora 21164 De la Rosa García, Susana del Carmen autora 14525 Chan Bacab, Manuel autor |
author_facet |
Guillén Navarro, Griselda Karina Doctora autora 7945 López Gutiérrez, Tomás autor García Fajardo, Verónica autora Gómez Cornelio, Sergio Alberto Doctor autor 12785 Zarza Franco, Guadalupe Eugenia Doctora autora 21164 De la Rosa García, Susana del Carmen autora 14525 Chan Bacab, Manuel autor |
author_sort |
Guillén Navarro, Griselda Karina Doctora autora 7945 |
title |
Broad-spectrum antifungal, biosurfactants and bioemulsifier activity of bacillus subtilis subsp. spizizenii—A potential biocontrol and bioremediation agent in agriculture |
title_short |
Broad-spectrum antifungal, biosurfactants and bioemulsifier activity of bacillus subtilis subsp. spizizenii—A potential biocontrol and bioremediation agent in agriculture |
title_full |
Broad-spectrum antifungal, biosurfactants and bioemulsifier activity of bacillus subtilis subsp. spizizenii—A potential biocontrol and bioremediation agent in agriculture |
title_fullStr |
Broad-spectrum antifungal, biosurfactants and bioemulsifier activity of bacillus subtilis subsp. spizizenii—A potential biocontrol and bioremediation agent in agriculture |
title_full_unstemmed |
Broad-spectrum antifungal, biosurfactants and bioemulsifier activity of bacillus subtilis subsp. spizizenii—A potential biocontrol and bioremediation agent in agriculture |
title_sort |
broad-spectrum antifungal, biosurfactants and bioemulsifier activity of bacillus subtilis subsp. spizizenii—a potential biocontrol and bioremediation agent in agriculture |
url |
https://doi.org/10.3390/plants12061374 |
work_keys_str_mv |
AT guillennavarrogriseldakarinadoctoraautora7945 broadspectrumantifungalbiosurfactantsandbioemulsifieractivityofbacillussubtilissubspspizizeniiapotentialbiocontrolandbioremediationagentinagriculture AT lopezgutierreztomasautor broadspectrumantifungalbiosurfactantsandbioemulsifieractivityofbacillussubtilissubspspizizeniiapotentialbiocontrolandbioremediationagentinagriculture AT garciafajardoveronicaautora broadspectrumantifungalbiosurfactantsandbioemulsifieractivityofbacillussubtilissubspspizizeniiapotentialbiocontrolandbioremediationagentinagriculture AT gomezcorneliosergioalbertodoctorautor12785 broadspectrumantifungalbiosurfactantsandbioemulsifieractivityofbacillussubtilissubspspizizeniiapotentialbiocontrolandbioremediationagentinagriculture AT zarzafrancoguadalupeeugeniadoctoraautora21164 broadspectrumantifungalbiosurfactantsandbioemulsifieractivityofbacillussubtilissubspspizizeniiapotentialbiocontrolandbioremediationagentinagriculture AT delarosagarciasusanadelcarmenautora14525 broadspectrumantifungalbiosurfactantsandbioemulsifieractivityofbacillussubtilissubspspizizeniiapotentialbiocontrolandbioremediationagentinagriculture AT chanbacabmanuelautor broadspectrumantifungalbiosurfactantsandbioemulsifieractivityofbacillussubtilissubspspizizeniiapotentialbiocontrolandbioremediationagentinagriculture |
_version_ |
1794792455288127488 |