The gut microbiome analysis of Anastrepha obliqua reveals inter‑kingdom diversity bacteria, fungi, and archaea
The fruit fly Anastrepha obliqua is an economically important pest. The sterile insect technique to control it involves mass production and release of sterile flies to reduce the reproduction of the wild population. As noted in different Tephritidae, the performance of sterile males may be afected by the assimilation of nutrients under mass-rearing conditions. In the wild, the fly's life cycle suggests the acquisition of diferent organisms that could modulate its fitness and physiology. For A. obliqua, there is no information regarding microorganisms other than bacteria. This study analyzed bacteria, fungal, and archaea communities in the A. obliqua gut through denaturing gradient gel electrophoresis (DGGE) profles of 16S (using a diferent set of primers for bacteria and archaea) and 18S ribosomal DNA markers. We found that wild flies presented higher microbial diversity related to fructose assimilation than laboratory species, suggesting that microorganisms have led to a specialized metabolism to process nutrients associated with an artifcial diet. We identifed species that have not been previously described in this fruit fly, especially actinobacteria and archaea, by employing diferent primer sets aimed at the same molecular marker but targeting diverse hypervariable regions of 16S rDNA. The possibility that Archaea affect fly fitness should not be ignored. This report on the intestinal microbial (bacteria, archaea, and fungi) composition of A. obliqua contributes to our understanding of the role of microorganisms in the development and physiology of the flies.
Main Authors: | Amores, G. R. autor/a, Zepeda Ramos, G. autor/a, García Fajardo, L. V. autor/a, Hernández Ortiz, Emilio Doctor autor 12449, Guillén Navarro, K. autor/a |
---|---|
Format: | Texto biblioteca |
Language: | eng |
Subjects: | Anastrepha obliqua, Moscas de la fruta, Diversidad microbiana, Técnica del insecto estéril, |
Online Access: | https://doi.org/10.1007/s00203-022-03207-y |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Archaea, the unseen kingdom in the gut microbiome of Anastrepha obliqua
by: Amores, G. R. autor/a, et al. -
Optimum dosage for irradiating anastrepha obliqua pupae to obtain highly competitive sterile adults
by: Toledo, Jorge Doctor autor 5445 -
Pre-release diet effect on field survival and dispersal of Anastrepha ludens and Anastrepha obliqua (Diptera: Tephritidae)
by: Utgés, M. E., et al. -
Midgut protease activity during larval development of Anastrepha obliqua (Diptera: Tephritidae) fed with natural and artificial diet
by: Rivera Ciprian, José Pedro Maestro 13945, et al. -
Effect of post-teneral diets on the performance of sterile Anastrepha ludens and Anastrepha obliqua fruit flies
by: Liedo Fernández, Pablo Doctor 67, et al.