Volatile organic compounds produced by cacao endophytic bacteria and their inhibitory activity on moniliophthora roreri
Several studies have reported that bacteria produce anti-fungal volatiles. We identifed the organic volatile compounds produced by six cacao endophytic bacteria (CEB) strains (Bacillus amyloliquefaciens CFFSUR-B35, Bacillus megaterium CFFSUR-B32, Bacillus muralis CFFSUR-B39, Bacillus pumilus CFFSUR-B34, Bacillus subtilis CFFSUR-B31, and Novo sphingobium lindaniclasticum CFFSUR-B36). We evaluated their inhibitory efect on mycelium growth and spore germi nation of the phytopathogenic fungus Moniliophtora roreri. The volatiles produced by these six CEB, were collected and identifed by SPME and GC–MS. Moreover, the inhibitory efect of fve synthetic volatile organic compounds, individually and in mixtures (dimethyl disulfde, 2–5 dimethyl pyrazine, α-pinene, 2-heptanone and 2-ethyl hexanol) on M. roreri myce lium growth and spore germination was evaluated. All strains examined produced volatiles in diferent amounts; 13 to 10 compounds were identifed, including sulfde, alcohol benzene derivate, pyrazine, ketone, nitrogen and terpene compounds. The B. subtilis CFFSUR-B31 strain produced the largest number of volatiles, while B. pumilus CFFSUR-B34 produced the fewest and the lowest amounts. The volatile organic compounds produced by B. pumilus CFFSUR-B34, B. muralis CFFSUR B39 and N. lindaniclasticum CFFSUR-B36 inhibited M. roreri mycelium growth by more than 35%, sporulation by more than 81% and spore germination by more than 74%. However, when synthetic compounds were evaluated individually and in mixtures, 2-ethyl hexanol at 100,000 ppm (20 mg/flter paper disc) inhibited M. roreri mycelium growth by 100%, followed by organic volatile compound mixtures C (dimethyl disulfde, 2,5-dimethyl pyrazine, α-pinene, 2-ethyl-hexanol, 2-Heptanone) and D (only the top four) at 100,000 ppm (4 and 5 mg/flter paper disc) which inhibited spore germination by 97 and 89%, respectively.
Main Authors: | , , , , |
---|---|
Format: | Texto biblioteca |
Language: | eng |
Subjects: | Moniliophthora roreri, Hongos fitopatógenos, Theobroma cacao, Compuestos orgánicos volátiles, Hongos endófitos, Manejo de enfermedades y plagas, |
Online Access: | https://doi.org/10.1007/s00284-021-02696-2 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
KOHA-OAI-ECOSUR:62042 |
---|---|
record_format |
koha |
institution |
ECOSUR |
collection |
Koha |
country |
México |
countrycode |
MX |
component |
Bibliográfico |
access |
En linea En linea |
databasecode |
cat-ecosur |
tag |
biblioteca |
region |
America del Norte |
libraryname |
Sistema de Información Bibliotecario de ECOSUR (SIBE) |
language |
eng |
topic |
Moniliophthora roreri Hongos fitopatógenos Theobroma cacao Compuestos orgánicos volátiles Hongos endófitos Manejo de enfermedades y plagas Moniliophthora roreri Hongos fitopatógenos Theobroma cacao Compuestos orgánicos volátiles Hongos endófitos Manejo de enfermedades y plagas |
spellingShingle |
Moniliophthora roreri Hongos fitopatógenos Theobroma cacao Compuestos orgánicos volátiles Hongos endófitos Manejo de enfermedades y plagas Moniliophthora roreri Hongos fitopatógenos Theobroma cacao Compuestos orgánicos volátiles Hongos endófitos Manejo de enfermedades y plagas De la Cruz López, Norma autora Cruz López, Leopoldo Caridad Doctor autor 2083 Holguín Meléndez, Francisco Doctor autor 2086 Guillén Navarro, Griselda Karina Doctora autora 7945 Huerta Palacios, Graciela Doctora autora 2084 Volatile organic compounds produced by cacao endophytic bacteria and their inhibitory activity on moniliophthora roreri |
description |
Several studies have reported that bacteria produce anti-fungal volatiles. We identifed the organic volatile compounds produced by six cacao endophytic bacteria (CEB) strains (Bacillus amyloliquefaciens CFFSUR-B35, Bacillus megaterium CFFSUR-B32, Bacillus muralis CFFSUR-B39, Bacillus pumilus CFFSUR-B34, Bacillus subtilis CFFSUR-B31, and Novo sphingobium lindaniclasticum CFFSUR-B36). We evaluated their inhibitory efect on mycelium growth and spore germi nation of the phytopathogenic fungus Moniliophtora roreri. The volatiles produced by these six CEB, were collected and identifed by SPME and GC–MS. Moreover, the inhibitory efect of fve synthetic volatile organic compounds, individually and in mixtures (dimethyl disulfde, 2–5 dimethyl pyrazine, α-pinene, 2-heptanone and 2-ethyl hexanol) on M. roreri myce lium growth and spore germination was evaluated. All strains examined produced volatiles in diferent amounts; 13 to 10 compounds were identifed, including sulfde, alcohol benzene derivate, pyrazine, ketone, nitrogen and terpene compounds. The B. subtilis CFFSUR-B31 strain produced the largest number of volatiles, while B. pumilus CFFSUR-B34 produced the fewest and the lowest amounts. The volatile organic compounds produced by B. pumilus CFFSUR-B34, B. muralis CFFSUR B39 and N. lindaniclasticum CFFSUR-B36 inhibited M. roreri mycelium growth by more than 35%, sporulation by more than 81% and spore germination by more than 74%. However, when synthetic compounds were evaluated individually and in mixtures, 2-ethyl hexanol at 100,000 ppm (20 mg/flter paper disc) inhibited M. roreri mycelium growth by 100%, followed by organic volatile compound mixtures C (dimethyl disulfde, 2,5-dimethyl pyrazine, α-pinene, 2-ethyl-hexanol, 2-Heptanone) and D (only the top four) at 100,000 ppm (4 and 5 mg/flter paper disc) which inhibited spore germination by 97 and 89%, respectively. |
format |
Texto |
topic_facet |
Moniliophthora roreri Hongos fitopatógenos Theobroma cacao Compuestos orgánicos volátiles Hongos endófitos Manejo de enfermedades y plagas |
author |
De la Cruz López, Norma autora Cruz López, Leopoldo Caridad Doctor autor 2083 Holguín Meléndez, Francisco Doctor autor 2086 Guillén Navarro, Griselda Karina Doctora autora 7945 Huerta Palacios, Graciela Doctora autora 2084 |
author_facet |
De la Cruz López, Norma autora Cruz López, Leopoldo Caridad Doctor autor 2083 Holguín Meléndez, Francisco Doctor autor 2086 Guillén Navarro, Griselda Karina Doctora autora 7945 Huerta Palacios, Graciela Doctora autora 2084 |
author_sort |
De la Cruz López, Norma autora |
title |
Volatile organic compounds produced by cacao endophytic bacteria and their inhibitory activity on moniliophthora roreri |
title_short |
Volatile organic compounds produced by cacao endophytic bacteria and their inhibitory activity on moniliophthora roreri |
title_full |
Volatile organic compounds produced by cacao endophytic bacteria and their inhibitory activity on moniliophthora roreri |
title_fullStr |
Volatile organic compounds produced by cacao endophytic bacteria and their inhibitory activity on moniliophthora roreri |
title_full_unstemmed |
Volatile organic compounds produced by cacao endophytic bacteria and their inhibitory activity on moniliophthora roreri |
title_sort |
volatile organic compounds produced by cacao endophytic bacteria and their inhibitory activity on moniliophthora roreri |
url |
https://doi.org/10.1007/s00284-021-02696-2 |
work_keys_str_mv |
AT delacruzlopeznormaautora volatileorganiccompoundsproducedbycacaoendophyticbacteriaandtheirinhibitoryactivityonmoniliophthoraroreri AT cruzlopezleopoldocaridaddoctorautor2083 volatileorganiccompoundsproducedbycacaoendophyticbacteriaandtheirinhibitoryactivityonmoniliophthoraroreri AT holguinmelendezfranciscodoctorautor2086 volatileorganiccompoundsproducedbycacaoendophyticbacteriaandtheirinhibitoryactivityonmoniliophthoraroreri AT guillennavarrogriseldakarinadoctoraautora7945 volatileorganiccompoundsproducedbycacaoendophyticbacteriaandtheirinhibitoryactivityonmoniliophthoraroreri AT huertapalaciosgracieladoctoraautora2084 volatileorganiccompoundsproducedbycacaoendophyticbacteriaandtheirinhibitoryactivityonmoniliophthoraroreri |
_version_ |
1806028270588657664 |
spelling |
KOHA-OAI-ECOSUR:620422024-05-15T11:26:32ZVolatile organic compounds produced by cacao endophytic bacteria and their inhibitory activity on moniliophthora roreri De la Cruz López, Norma autora Cruz López, Leopoldo Caridad Doctor autor 2083 Holguín Meléndez, Francisco Doctor autor 2086 Guillén Navarro, Griselda Karina Doctora autora 7945 Huerta Palacios, Graciela Doctora autora 2084 textengSeveral studies have reported that bacteria produce anti-fungal volatiles. We identifed the organic volatile compounds produced by six cacao endophytic bacteria (CEB) strains (Bacillus amyloliquefaciens CFFSUR-B35, Bacillus megaterium CFFSUR-B32, Bacillus muralis CFFSUR-B39, Bacillus pumilus CFFSUR-B34, Bacillus subtilis CFFSUR-B31, and Novo sphingobium lindaniclasticum CFFSUR-B36). We evaluated their inhibitory efect on mycelium growth and spore germi nation of the phytopathogenic fungus Moniliophtora roreri. The volatiles produced by these six CEB, were collected and identifed by SPME and GC–MS. Moreover, the inhibitory efect of fve synthetic volatile organic compounds, individually and in mixtures (dimethyl disulfde, 2–5 dimethyl pyrazine, α-pinene, 2-heptanone and 2-ethyl hexanol) on M. roreri myce lium growth and spore germination was evaluated. All strains examined produced volatiles in diferent amounts; 13 to 10 compounds were identifed, including sulfde, alcohol benzene derivate, pyrazine, ketone, nitrogen and terpene compounds. The B. subtilis CFFSUR-B31 strain produced the largest number of volatiles, while B. pumilus CFFSUR-B34 produced the fewest and the lowest amounts. The volatile organic compounds produced by B. pumilus CFFSUR-B34, B. muralis CFFSUR B39 and N. lindaniclasticum CFFSUR-B36 inhibited M. roreri mycelium growth by more than 35%, sporulation by more than 81% and spore germination by more than 74%. However, when synthetic compounds were evaluated individually and in mixtures, 2-ethyl hexanol at 100,000 ppm (20 mg/flter paper disc) inhibited M. roreri mycelium growth by 100%, followed by organic volatile compound mixtures C (dimethyl disulfde, 2,5-dimethyl pyrazine, α-pinene, 2-ethyl-hexanol, 2-Heptanone) and D (only the top four) at 100,000 ppm (4 and 5 mg/flter paper disc) which inhibited spore germination by 97 and 89%, respectively.Several studies have reported that bacteria produce anti-fungal volatiles. We identifed the organic volatile compounds produced by six cacao endophytic bacteria (CEB) strains (Bacillus amyloliquefaciens CFFSUR-B35, Bacillus megaterium CFFSUR-B32, Bacillus muralis CFFSUR-B39, Bacillus pumilus CFFSUR-B34, Bacillus subtilis CFFSUR-B31, and Novo sphingobium lindaniclasticum CFFSUR-B36). We evaluated their inhibitory efect on mycelium growth and spore germi nation of the phytopathogenic fungus Moniliophtora roreri. The volatiles produced by these six CEB, were collected and identifed by SPME and GC–MS. Moreover, the inhibitory efect of fve synthetic volatile organic compounds, individually and in mixtures (dimethyl disulfde, 2–5 dimethyl pyrazine, α-pinene, 2-heptanone and 2-ethyl hexanol) on M. roreri myce lium growth and spore germination was evaluated. All strains examined produced volatiles in diferent amounts; 13 to 10 compounds were identifed, including sulfde, alcohol benzene derivate, pyrazine, ketone, nitrogen and terpene compounds. The B. subtilis CFFSUR-B31 strain produced the largest number of volatiles, while B. pumilus CFFSUR-B34 produced the fewest and the lowest amounts. The volatile organic compounds produced by B. pumilus CFFSUR-B34, B. muralis CFFSUR B39 and N. lindaniclasticum CFFSUR-B36 inhibited M. roreri mycelium growth by more than 35%, sporulation by more than 81% and spore germination by more than 74%. However, when synthetic compounds were evaluated individually and in mixtures, 2-ethyl hexanol at 100,000 ppm (20 mg/flter paper disc) inhibited M. roreri mycelium growth by 100%, followed by organic volatile compound mixtures C (dimethyl disulfde, 2,5-dimethyl pyrazine, α-pinene, 2-ethyl-hexanol, 2-Heptanone) and D (only the top four) at 100,000 ppm (4 and 5 mg/flter paper disc) which inhibited spore germination by 97 and 89%, respectively.Moniliophthora roreriHongos fitopatógenosTheobroma cacaoCompuestos orgánicos volátilesHongos endófitosManejo de enfermedades y plagasCurrent Microbiologyhttps://doi.org/10.1007/s00284-021-02696-2Disponible para usuarios de ECOSUR con su clave de acceso |