Prevalence of antibodies against Entamoeba histolytica in Mexico measured by ELISA
The prevalence of antibodies against Entamoeba histolytica was studied in the Mexican population using an immunoenzyme assay in solid phase (ELISA) and semiautomatic equipment. The antigen was a mixture of membrane proteins obtained by Triton X-100 extraction from an axenic culture of Entamoeba histolytica HM1-IMSS. The method was standardized by comparing serum samples from amoebic liver abscess patients with healthy volunteers. From the 60538 samples supplied by the National Seroepidemiology Survey, antibodies were found in 4·49% (4·32-4·65% at 95% confidence limit). More significant titres occurred in the central region of the country. The ratio female to male was 1·25:1. The population living in metropolitan areas had probably been infected at a younger age than those living in the country. Important differences were found in the seroprevalence obtained by ELISA compared with a study which used indirect haemagglutination (IHA) in the same sample frame
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Texto biblioteca |
Language: | eng |
Subjects: | Entamoeba histolytica, Amoebiasis, Epidemiología, Encuestas serológicas, |
Online Access: | https://doi.org/10.1017/S0950268800058702 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
KOHA-OAI-ECOSUR:54401 |
---|---|
record_format |
koha |
spelling |
KOHA-OAI-ECOSUR:544012024-03-12T12:53:37ZPrevalence of antibodies against Entamoeba histolytica in Mexico measured by ELISA González, C. R. Isibasi, A. autor/a Ortiz Navarrete, V. autor/a Paniagua Mijangos, Jorge Gustavo autor García, J. A autor/a Ramírez, A. autor/a Salvatierra Izaba, Ernesto Benito Doctor autor 8260 Tapia, R. autor/a Sepúlveda, Jaime autor Gutiérrez, G. K. autor/a Kumate, Jesús autor textengThe prevalence of antibodies against Entamoeba histolytica was studied in the Mexican population using an immunoenzyme assay in solid phase (ELISA) and semiautomatic equipment. The antigen was a mixture of membrane proteins obtained by Triton X-100 extraction from an axenic culture of Entamoeba histolytica HM1-IMSS. The method was standardized by comparing serum samples from amoebic liver abscess patients with healthy volunteers. From the 60538 samples supplied by the National Seroepidemiology Survey, antibodies were found in 4·49% (4·32-4·65% at 95% confidence limit). More significant titres occurred in the central region of the country. The ratio female to male was 1·25:1. The population living in metropolitan areas had probably been infected at a younger age than those living in the country. Important differences were found in the seroprevalence obtained by ELISA compared with a study which used indirect haemagglutination (IHA) in the same sample frameThe prevalence of antibodies against Entamoeba histolytica was studied in the Mexican population using an immunoenzyme assay in solid phase (ELISA) and semiautomatic equipment. The antigen was a mixture of membrane proteins obtained by Triton X-100 extraction from an axenic culture of Entamoeba histolytica HM1-IMSS. The method was standardized by comparing serum samples from amoebic liver abscess patients with healthy volunteers. From the 60538 samples supplied by the National Seroepidemiology Survey, antibodies were found in 4·49% (4·32-4·65% at 95% confidence limit). More significant titres occurred in the central region of the country. The ratio female to male was 1·25:1. The population living in metropolitan areas had probably been infected at a younger age than those living in the country. Important differences were found in the seroprevalence obtained by ELISA compared with a study which used indirect haemagglutination (IHA) in the same sample frameEntamoeba histolyticaAmoebiasisEpidemiologíaEncuestas serológicasEpidemiol Infecthttps://doi.org/10.1017/S0950268800058702Disponible para usuarios de ECOSUR con su clave de acceso |
institution |
ECOSUR |
collection |
Koha |
country |
México |
countrycode |
MX |
component |
Bibliográfico |
access |
En linea En linea |
databasecode |
cat-ecosur |
tag |
biblioteca |
region |
America del Norte |
libraryname |
Sistema de Información Bibliotecario de ECOSUR (SIBE) |
language |
eng |
topic |
Entamoeba histolytica Amoebiasis Epidemiología Encuestas serológicas Entamoeba histolytica Amoebiasis Epidemiología Encuestas serológicas |
spellingShingle |
Entamoeba histolytica Amoebiasis Epidemiología Encuestas serológicas Entamoeba histolytica Amoebiasis Epidemiología Encuestas serológicas González, C. R. Isibasi, A. autor/a Ortiz Navarrete, V. autor/a Paniagua Mijangos, Jorge Gustavo autor García, J. A autor/a Ramírez, A. autor/a Salvatierra Izaba, Ernesto Benito Doctor autor 8260 Tapia, R. autor/a Sepúlveda, Jaime autor Gutiérrez, G. K. autor/a Kumate, Jesús autor Prevalence of antibodies against Entamoeba histolytica in Mexico measured by ELISA |
description |
The prevalence of antibodies against Entamoeba histolytica was studied in the Mexican population using an immunoenzyme assay in solid phase (ELISA) and semiautomatic equipment. The antigen was a mixture of membrane proteins obtained by Triton X-100 extraction from an axenic culture of Entamoeba histolytica HM1-IMSS. The method was standardized by comparing serum samples from amoebic liver abscess patients with healthy volunteers. From the 60538 samples supplied by the National Seroepidemiology Survey, antibodies were found in 4·49% (4·32-4·65% at 95% confidence limit). More significant titres occurred in the central region of the country. The ratio female to male was 1·25:1. The population living in metropolitan areas had probably been infected at a younger age than those living in the country. Important differences were found in the seroprevalence obtained by ELISA compared with a study which used indirect haemagglutination (IHA) in the same sample frame |
format |
Texto |
topic_facet |
Entamoeba histolytica Amoebiasis Epidemiología Encuestas serológicas |
author |
González, C. R. Isibasi, A. autor/a Ortiz Navarrete, V. autor/a Paniagua Mijangos, Jorge Gustavo autor García, J. A autor/a Ramírez, A. autor/a Salvatierra Izaba, Ernesto Benito Doctor autor 8260 Tapia, R. autor/a Sepúlveda, Jaime autor Gutiérrez, G. K. autor/a Kumate, Jesús autor |
author_facet |
González, C. R. Isibasi, A. autor/a Ortiz Navarrete, V. autor/a Paniagua Mijangos, Jorge Gustavo autor García, J. A autor/a Ramírez, A. autor/a Salvatierra Izaba, Ernesto Benito Doctor autor 8260 Tapia, R. autor/a Sepúlveda, Jaime autor Gutiérrez, G. K. autor/a Kumate, Jesús autor |
author_sort |
González, C. R. |
title |
Prevalence of antibodies against Entamoeba histolytica in Mexico measured by ELISA |
title_short |
Prevalence of antibodies against Entamoeba histolytica in Mexico measured by ELISA |
title_full |
Prevalence of antibodies against Entamoeba histolytica in Mexico measured by ELISA |
title_fullStr |
Prevalence of antibodies against Entamoeba histolytica in Mexico measured by ELISA |
title_full_unstemmed |
Prevalence of antibodies against Entamoeba histolytica in Mexico measured by ELISA |
title_sort |
prevalence of antibodies against entamoeba histolytica in mexico measured by elisa |
url |
https://doi.org/10.1017/S0950268800058702 |
work_keys_str_mv |
AT gonzalezcr prevalenceofantibodiesagainstentamoebahistolyticainmexicomeasuredbyelisa AT isibasiaautora prevalenceofantibodiesagainstentamoebahistolyticainmexicomeasuredbyelisa AT ortiznavarretevautora prevalenceofantibodiesagainstentamoebahistolyticainmexicomeasuredbyelisa AT paniaguamijangosjorgegustavoautor prevalenceofantibodiesagainstentamoebahistolyticainmexicomeasuredbyelisa AT garciajaautora prevalenceofantibodiesagainstentamoebahistolyticainmexicomeasuredbyelisa AT ramirezaautora prevalenceofantibodiesagainstentamoebahistolyticainmexicomeasuredbyelisa AT salvatierraizabaernestobenitodoctorautor8260 prevalenceofantibodiesagainstentamoebahistolyticainmexicomeasuredbyelisa AT tapiarautora prevalenceofantibodiesagainstentamoebahistolyticainmexicomeasuredbyelisa AT sepulvedajaimeautor prevalenceofantibodiesagainstentamoebahistolyticainmexicomeasuredbyelisa AT gutierrezgkautora prevalenceofantibodiesagainstentamoebahistolyticainmexicomeasuredbyelisa AT kumatejesusautor prevalenceofantibodiesagainstentamoebahistolyticainmexicomeasuredbyelisa |
_version_ |
1794791709399318528 |