In vitro evaluation of methanol extracts of edible fungi Pleurotus ostreatus and Lentinula edodes against Rhyssomatus nigerrimus Fahraeus
The study aimed to evaluate the insecticidal activity of extracts of edible mushrooms Pleurotus ostreatus and Lentinula edodes against Rhyssomatus nigerrimus. Methanol:water extracts (70:30) of P. ostreatus and L. edodes were made and evaluated in two in vitro tests (exposure and immersion toxic effect) against R. nigerrimus. Subsequently, the toxicity test of the extracts against Artemia salina was evaluated. These extracts were subjected to colorimetric tests and gas chromatography-mass spectrometry. The results showed a mortality effect against R. nigerrimus of 50% for the P. ostreatus 2 extracts at a concentration of 20% in the immersion test. Likewise, in the toxic effect test, 90% mortality was observed after five days of exposure to a concentration of 10%. On the other hand, for the toxicity test, the extract that showed the values with the highest mortality against A. salina was P. ostreatus, starting with 80% mortality at 100μg/mL. The functional groups present in the extracts were saponins, coumarins, and alkaloids. Furthermore, the presence of more than 7 compounds in the mushroom extracts evaluated is reported. This study demonstrates the insecticidal activity of P. ostreatus and L. edodes fungal extracts and indicates the importance of using different in vitro tests to elucidate the mechanism of action for future studies.
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| Subjects: | Hongos comestibles, Pleurotus ostreatus, Lentinula edodes, Rhyssomatus nigerrimus, Insecticidas biológicos, Soya, Control de plagas, Artfrosur, |
| Online Access: | https://doi.org/10.1590/1519-6984.276127 |
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Hongos comestibles Pleurotus ostreatus Lentinula edodes Rhyssomatus nigerrimus Insecticidas biológicos Soya Control de plagas Artfrosur Hongos comestibles Pleurotus ostreatus Lentinula edodes Rhyssomatus nigerrimus Insecticidas biológicos Soya Control de plagas Artfrosur |
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Hongos comestibles Pleurotus ostreatus Lentinula edodes Rhyssomatus nigerrimus Insecticidas biológicos Soya Control de plagas Artfrosur Hongos comestibles Pleurotus ostreatus Lentinula edodes Rhyssomatus nigerrimus Insecticidas biológicos Soya Control de plagas Artfrosur Castañeda Ramírez, Gloria Sarahí Doctora autora 22568 López Guillén, Guillermo autor 13994 Cruz López, Leopoldo Caridad Doctor autor 2083 Hernández Núñez, E. autor/a Wong Villarreal, A. autor/a Sánchez, José E. Doctor autor 5443 Aguilar Marcelino, Liliana autora 14446 In vitro evaluation of methanol extracts of edible fungi Pleurotus ostreatus and Lentinula edodes against Rhyssomatus nigerrimus Fahraeus |
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The study aimed to evaluate the insecticidal activity of extracts of edible mushrooms Pleurotus ostreatus and Lentinula edodes against Rhyssomatus nigerrimus. Methanol:water extracts (70:30) of P. ostreatus and L. edodes were made and evaluated in two in vitro tests (exposure and immersion toxic effect) against R. nigerrimus. Subsequently, the toxicity test of the extracts against Artemia salina was evaluated. These extracts were subjected to colorimetric tests and gas chromatography-mass spectrometry. The results showed a mortality effect against R. nigerrimus of 50% for the P. ostreatus 2 extracts at a concentration of 20% in the immersion test. Likewise, in the toxic effect test, 90% mortality was observed after five days of exposure to a concentration of 10%. On the other hand, for the toxicity test, the extract that showed the values with the highest mortality against A. salina was P. ostreatus, starting with 80% mortality at 100μg/mL. The functional groups present in the extracts were saponins, coumarins, and alkaloids. Furthermore, the presence of more than 7 compounds in the mushroom extracts evaluated is reported. This study demonstrates the insecticidal activity of P. ostreatus and L. edodes fungal extracts and indicates the importance of using different in vitro tests to elucidate the mechanism of action for future studies. |
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Hongos comestibles Pleurotus ostreatus Lentinula edodes Rhyssomatus nigerrimus Insecticidas biológicos Soya Control de plagas Artfrosur |
| author |
Castañeda Ramírez, Gloria Sarahí Doctora autora 22568 López Guillén, Guillermo autor 13994 Cruz López, Leopoldo Caridad Doctor autor 2083 Hernández Núñez, E. autor/a Wong Villarreal, A. autor/a Sánchez, José E. Doctor autor 5443 Aguilar Marcelino, Liliana autora 14446 |
| author_facet |
Castañeda Ramírez, Gloria Sarahí Doctora autora 22568 López Guillén, Guillermo autor 13994 Cruz López, Leopoldo Caridad Doctor autor 2083 Hernández Núñez, E. autor/a Wong Villarreal, A. autor/a Sánchez, José E. Doctor autor 5443 Aguilar Marcelino, Liliana autora 14446 |
| author_sort |
Castañeda Ramírez, Gloria Sarahí Doctora autora 22568 |
| title |
In vitro evaluation of methanol extracts of edible fungi Pleurotus ostreatus and Lentinula edodes against Rhyssomatus nigerrimus Fahraeus |
| title_short |
In vitro evaluation of methanol extracts of edible fungi Pleurotus ostreatus and Lentinula edodes against Rhyssomatus nigerrimus Fahraeus |
| title_full |
In vitro evaluation of methanol extracts of edible fungi Pleurotus ostreatus and Lentinula edodes against Rhyssomatus nigerrimus Fahraeus |
| title_fullStr |
In vitro evaluation of methanol extracts of edible fungi Pleurotus ostreatus and Lentinula edodes against Rhyssomatus nigerrimus Fahraeus |
| title_full_unstemmed |
In vitro evaluation of methanol extracts of edible fungi Pleurotus ostreatus and Lentinula edodes against Rhyssomatus nigerrimus Fahraeus |
| title_sort |
in vitro evaluation of methanol extracts of edible fungi pleurotus ostreatus and lentinula edodes against rhyssomatus nigerrimus fahraeus |
| url |
https://doi.org/10.1590/1519-6984.276127 |
| work_keys_str_mv |
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KOHA-OAI-ECOSUR:542682024-06-12T21:26:32ZIn vitro evaluation of methanol extracts of edible fungi Pleurotus ostreatus and Lentinula edodes against Rhyssomatus nigerrimus Fahraeus Castañeda Ramírez, Gloria Sarahí Doctora autora 22568 López Guillén, Guillermo autor 13994 Cruz López, Leopoldo Caridad Doctor autor 2083 Hernández Núñez, E. autor/a Wong Villarreal, A. autor/a Sánchez, José E. Doctor autor 5443 Aguilar Marcelino, Liliana autora 14446 textengThe study aimed to evaluate the insecticidal activity of extracts of edible mushrooms Pleurotus ostreatus and Lentinula edodes against Rhyssomatus nigerrimus. Methanol:water extracts (70:30) of P. ostreatus and L. edodes were made and evaluated in two in vitro tests (exposure and immersion toxic effect) against R. nigerrimus. Subsequently, the toxicity test of the extracts against Artemia salina was evaluated. These extracts were subjected to colorimetric tests and gas chromatography-mass spectrometry. The results showed a mortality effect against R. nigerrimus of 50% for the P. ostreatus 2 extracts at a concentration of 20% in the immersion test. Likewise, in the toxic effect test, 90% mortality was observed after five days of exposure to a concentration of 10%. On the other hand, for the toxicity test, the extract that showed the values with the highest mortality against A. salina was P. ostreatus, starting with 80% mortality at 100μg/mL. The functional groups present in the extracts were saponins, coumarins, and alkaloids. Furthermore, the presence of more than 7 compounds in the mushroom extracts evaluated is reported. This study demonstrates the insecticidal activity of P. ostreatus and L. edodes fungal extracts and indicates the importance of using different in vitro tests to elucidate the mechanism of action for future studies.O estudo teve como objetivo avaliar a atividade inseticida dos extratos dos cogumelos comestíveis Pleurotus ostreatus e Lentinula edodes contra Rhyssomatus nigerrimus. Extratos metanol:água (70:30) de P. ostreatus e L. edodes foram elaborados e avaliados em dois testes in vitro (efeito tóxico de exposição e imersão) contra R. nigerrimus. Posteriormente foi avaliado o teste de toxicidade dos extratos frente à Artemia salina. Esses extratos foram submetidos a testes colorimétricos e cromatografia gasosa-espectrometria de massas. Os resultados mostraram efeito de mortalidade contra R. nigerrimus de 50% para o extrato de P. ostreatus 2 na concentração de 20% no teste de imersão. Da mesma forma, no teste de efeito tóxico, foi observada mortalidade de 90% após 5 dias de exposição à concentração de 10%. Por outro lado, para o teste de toxicidade, o extrato que apresentou os valores com maior mortalidade contra A. salina foi P. ostreatus, iniciando com 80% de mortalidade a 100μg/mL. Os grupos funcionais presentes nos extratos foram saponinas, cumarinas e alcaloides. Além disso, é relatada a presença de mais de 7 compostos nos extratos de cogumelos avaliados. Este estudo demonstra a atividade inseticida dos extratos fúngicos de P. ostreatus e L. edodes e indica a importância da utilização de diferentes testes in vitro para elucidar o mecanismo de ação para estudos futuros.The study aimed to evaluate the insecticidal activity of extracts of edible mushrooms Pleurotus ostreatus and Lentinula edodes against Rhyssomatus nigerrimus. Methanol:water extracts (70:30) of P. ostreatus and L. edodes were made and evaluated in two in vitro tests (exposure and immersion toxic effect) against R. nigerrimus. Subsequently, the toxicity test of the extracts against Artemia salina was evaluated. These extracts were subjected to colorimetric tests and gas chromatography-mass spectrometry. The results showed a mortality effect against R. nigerrimus of 50% for the P. ostreatus 2 extracts at a concentration of 20% in the immersion test. Likewise, in the toxic effect test, 90% mortality was observed after five days of exposure to a concentration of 10%. On the other hand, for the toxicity test, the extract that showed the values with the highest mortality against A. salina was P. ostreatus, starting with 80% mortality at 100μg/mL. The functional groups present in the extracts were saponins, coumarins, and alkaloids. Furthermore, the presence of more than 7 compounds in the mushroom extracts evaluated is reported. This study demonstrates the insecticidal activity of P. ostreatus and L. edodes fungal extracts and indicates the importance of using different in vitro tests to elucidate the mechanism of action for future studies.O estudo teve como objetivo avaliar a atividade inseticida dos extratos dos cogumelos comestíveis Pleurotus ostreatus e Lentinula edodes contra Rhyssomatus nigerrimus. Extratos metanol:água (70:30) de P. ostreatus e L. edodes foram elaborados e avaliados em dois testes in vitro (efeito tóxico de exposição e imersão) contra R. nigerrimus. Posteriormente foi avaliado o teste de toxicidade dos extratos frente à Artemia salina. Esses extratos foram submetidos a testes colorimétricos e cromatografia gasosa-espectrometria de massas. Os resultados mostraram efeito de mortalidade contra R. nigerrimus de 50% para o extrato de P. ostreatus 2 na concentração de 20% no teste de imersão. Da mesma forma, no teste de efeito tóxico, foi observada mortalidade de 90% após 5 dias de exposição à concentração de 10%. Por outro lado, para o teste de toxicidade, o extrato que apresentou os valores com maior mortalidade contra A. salina foi P. ostreatus, iniciando com 80% de mortalidade a 100μg/mL. Os grupos funcionais presentes nos extratos foram saponinas, cumarinas e alcaloides. Além disso, é relatada a presença de mais de 7 compostos nos extratos de cogumelos avaliados. Este estudo demonstra a atividade inseticida dos extratos fúngicos de P. ostreatus e L. edodes e indica a importância da utilização de diferentes testes in vitro para elucidar o mecanismo de ação para estudos futuros.Hongos comestiblesPleurotus ostreatusLentinula edodesRhyssomatus nigerrimusInsecticidas biológicosSoyaControl de plagasArtfrosurBrazilian Journal of Biologyhttps://doi.org/10.1590/1519-6984.276127Acceso en línea sin restricciones |