DIG-ELISA test in onchocerciasis. Serum antibodies and probability of infection
An immunoenzymatic test (DIG-ELISA) was serologically evaluated for the serodiagnosis of onchocerciasis. Control and infected sera from the onchocerciasis endemic area of Mexico was collected and the donors assessed for onchocerciasis according to parasitological, clinical, and epidemiological data. The sera were submitted to the DIG-ELISA test using a crude extract prepared from O. volvulus adult worms which had been preservar in nodules in 67% glycerol. The test showed a 100% sensitivity with sera from 38 microfilariae carriers and 96% specificity with sera from 133 non-infected people living outside the endemic zone. In addition, seropositivity was 52.9 % with samples from non- onchocercotic people living inside the endemic area, while 82.5 and 90.5% of sera from patients with clinical symptoms and subcutaneous nodules, respectively, were positive.
| Main Authors: | , , , |
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| Format: | Texto biblioteca |
| Language: | eng |
| Subjects: | Oncocercosis, Diagnóstico, Onchocerca volvulus, Artfrosur, |
| Online Access: | http://bvs.insp.mx/articulos/5/23/111998.htm |
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