DIG-ELISA test in onchocerciasis. Serum antibodies and probability of infection

DIG-ELISA test in onchocerciasis. Serum antibodies and probability of infection

An immunoenzymatic test (DIG-ELISA) was serologically evaluated for the serodiagnosis of onchocerciasis. Control and infected sera from the onchocerciasis endemic area of Mexico was collected and the donors assessed for onchocerciasis according to parasitological, clinical, and epidemiological data. The sera were submitted to the DIG-ELISA test using a crude extract prepared from O. volvulus adult worms which had been preservar in nodules in 67% glycerol. The test showed a 100% sensitivity with sera from 38 microfilariae carriers and 96% specificity with sera from 133 non-infected people living outside the endemic zone. In addition, seropositivity was 52.9 % with samples from non- onchocercotic people living inside the endemic area, while 82.5 and 90.5% of sera from patients with clinical symptoms and subcutaneous nodules, respectively, were positive.

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Bibliographic Details
Main Authors: Gómez Priego, Alberto Maestro 20960, Cruz Gutiérrez, Luis Enrique autor/a, Paniagua Solís, Jorge Fernando autor/a, Ruenes Meza, María Teresa autor/a
Format: Texto biblioteca
Language:eng
Subjects:Oncocercosis, Diagnóstico, Onchocerca volvulus, Artfrosur,
Online Access:http://bvs.insp.mx/articulos/5/23/111998.htm
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spelling KOHA-OAI-ECOSUR:478242023-07-18T11:26:46ZDIG-ELISA test in onchocerciasis. Serum antibodies and probability of infection Gómez Priego, Alberto Maestro 20960 Cruz Gutiérrez, Luis Enrique autor/a Paniagua Solís, Jorge Fernando autor/a Ruenes Meza, María Teresa autor/a textengAn immunoenzymatic test (DIG-ELISA) was serologically evaluated for the serodiagnosis of onchocerciasis. Control and infected sera from the onchocerciasis endemic area of Mexico was collected and the donors assessed for onchocerciasis according to parasitological, clinical, and epidemiological data. The sera were submitted to the DIG-ELISA test using a crude extract prepared from O. volvulus adult worms which had been preservar in nodules in 67% glycerol. The test showed a 100% sensitivity with sera from 38 microfilariae carriers and 96% specificity with sera from 133 non-infected people living outside the endemic zone. In addition, seropositivity was 52.9 % with samples from non- onchocercotic people living inside the endemic area, while 82.5 and 90.5% of sera from patients with clinical symptoms and subcutaneous nodules, respectively, were positive.A high rate (30%) of cross-reactivity with serum samples from people infected with Wuchereria bancroftri or Brugia timori was obtained, which is in contrast with the low seropositivity rates (7.4%) obtained with sera from patients infected with other parasites. These results suggest that DIG-ELISA test may be a useful serological test for antibody detection in onchocerciasis, especially for epidemiological surveillance of disease, but because of the high cross-reactivity observed, its use must be limited to areas where this parasitic infection does not coexist with other human filiariasis. Finally, the arrangement of sera in groups according to the relative likelihood to have onchocerciasis seems to be a useful procedure for evaluation of serological tests. (Arch Med Res 1993; 24:353).An immunoenzymatic test (DIG-ELISA) was serologically evaluated for the serodiagnosis of onchocerciasis. Control and infected sera from the onchocerciasis endemic area of Mexico was collected and the donors assessed for onchocerciasis according to parasitological, clinical, and epidemiological data. The sera were submitted to the DIG-ELISA test using a crude extract prepared from O. volvulus adult worms which had been preservar in nodules in 67% glycerol. The test showed a 100% sensitivity with sera from 38 microfilariae carriers and 96% specificity with sera from 133 non-infected people living outside the endemic zone. In addition, seropositivity was 52.9 % with samples from non- onchocercotic people living inside the endemic area, while 82.5 and 90.5% of sera from patients with clinical symptoms and subcutaneous nodules, respectively, were positive.A high rate (30%) of cross-reactivity with serum samples from people infected with Wuchereria bancroftri or Brugia timori was obtained, which is in contrast with the low seropositivity rates (7.4%) obtained with sera from patients infected with other parasites. These results suggest that DIG-ELISA test may be a useful serological test for antibody detection in onchocerciasis, especially for epidemiological surveillance of disease, but because of the high cross-reactivity observed, its use must be limited to areas where this parasitic infection does not coexist with other human filiariasis. Finally, the arrangement of sera in groups according to the relative likelihood to have onchocerciasis seems to be a useful procedure for evaluation of serological tests. (Arch Med Res 1993; 24:353).Requerimiento del sistema: Adobe Acrobat profesional 6.0 o superior e InternetOncocercosisDiagnósticoOnchocerca volvulusArtfrosurDisponible en líneaArchives of Medical Researchhttp://bvs.insp.mx/articulos/5/23/111998.htmAcceso en línea sin restricciones
institution ECOSUR
collection Koha
country México
countrycode MX
component Bibliográfico
access En linea
En linea
databasecode cat-ecosur
tag biblioteca
region America del Norte
libraryname Sistema de Información Bibliotecario de ECOSUR (SIBE)
language eng
topic Oncocercosis
Diagnóstico
Onchocerca volvulus
Artfrosur
Oncocercosis
Diagnóstico
Onchocerca volvulus
Artfrosur
spellingShingle Oncocercosis
Diagnóstico
Onchocerca volvulus
Artfrosur
Oncocercosis
Diagnóstico
Onchocerca volvulus
Artfrosur
Gómez Priego, Alberto Maestro 20960
Cruz Gutiérrez, Luis Enrique autor/a
Paniagua Solís, Jorge Fernando autor/a
Ruenes Meza, María Teresa autor/a
DIG-ELISA test in onchocerciasis. Serum antibodies and probability of infection
description An immunoenzymatic test (DIG-ELISA) was serologically evaluated for the serodiagnosis of onchocerciasis. Control and infected sera from the onchocerciasis endemic area of Mexico was collected and the donors assessed for onchocerciasis according to parasitological, clinical, and epidemiological data. The sera were submitted to the DIG-ELISA test using a crude extract prepared from O. volvulus adult worms which had been preservar in nodules in 67% glycerol. The test showed a 100% sensitivity with sera from 38 microfilariae carriers and 96% specificity with sera from 133 non-infected people living outside the endemic zone. In addition, seropositivity was 52.9 % with samples from non- onchocercotic people living inside the endemic area, while 82.5 and 90.5% of sera from patients with clinical symptoms and subcutaneous nodules, respectively, were positive.
format Texto
topic_facet Oncocercosis
Diagnóstico
Onchocerca volvulus
Artfrosur
author Gómez Priego, Alberto Maestro 20960
Cruz Gutiérrez, Luis Enrique autor/a
Paniagua Solís, Jorge Fernando autor/a
Ruenes Meza, María Teresa autor/a
author_facet Gómez Priego, Alberto Maestro 20960
Cruz Gutiérrez, Luis Enrique autor/a
Paniagua Solís, Jorge Fernando autor/a
Ruenes Meza, María Teresa autor/a
author_sort Gómez Priego, Alberto Maestro 20960
title DIG-ELISA test in onchocerciasis. Serum antibodies and probability of infection
title_short DIG-ELISA test in onchocerciasis. Serum antibodies and probability of infection
title_full DIG-ELISA test in onchocerciasis. Serum antibodies and probability of infection
title_fullStr DIG-ELISA test in onchocerciasis. Serum antibodies and probability of infection
title_full_unstemmed DIG-ELISA test in onchocerciasis. Serum antibodies and probability of infection
title_sort dig-elisa test in onchocerciasis. serum antibodies and probability of infection
url http://bvs.insp.mx/articulos/5/23/111998.htm
work_keys_str_mv AT gomezpriegoalbertomaestro20960 digelisatestinonchocerciasisserumantibodiesandprobabilityofinfection
AT cruzgutierrezluisenriqueautora digelisatestinonchocerciasisserumantibodiesandprobabilityofinfection
AT paniaguasolisjorgefernandoautora digelisatestinonchocerciasisserumantibodiesandprobabilityofinfection
AT ruenesmezamariateresaautora digelisatestinonchocerciasisserumantibodiesandprobabilityofinfection
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