Somatic embryogenesis from three coffee cultivars: 'Rubi', 'Catuai Vermelho 81', and 'IAPAR 59'

Leaf explants of Coffea arabica cultivars Rubi, Catua{DD} Vermelho 81 and IAPAR 59 were in vitro cultured on MS medium with 4 micro M of picloram for 8-12 weeks in the dark at 27 plus or minus 2 deg C (standard temperature). Rubi and Catuaí Vermelho 81 explants with callus were transferred to an SP basal medium plus 0, 12, 24, and 48 micro M benzyladenine, whereas for IAPAR 59, only 48 micro M benzyladenine was added. After 8 weeks for Rubi and Catua{DD} Vermelho 81 and after 12 weeks for IAPAR 59, all cultivars showed indirect embryogenesis. Embryogenic material was transferred to an SP basal medium without benzyladenine, in light (16-hour photoperiod) and standard temperature. In a second experiment, leaf explants belonging to all 3 cultivars were inoculated in petri dishes with SP basal medium plus 0, 2.5 and 5 micro M benzyladenine, in the dark at standard temperature. After 8 weeks, only IAPAR 59 produced direct embryos. These were transferred to SP basal medium. Approximately 30 percent ofthe embryos developed abnormalities. Normal embryos were successfully transferred to soil.

Bibliographic Details

Main Authors:	57684 Cid, L.P.B., 60136 Cruz, A.R.R., 55572 Castro, L.H.R.
Format:	biblioteca
Published:	2004
Subjects:	COFFEA, COFFEA ARABICA, RUBIACEAE, RUBIALES, DICOTILEDONEAS, ANGIOSPERMAS, SPERMATOPHYTA, TRASTORNOS FUNCIONALES, 6-BENZILADENINA, MEDIO DE CULTIVO, CULTIVO IN VITRO, REGENERACION IN VITRO, HOJAS, PICLORAN, SUSTANCIAS DE CRECIMIENTO VEGETAL, EMBRIOGENESIS SOMATICA, EMBRION SOMATICO, CULTIVO DE TEJIDOS,
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