Effect of BAP concentrations in pre-culture medium and BAP and TDZ in sub-culture medium on coffee micropropagation
The residual effects of BAP [benzyladenine] in a pre-culture medium and BAP and TDZ (thidiazuron) in a sub-culture medium on coffee axillary bud proliferation were evaluated. Nodal segments of coffee cv. Caturra Amarelo established in vitro were pre-cultured in MS medium without the growth regulators or with BAP at 6 mg/litre. After 180 days, the nodal segments were inoculated in MS medium supplemented with BAP at 0, 6, 9, 12 and 15 mg/litre, in the first experiment. In the second experiment, the nodal segments were inoculated in MS medium containing TDZ at 0, 0.05, 0.15, 0.45 and 1.35 mg/litre. The pH of the medium was adjusted to 5.8 and solidified with 0.7
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2002
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| Subjects: | COFFEA, COFFEA ARABICA, 6-BENZILADENINA, YEMA (PLANTA), CAFE, MEDIO DE CULTIVO, CULTIVO IN VITRO, MICROPROPAGACION, SUSTANCIAS DE CRECIMIENTO VEGETAL, EFECTOS RESIDUALES, BROTACION, TIDIAZURON, CULTIVO DE TEJIDOS, |
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KOHA-OAI-BVE:891662020-02-03T21:57:01ZEffect of BAP concentrations in pre-culture medium and BAP and TDZ in sub-culture medium on coffee micropropagationMicropropagacao do cafeeiro com concentracoes de BAP em meio de pre-cultivo e de BAP e TDZ em meio de subcultivo 80886 Jesús, A.M.S. 54822 Carvalho, S.P. de 103528 Pasqual, M. 54799 Carvalho, M. 63857 Dutra, L.F. 2002The residual effects of BAP [benzyladenine] in a pre-culture medium and BAP and TDZ (thidiazuron) in a sub-culture medium on coffee axillary bud proliferation were evaluated. Nodal segments of coffee cv. Caturra Amarelo established in vitro were pre-cultured in MS medium without the growth regulators or with BAP at 6 mg/litre. After 180 days, the nodal segments were inoculated in MS medium supplemented with BAP at 0, 6, 9, 12 and 15 mg/litre, in the first experiment. In the second experiment, the nodal segments were inoculated in MS medium containing TDZ at 0, 0.05, 0.15, 0.45 and 1.35 mg/litre. The pH of the medium was adjusted to 5.8 and solidified with 0.7agar. Explants were then transferred to the growth room at 27.1°C temperature, 16 h photoperiod and 32 AM m-2 s-1 light intensity for 120 days. There was a residual effect of BAP added to the pre-culture medium. Addition of BAP (9-10 mg/litre) and TDZ (0.15-1.35 mg/litre) to the sub-culture medium increased the total number of sprouts, number of sprouts between 0.5 and 1.0 cm, number of sprouts larger than 1.0 cm, and sprout fresh matter weight.The residual effects of BAP [benzyladenine] in a pre-culture medium and BAP and TDZ (thidiazuron) in a sub-culture medium on coffee axillary bud proliferation were evaluated. Nodal segments of coffee cv. Caturra Amarelo established in vitro were pre-cultured in MS medium without the growth regulators or with BAP at 6 mg/litre. After 180 days, the nodal segments were inoculated in MS medium supplemented with BAP at 0, 6, 9, 12 and 15 mg/litre, in the first experiment. In the second experiment, the nodal segments were inoculated in MS medium containing TDZ at 0, 0.05, 0.15, 0.45 and 1.35 mg/litre. The pH of the medium was adjusted to 5.8 and solidified with 0.7agar. Explants were then transferred to the growth room at 27.1°C temperature, 16 h photoperiod and 32 AM m-2 s-1 light intensity for 120 days. There was a residual effect of BAP added to the pre-culture medium. Addition of BAP (9-10 mg/litre) and TDZ (0.15-1.35 mg/litre) to the sub-culture medium increased the total number of sprouts, number of sprouts between 0.5 and 1.0 cm, number of sprouts larger than 1.0 cm, and sprout fresh matter weight.COFFEACOFFEA ARABICA6-BENZILADENINAYEMA (PLANTA)CAFEMEDIO DE CULTIVOCULTIVO IN VITROMICROPROPAGACIONSUSTANCIAS DE CRECIMIENTO VEGETALEFECTOS RESIDUALESBROTACIONTIDIAZURONCULTIVO DE TEJIDOSRevista Ceres (Brasil) |
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COFFEA COFFEA ARABICA 6-BENZILADENINA YEMA (PLANTA) CAFE MEDIO DE CULTIVO CULTIVO IN VITRO MICROPROPAGACION SUSTANCIAS DE CRECIMIENTO VEGETAL EFECTOS RESIDUALES BROTACION TIDIAZURON CULTIVO DE TEJIDOS COFFEA COFFEA ARABICA 6-BENZILADENINA YEMA (PLANTA) CAFE MEDIO DE CULTIVO CULTIVO IN VITRO MICROPROPAGACION SUSTANCIAS DE CRECIMIENTO VEGETAL EFECTOS RESIDUALES BROTACION TIDIAZURON CULTIVO DE TEJIDOS |
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COFFEA COFFEA ARABICA 6-BENZILADENINA YEMA (PLANTA) CAFE MEDIO DE CULTIVO CULTIVO IN VITRO MICROPROPAGACION SUSTANCIAS DE CRECIMIENTO VEGETAL EFECTOS RESIDUALES BROTACION TIDIAZURON CULTIVO DE TEJIDOS COFFEA COFFEA ARABICA 6-BENZILADENINA YEMA (PLANTA) CAFE MEDIO DE CULTIVO CULTIVO IN VITRO MICROPROPAGACION SUSTANCIAS DE CRECIMIENTO VEGETAL EFECTOS RESIDUALES BROTACION TIDIAZURON CULTIVO DE TEJIDOS 80886 Jesús, A.M.S. 54822 Carvalho, S.P. de 103528 Pasqual, M. 54799 Carvalho, M. 63857 Dutra, L.F. Effect of BAP concentrations in pre-culture medium and BAP and TDZ in sub-culture medium on coffee micropropagation |
| description |
The residual effects of BAP [benzyladenine] in a pre-culture medium and BAP and TDZ (thidiazuron) in a sub-culture medium on coffee axillary bud proliferation were evaluated. Nodal segments of coffee cv. Caturra Amarelo established in vitro were pre-cultured in MS medium without the growth regulators or with BAP at 6 mg/litre. After 180 days, the nodal segments were inoculated in MS medium supplemented with BAP at 0, 6, 9, 12 and 15 mg/litre, in the first experiment. In the second experiment, the nodal segments were inoculated in MS medium containing TDZ at 0, 0.05, 0.15, 0.45 and 1.35 mg/litre. The pH of the medium was adjusted to 5.8 and solidified with 0.7 |
| format |
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| topic_facet |
COFFEA COFFEA ARABICA 6-BENZILADENINA YEMA (PLANTA) CAFE MEDIO DE CULTIVO CULTIVO IN VITRO MICROPROPAGACION SUSTANCIAS DE CRECIMIENTO VEGETAL EFECTOS RESIDUALES BROTACION TIDIAZURON CULTIVO DE TEJIDOS |
| author |
80886 Jesús, A.M.S. 54822 Carvalho, S.P. de 103528 Pasqual, M. 54799 Carvalho, M. 63857 Dutra, L.F. |
| author_facet |
80886 Jesús, A.M.S. 54822 Carvalho, S.P. de 103528 Pasqual, M. 54799 Carvalho, M. 63857 Dutra, L.F. |
| author_sort |
80886 Jesús, A.M.S. |
| title |
Effect of BAP concentrations in pre-culture medium and BAP and TDZ in sub-culture medium on coffee micropropagation |
| title_short |
Effect of BAP concentrations in pre-culture medium and BAP and TDZ in sub-culture medium on coffee micropropagation |
| title_full |
Effect of BAP concentrations in pre-culture medium and BAP and TDZ in sub-culture medium on coffee micropropagation |
| title_fullStr |
Effect of BAP concentrations in pre-culture medium and BAP and TDZ in sub-culture medium on coffee micropropagation |
| title_full_unstemmed |
Effect of BAP concentrations in pre-culture medium and BAP and TDZ in sub-culture medium on coffee micropropagation |
| title_sort |
effect of bap concentrations in pre-culture medium and bap and tdz in sub-culture medium on coffee micropropagation |
| publishDate |
2002 |
| work_keys_str_mv |
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| _version_ |
1756059918256832513 |