Colchicine-induced microspore embryogenesis in coffee
A protocol for the induction of androgenesis and plant regeneration from C. arabica cv. Caturra isolated microspores in vitro using colchicine pretreatment has been developed. Microspores were mechanically isolated and then carefully purified. Before colchicine pretreatment, microspores were cultured in a semi-solid medium for further develop and regeneration. Different times of colchicine exposure as well as different concentrations were tested. The best androgenic response was found when microspores were precultured in 100 mg l-1 colchicine for 48 h. The microspore developmental stages responsive to colchicine were late-uninucleated and early binucleated pollen. Flow cytometry and morphological analyses revealed that 95 percent of regenerated plants weredihaploids (2n=2x=22). However, some doubled dihaploid plants (2n=4x=44) were also obtained, suggesting that not only androgenic induction but also chromosome duplication could be expected as result of colchicine exposure of coffee microspores. This report represents a new approach in the coffee pollen culture, as well as a major step forward to the utilization of haploid technology in coffee breeding.
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2002
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Subjects: | COFFEA, COFFEA ARABICA, ANDROGENESIS, CULTIVO DE ANTERAS, CROMOSOMAS, CAFE, COLCHICINA, MEDIO DE CULTIVO, DESARROLLO EMBRIONARIO, CULTIVO IN VITRO, METODOS, |
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KOHA-OAI-BVE:891492020-02-03T21:57:00ZColchicine-induced microspore embryogenesis in coffee 77694 Herrera, J.C. 96600 Moreno, L.G. 40014 Acuña, J.R. 104918 Peña, M. de 101988 Osorio, D. 2002A protocol for the induction of androgenesis and plant regeneration from C. arabica cv. Caturra isolated microspores in vitro using colchicine pretreatment has been developed. Microspores were mechanically isolated and then carefully purified. Before colchicine pretreatment, microspores were cultured in a semi-solid medium for further develop and regeneration. Different times of colchicine exposure as well as different concentrations were tested. The best androgenic response was found when microspores were precultured in 100 mg l-1 colchicine for 48 h. The microspore developmental stages responsive to colchicine were late-uninucleated and early binucleated pollen. Flow cytometry and morphological analyses revealed that 95 percent of regenerated plants weredihaploids (2n=2x=22). However, some doubled dihaploid plants (2n=4x=44) were also obtained, suggesting that not only androgenic induction but also chromosome duplication could be expected as result of colchicine exposure of coffee microspores. This report represents a new approach in the coffee pollen culture, as well as a major step forward to the utilization of haploid technology in coffee breeding.A protocol for the induction of androgenesis and plant regeneration from C. arabica cv. Caturra isolated microspores in vitro using colchicine pretreatment has been developed. Microspores were mechanically isolated and then carefully purified. Before colchicine pretreatment, microspores were cultured in a semi-solid medium for further develop and regeneration. Different times of colchicine exposure as well as different concentrations were tested. The best androgenic response was found when microspores were precultured in 100 mg l-1 colchicine for 48 h. The microspore developmental stages responsive to colchicine were late-uninucleated and early binucleated pollen. Flow cytometry and morphological analyses revealed that 95 percent of regenerated plants weredihaploids (2n=2x=22). However, some doubled dihaploid plants (2n=4x=44) were also obtained, suggesting that not only androgenic induction but also chromosome duplication could be expected as result of colchicine exposure of coffee microspores. This report represents a new approach in the coffee pollen culture, as well as a major step forward to the utilization of haploid technology in coffee breeding.COFFEACOFFEA ARABICAANDROGENESISCULTIVO DE ANTERASCROMOSOMASCAFECOLCHICINAMEDIO DE CULTIVODESARROLLO EMBRIONARIOCULTIVO IN VITROMETODOSPlant Cell, Tissue and Organ Culture (Países Bajos) |
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COFFEA COFFEA ARABICA ANDROGENESIS CULTIVO DE ANTERAS CROMOSOMAS CAFE COLCHICINA MEDIO DE CULTIVO DESARROLLO EMBRIONARIO CULTIVO IN VITRO METODOS COFFEA COFFEA ARABICA ANDROGENESIS CULTIVO DE ANTERAS CROMOSOMAS CAFE COLCHICINA MEDIO DE CULTIVO DESARROLLO EMBRIONARIO CULTIVO IN VITRO METODOS |
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COFFEA COFFEA ARABICA ANDROGENESIS CULTIVO DE ANTERAS CROMOSOMAS CAFE COLCHICINA MEDIO DE CULTIVO DESARROLLO EMBRIONARIO CULTIVO IN VITRO METODOS COFFEA COFFEA ARABICA ANDROGENESIS CULTIVO DE ANTERAS CROMOSOMAS CAFE COLCHICINA MEDIO DE CULTIVO DESARROLLO EMBRIONARIO CULTIVO IN VITRO METODOS 77694 Herrera, J.C. 96600 Moreno, L.G. 40014 Acuña, J.R. 104918 Peña, M. de 101988 Osorio, D. Colchicine-induced microspore embryogenesis in coffee |
description |
A protocol for the induction of androgenesis and plant regeneration from C. arabica cv. Caturra isolated microspores in vitro using colchicine pretreatment has been developed. Microspores were mechanically isolated and then carefully purified. Before colchicine pretreatment, microspores were cultured in a semi-solid medium for further develop and regeneration. Different times of colchicine exposure as well as different concentrations were tested. The best androgenic response was found when microspores were precultured in 100 mg l-1 colchicine for 48 h. The microspore developmental stages responsive to colchicine were late-uninucleated and early binucleated pollen. Flow cytometry and morphological analyses revealed that 95 percent of regenerated plants weredihaploids (2n=2x=22). However, some doubled dihaploid plants (2n=4x=44) were also obtained, suggesting that not only androgenic induction but also chromosome duplication could be expected as result of colchicine exposure of coffee microspores. This report represents a new approach in the coffee pollen culture, as well as a major step forward to the utilization of haploid technology in coffee breeding. |
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topic_facet |
COFFEA COFFEA ARABICA ANDROGENESIS CULTIVO DE ANTERAS CROMOSOMAS CAFE COLCHICINA MEDIO DE CULTIVO DESARROLLO EMBRIONARIO CULTIVO IN VITRO METODOS |
author |
77694 Herrera, J.C. 96600 Moreno, L.G. 40014 Acuña, J.R. 104918 Peña, M. de 101988 Osorio, D. |
author_facet |
77694 Herrera, J.C. 96600 Moreno, L.G. 40014 Acuña, J.R. 104918 Peña, M. de 101988 Osorio, D. |
author_sort |
77694 Herrera, J.C. |
title |
Colchicine-induced microspore embryogenesis in coffee |
title_short |
Colchicine-induced microspore embryogenesis in coffee |
title_full |
Colchicine-induced microspore embryogenesis in coffee |
title_fullStr |
Colchicine-induced microspore embryogenesis in coffee |
title_full_unstemmed |
Colchicine-induced microspore embryogenesis in coffee |
title_sort |
colchicine-induced microspore embryogenesis in coffee |
publishDate |
2002 |
work_keys_str_mv |
AT 77694herrerajc colchicineinducedmicrosporeembryogenesisincoffee AT 96600morenolg colchicineinducedmicrosporeembryogenesisincoffee AT 40014acunajr colchicineinducedmicrosporeembryogenesisincoffee AT 104918penamde colchicineinducedmicrosporeembryogenesisincoffee AT 101988osoriod colchicineinducedmicrosporeembryogenesisincoffee |
_version_ |
1756059915996102656 |