Effect of embryo and exogenous GA3 on endospermic endo-{DF}-mannase activity of Coffea arabica L. during germination and early seedling growth

Endo-ß-mannanase (EC 3.2.1.78) is produced by the cells of the endosperm of coffee (Coffea arabica L.) seeds. During germination of intact seeds the enzyme activity was not detected prior to radicle emergence (0-10 days) and increased several fold over the following stage of radicle emergence and post- radicle emergence (10-25 days). Enzyme activity increases from the micropylar end to the opposite end of the seed and correlates with the growth of the cotyledons. When endosperms are isolated just prior to the time when radicle emergence begins (8th day) and left intact. or incubated in a large volume of water for 48 h, no endo-ß-mannanase activity is detected over the following period. When isolated endosperms are incubated in the presence of growth regulators only gibberellic acid promotes an enhancement of enzyme activity. Abscisic acid or cycloheximide completely inhibit endo-ß-mannanase production in intact germinating seeds or inhibit the promoting effect of gibberellic acid in isolated endosperms. It is concluded that the activity of endo-ß-mannanase, and thus the mobilization of galactomannan in C. arabica seed, is essentially a post-germinative phenomenon and may be under the strict control of the embryo-axis. Possible roles of gibberellins and ABA are discussed.

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Main Authors: 71868 Giorgini, J.F., 58507 Comoli, E.
Format: biblioteca
Published: Abr
Subjects:COFFEA ARABICA, SEMILLA, ENDOSPERMA, AMINOAZUCARES, GERMINACION, PLANTULAS,
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spelling KOHA-OAI-BVE:868462020-02-03T21:54:18ZEffect of embryo and exogenous GA3 on endospermic endo-{DF}-mannase activity of Coffea arabica L. during germination and early seedling growth 71868 Giorgini, J.F. 58507 Comoli, E. Abr 1996Endo-ß-mannanase (EC 3.2.1.78) is produced by the cells of the endosperm of coffee (Coffea arabica L.) seeds. During germination of intact seeds the enzyme activity was not detected prior to radicle emergence (0-10 days) and increased several fold over the following stage of radicle emergence and post- radicle emergence (10-25 days). Enzyme activity increases from the micropylar end to the opposite end of the seed and correlates with the growth of the cotyledons. When endosperms are isolated just prior to the time when radicle emergence begins (8th day) and left intact. or incubated in a large volume of water for 48 h, no endo-ß-mannanase activity is detected over the following period. When isolated endosperms are incubated in the presence of growth regulators only gibberellic acid promotes an enhancement of enzyme activity. Abscisic acid or cycloheximide completely inhibit endo-ß-mannanase production in intact germinating seeds or inhibit the promoting effect of gibberellic acid in isolated endosperms. It is concluded that the activity of endo-ß-mannanase, and thus the mobilization of galactomannan in C. arabica seed, is essentially a post-germinative phenomenon and may be under the strict control of the embryo-axis. Possible roles of gibberellins and ABA are discussed.Endo-ß-mannanase (EC 3.2.1.78) is produced by the cells of the endosperm of coffee (Coffea arabica L.) seeds. During germination of intact seeds the enzyme activity was not detected prior to radicle emergence (0-10 days) and increased several fold over the following stage of radicle emergence and post- radicle emergence (10-25 days). Enzyme activity increases from the micropylar end to the opposite end of the seed and correlates with the growth of the cotyledons. When endosperms are isolated just prior to the time when radicle emergence begins (8th day) and left intact. or incubated in a large volume of water for 48 h, no endo-ß-mannanase activity is detected over the following period. When isolated endosperms are incubated in the presence of growth regulators only gibberellic acid promotes an enhancement of enzyme activity. Abscisic acid or cycloheximide completely inhibit endo-ß-mannanase production in intact germinating seeds or inhibit the promoting effect of gibberellic acid in isolated endosperms. It is concluded that the activity of endo-ß-mannanase, and thus the mobilization of galactomannan in C. arabica seed, is essentially a post-germinative phenomenon and may be under the strict control of the embryo-axis. Possible roles of gibberellins and ABA are discussed.COFFEA ARABICASEMILLAENDOSPERMAAMINOAZUCARESGERMINACIONPLANTULASRevista Brasileira de Fisiología Vegetal (Brasil)
institution IICA
collection Koha
country Costa Rica
countrycode CR
component Bibliográfico
access En linea
databasecode cat-sibiica
tag biblioteca
region America Central
libraryname Sistema de Bibliotecas IICA/CATIE
topic COFFEA ARABICA
SEMILLA
ENDOSPERMA
AMINOAZUCARES
GERMINACION
PLANTULAS
COFFEA ARABICA
SEMILLA
ENDOSPERMA
AMINOAZUCARES
GERMINACION
PLANTULAS
spellingShingle COFFEA ARABICA
SEMILLA
ENDOSPERMA
AMINOAZUCARES
GERMINACION
PLANTULAS
COFFEA ARABICA
SEMILLA
ENDOSPERMA
AMINOAZUCARES
GERMINACION
PLANTULAS
71868 Giorgini, J.F.
58507 Comoli, E.
Effect of embryo and exogenous GA3 on endospermic endo-{DF}-mannase activity of Coffea arabica L. during germination and early seedling growth
description Endo-ß-mannanase (EC 3.2.1.78) is produced by the cells of the endosperm of coffee (Coffea arabica L.) seeds. During germination of intact seeds the enzyme activity was not detected prior to radicle emergence (0-10 days) and increased several fold over the following stage of radicle emergence and post- radicle emergence (10-25 days). Enzyme activity increases from the micropylar end to the opposite end of the seed and correlates with the growth of the cotyledons. When endosperms are isolated just prior to the time when radicle emergence begins (8th day) and left intact. or incubated in a large volume of water for 48 h, no endo-ß-mannanase activity is detected over the following period. When isolated endosperms are incubated in the presence of growth regulators only gibberellic acid promotes an enhancement of enzyme activity. Abscisic acid or cycloheximide completely inhibit endo-ß-mannanase production in intact germinating seeds or inhibit the promoting effect of gibberellic acid in isolated endosperms. It is concluded that the activity of endo-ß-mannanase, and thus the mobilization of galactomannan in C. arabica seed, is essentially a post-germinative phenomenon and may be under the strict control of the embryo-axis. Possible roles of gibberellins and ABA are discussed.
format
topic_facet COFFEA ARABICA
SEMILLA
ENDOSPERMA
AMINOAZUCARES
GERMINACION
PLANTULAS
author 71868 Giorgini, J.F.
58507 Comoli, E.
author_facet 71868 Giorgini, J.F.
58507 Comoli, E.
author_sort 71868 Giorgini, J.F.
title Effect of embryo and exogenous GA3 on endospermic endo-{DF}-mannase activity of Coffea arabica L. during germination and early seedling growth
title_short Effect of embryo and exogenous GA3 on endospermic endo-{DF}-mannase activity of Coffea arabica L. during germination and early seedling growth
title_full Effect of embryo and exogenous GA3 on endospermic endo-{DF}-mannase activity of Coffea arabica L. during germination and early seedling growth
title_fullStr Effect of embryo and exogenous GA3 on endospermic endo-{DF}-mannase activity of Coffea arabica L. during germination and early seedling growth
title_full_unstemmed Effect of embryo and exogenous GA3 on endospermic endo-{DF}-mannase activity of Coffea arabica L. during germination and early seedling growth
title_sort effect of embryo and exogenous ga3 on endospermic endo-{df}-mannase activity of coffea arabica l. during germination and early seedling growth
publishDate Abr
work_keys_str_mv AT 71868giorginijf effectofembryoandexogenousga3onendospermicendodfmannaseactivityofcoffeaarabicalduringgerminationandearlyseedlinggrowth
AT 58507comolie effectofembryoandexogenousga3onendospermicendodfmannaseactivityofcoffeaarabicalduringgerminationandearlyseedlinggrowth
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