Formation of somatic embryos from protoplasts of Coffea arabica L.
Somatic embryos were regenerated from protoplasts isolated from embryogenic callus on young leaf explants from mature coffee trees. Embryos were regenerated on modified Murashige and Skoog medium supplemented with 5 uM BA. Somatic embryos developed into intact plants. Mannitol at 0.5 M was adequate as an osmoticum for isolating protoplasts, but subsequent culture required 0.3 M mannitol. A culture system in which osmolality was decreased gradually accelerated formation of colonies and somatic embryogenesis. Chemical name used: N-(phenylmethyl)-1H-purine-6-amine (BA).
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1994
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| Subjects: | COFFEA ARABICA, EMBRION SOMATICO, PROTOPLASTOS, CULTIVO DE CELULAS, PROPAGACION VEGETATIVA, CULTIVO DE TEJIDOS, MICROPROPAGACION, CITOCININAS, |
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KOHA-OAI-BVE:861122020-02-03T21:53:30ZFormation of somatic embryos from protoplasts of Coffea arabica L. 123205 Tahara, M. 132467 Yasuda, T. 126002 Uchida, N. 132384 Yamaguchi, T. 1994Somatic embryos were regenerated from protoplasts isolated from embryogenic callus on young leaf explants from mature coffee trees. Embryos were regenerated on modified Murashige and Skoog medium supplemented with 5 uM BA. Somatic embryos developed into intact plants. Mannitol at 0.5 M was adequate as an osmoticum for isolating protoplasts, but subsequent culture required 0.3 M mannitol. A culture system in which osmolality was decreased gradually accelerated formation of colonies and somatic embryogenesis. Chemical name used: N-(phenylmethyl)-1H-purine-6-amine (BA).Somatic embryos were regenerated from protoplasts isolated from embryogenic callus on young leaf explants from mature coffee trees. Embryos were regenerated on modified Murashige and Skoog medium supplemented with 5 uM BA. Somatic embryos developed into intact plants. Mannitol at 0.5 M was adequate as an osmoticum for isolating protoplasts, but subsequent culture required 0.3 M mannitol. A culture system in which osmolality was decreased gradually accelerated formation of colonies and somatic embryogenesis. Chemical name used: N-(phenylmethyl)-1H-purine-6-amine (BA).COFFEA ARABICAEMBRION SOMATICOPROTOPLASTOSCULTIVO DE CELULASPROPAGACION VEGETATIVACULTIVO DE TEJIDOSMICROPROPAGACIONCITOCININASHortScience (EUA) |
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Costa Rica |
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CR |
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biblioteca |
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America Central |
| libraryname |
Sistema de Bibliotecas IICA/CATIE |
| topic |
COFFEA ARABICA EMBRION SOMATICO PROTOPLASTOS CULTIVO DE CELULAS PROPAGACION VEGETATIVA CULTIVO DE TEJIDOS MICROPROPAGACION CITOCININAS COFFEA ARABICA EMBRION SOMATICO PROTOPLASTOS CULTIVO DE CELULAS PROPAGACION VEGETATIVA CULTIVO DE TEJIDOS MICROPROPAGACION CITOCININAS |
| spellingShingle |
COFFEA ARABICA EMBRION SOMATICO PROTOPLASTOS CULTIVO DE CELULAS PROPAGACION VEGETATIVA CULTIVO DE TEJIDOS MICROPROPAGACION CITOCININAS COFFEA ARABICA EMBRION SOMATICO PROTOPLASTOS CULTIVO DE CELULAS PROPAGACION VEGETATIVA CULTIVO DE TEJIDOS MICROPROPAGACION CITOCININAS 123205 Tahara, M. 132467 Yasuda, T. 126002 Uchida, N. 132384 Yamaguchi, T. Formation of somatic embryos from protoplasts of Coffea arabica L. |
| description |
Somatic embryos were regenerated from protoplasts isolated from embryogenic callus on young leaf explants from mature coffee trees. Embryos were regenerated on modified Murashige and Skoog medium supplemented with 5 uM BA. Somatic embryos developed into intact plants. Mannitol at 0.5 M was adequate as an osmoticum for isolating protoplasts, but subsequent culture required 0.3 M mannitol. A culture system in which osmolality was decreased gradually accelerated formation of colonies and somatic embryogenesis. Chemical name used: N-(phenylmethyl)-1H-purine-6-amine (BA). |
| format |
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| topic_facet |
COFFEA ARABICA EMBRION SOMATICO PROTOPLASTOS CULTIVO DE CELULAS PROPAGACION VEGETATIVA CULTIVO DE TEJIDOS MICROPROPAGACION CITOCININAS |
| author |
123205 Tahara, M. 132467 Yasuda, T. 126002 Uchida, N. 132384 Yamaguchi, T. |
| author_facet |
123205 Tahara, M. 132467 Yasuda, T. 126002 Uchida, N. 132384 Yamaguchi, T. |
| author_sort |
123205 Tahara, M. |
| title |
Formation of somatic embryos from protoplasts of Coffea arabica L. |
| title_short |
Formation of somatic embryos from protoplasts of Coffea arabica L. |
| title_full |
Formation of somatic embryos from protoplasts of Coffea arabica L. |
| title_fullStr |
Formation of somatic embryos from protoplasts of Coffea arabica L. |
| title_full_unstemmed |
Formation of somatic embryos from protoplasts of Coffea arabica L. |
| title_sort |
formation of somatic embryos from protoplasts of coffea arabica l. |
| publishDate |
1994 |
| work_keys_str_mv |
AT 123205taharam formationofsomaticembryosfromprotoplastsofcoffeaarabical AT 132467yasudat formationofsomaticembryosfromprotoplastsofcoffeaarabical AT 126002uchidan formationofsomaticembryosfromprotoplastsofcoffeaarabical AT 132384yamaguchit formationofsomaticembryosfromprotoplastsofcoffeaarabical |
| _version_ |
1756059504232890368 |