Studies on the metabolic control of caffeine turnover in developing endosperms and leaves of Coffea arabica and Coffea dewevrei
Leaves and immature fruits of Coffea dewevrei and Coffea arabica var. Mundo Novo and var. Laurina were fed with [8 exponent -3 H] caffeine and the products analyzed by reversed-phase high-performance liquid chromatography with ultraviolet and radioactivity detection. In fruits, only endosperms were analyzed. In addition, the activities of two methyltransferases involved in the biosynthesis of caffeine were studied in cell-free preparations from leaves and endosperms. The results provide evidence that the low caffeine content in leaves and endosperms of immature fruits of C. dewevrei may be due to slow biosynthesis and fast degradation. The high caffeine content in leaves and endosperms of C. arabica seems to be a consequence of a rapid rate of production coupled with a slow degradation rate.
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | biblioteca |
| Published: |
1994
|
| Subjects: | COFFEA ARABICA, COFFEA DEWEVREI, HOJAS, ENDOSPERMA, CAFEINA, CROMATOGRAFIA, CROMATOGRAFIA LIQUIDA ALTA PRESION, TECNICAS ANALITICAS, |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
KOHA-OAI-BVE:86101 |
|---|---|
| record_format |
koha |
| spelling |
KOHA-OAI-BVE:861012020-02-03T21:53:29ZStudies on the metabolic control of caffeine turnover in developing endosperms and leaves of Coffea arabica and Coffea dewevrei 92133 Mazzafera, P. 60001 Crozier, A. 115710 Sandberg, G. 1994Leaves and immature fruits of Coffea dewevrei and Coffea arabica var. Mundo Novo and var. Laurina were fed with [8 exponent -3 H] caffeine and the products analyzed by reversed-phase high-performance liquid chromatography with ultraviolet and radioactivity detection. In fruits, only endosperms were analyzed. In addition, the activities of two methyltransferases involved in the biosynthesis of caffeine were studied in cell-free preparations from leaves and endosperms. The results provide evidence that the low caffeine content in leaves and endosperms of immature fruits of C. dewevrei may be due to slow biosynthesis and fast degradation. The high caffeine content in leaves and endosperms of C. arabica seems to be a consequence of a rapid rate of production coupled with a slow degradation rate.Leaves and immature fruits of Coffea dewevrei and Coffea arabica var. Mundo Novo and var. Laurina were fed with [8 exponent -3 H] caffeine and the products analyzed by reversed-phase high-performance liquid chromatography with ultraviolet and radioactivity detection. In fruits, only endosperms were analyzed. In addition, the activities of two methyltransferases involved in the biosynthesis of caffeine were studied in cell-free preparations from leaves and endosperms. The results provide evidence that the low caffeine content in leaves and endosperms of immature fruits of C. dewevrei may be due to slow biosynthesis and fast degradation. The high caffeine content in leaves and endosperms of C. arabica seems to be a consequence of a rapid rate of production coupled with a slow degradation rate.COFFEA ARABICACOFFEA DEWEVREIHOJASENDOSPERMACAFEINACROMATOGRAFIACROMATOGRAFIA LIQUIDA ALTA PRESIONTECNICAS ANALITICASJournal of Agricultural and Food Chemistry (EUA) |
| institution |
IICA |
| collection |
Koha |
| country |
Costa Rica |
| countrycode |
CR |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
cat-sibiica |
| tag |
biblioteca |
| region |
America Central |
| libraryname |
Sistema de Bibliotecas IICA/CATIE |
| topic |
COFFEA ARABICA COFFEA DEWEVREI HOJAS ENDOSPERMA CAFEINA CROMATOGRAFIA CROMATOGRAFIA LIQUIDA ALTA PRESION TECNICAS ANALITICAS COFFEA ARABICA COFFEA DEWEVREI HOJAS ENDOSPERMA CAFEINA CROMATOGRAFIA CROMATOGRAFIA LIQUIDA ALTA PRESION TECNICAS ANALITICAS |
| spellingShingle |
COFFEA ARABICA COFFEA DEWEVREI HOJAS ENDOSPERMA CAFEINA CROMATOGRAFIA CROMATOGRAFIA LIQUIDA ALTA PRESION TECNICAS ANALITICAS COFFEA ARABICA COFFEA DEWEVREI HOJAS ENDOSPERMA CAFEINA CROMATOGRAFIA CROMATOGRAFIA LIQUIDA ALTA PRESION TECNICAS ANALITICAS 92133 Mazzafera, P. 60001 Crozier, A. 115710 Sandberg, G. Studies on the metabolic control of caffeine turnover in developing endosperms and leaves of Coffea arabica and Coffea dewevrei |
| description |
Leaves and immature fruits of Coffea dewevrei and Coffea arabica var. Mundo Novo and var. Laurina were fed with [8 exponent -3 H] caffeine and the products analyzed by reversed-phase high-performance liquid chromatography with ultraviolet and radioactivity detection. In fruits, only endosperms were analyzed. In addition, the activities of two methyltransferases involved in the biosynthesis of caffeine were studied in cell-free preparations from leaves and endosperms. The results provide evidence that the low caffeine content in leaves and endosperms of immature fruits of C. dewevrei may be due to slow biosynthesis and fast degradation. The high caffeine content in leaves and endosperms of C. arabica seems to be a consequence of a rapid rate of production coupled with a slow degradation rate. |
| format |
|
| topic_facet |
COFFEA ARABICA COFFEA DEWEVREI HOJAS ENDOSPERMA CAFEINA CROMATOGRAFIA CROMATOGRAFIA LIQUIDA ALTA PRESION TECNICAS ANALITICAS |
| author |
92133 Mazzafera, P. 60001 Crozier, A. 115710 Sandberg, G. |
| author_facet |
92133 Mazzafera, P. 60001 Crozier, A. 115710 Sandberg, G. |
| author_sort |
92133 Mazzafera, P. |
| title |
Studies on the metabolic control of caffeine turnover in developing endosperms and leaves of Coffea arabica and Coffea dewevrei |
| title_short |
Studies on the metabolic control of caffeine turnover in developing endosperms and leaves of Coffea arabica and Coffea dewevrei |
| title_full |
Studies on the metabolic control of caffeine turnover in developing endosperms and leaves of Coffea arabica and Coffea dewevrei |
| title_fullStr |
Studies on the metabolic control of caffeine turnover in developing endosperms and leaves of Coffea arabica and Coffea dewevrei |
| title_full_unstemmed |
Studies on the metabolic control of caffeine turnover in developing endosperms and leaves of Coffea arabica and Coffea dewevrei |
| title_sort |
studies on the metabolic control of caffeine turnover in developing endosperms and leaves of coffea arabica and coffea dewevrei |
| publishDate |
1994 |
| work_keys_str_mv |
AT 92133mazzaferap studiesonthemetaboliccontrolofcaffeineturnoverindevelopingendospermsandleavesofcoffeaarabicaandcoffeadewevrei AT 60001croziera studiesonthemetaboliccontrolofcaffeineturnoverindevelopingendospermsandleavesofcoffeaarabicaandcoffeadewevrei AT 115710sandbergg studiesonthemetaboliccontrolofcaffeineturnoverindevelopingendospermsandleavesofcoffeaarabicaandcoffeadewevrei |
| _version_ |
1756059502771175424 |