Somatic embryogenesis and regeneration of plantlets in protoplast cultures from somatic embryos of coffee (Coffea canephora P. ex Fr)

Somatic embryogenesis and regeneration of plantlets in protoplast cultures from somatic embryos of coffee (Coffea canephora P. ex Fr)

Somatic embryogenesis was achieved in protoplast cultures of coffee. Protoplasts were isolated from cell suspension-derived somatic embryos of Coffea canephora. After repeated subculture in a medium supplemented with 0.5 mg/1 of each of kinetin, 2,4-dichloro-phenoxyacetic acid (2,4-D), and naphthaleneacetic acid (NAA), microcalli developed. Transfer of these microcalli to a medium lacking growth regulators resulted in the formation of globular embryos. Upon subculture without growth regulators they grew to well-differentiated embryos, eventually some of them developed to plantlets which were transferred to the greenhouse for further observation. (ICO. Library Monthly Entries no. 84:15. 1987)

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Bibliographic Details
Main Author: 117139 Schopke, C.
Format: biblioteca
Published: 1987
Subjects:COFFEA CANEPHORA, EMBRIOGENESIS SOMATICA, PROTOPLASTOS, CULTIVO DE PROTOPLASTOS,
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id KOHA-OAI-BVE:85429
record_format koha
spelling KOHA-OAI-BVE:854292020-02-03T21:52:44ZSomatic embryogenesis and regeneration of plantlets in protoplast cultures from somatic embryos of coffee (Coffea canephora P. ex Fr) 117139 Schopke, C. 1987Somatic embryogenesis was achieved in protoplast cultures of coffee. Protoplasts were isolated from cell suspension-derived somatic embryos of Coffea canephora. After repeated subculture in a medium supplemented with 0.5 mg/1 of each of kinetin, 2,4-dichloro-phenoxyacetic acid (2,4-D), and naphthaleneacetic acid (NAA), microcalli developed. Transfer of these microcalli to a medium lacking growth regulators resulted in the formation of globular embryos. Upon subculture without growth regulators they grew to well-differentiated embryos, eventually some of them developed to plantlets which were transferred to the greenhouse for further observation. (ICO. Library Monthly Entries no. 84:15. 1987)Somatic embryogenesis was achieved in protoplast cultures of coffee. Protoplasts were isolated from cell suspension-derived somatic embryos of Coffea canephora. After repeated subculture in a medium supplemented with 0.5 mg/1 of each of kinetin, 2,4-dichloro-phenoxyacetic acid (2,4-D), and naphthaleneacetic acid (NAA), microcalli developed. Transfer of these microcalli to a medium lacking growth regulators resulted in the formation of globular embryos. Upon subculture without growth regulators they grew to well-differentiated embryos, eventually some of them developed to plantlets which were transferred to the greenhouse for further observation. (ICO. Library Monthly Entries no. 84:15. 1987)COFFEA CANEPHORAEMBRIOGENESIS SOMATICAPROTOPLASTOSCULTIVO DE PROTOPLASTOSPlant Cell, Tissue and Organ Culture (Países Bajos)
institution IICA
collection Koha
country Costa Rica
countrycode CR
component Bibliográfico
access En linea
databasecode cat-sibiica
tag biblioteca
region America Central
libraryname Sistema de Bibliotecas IICA/CATIE
topic COFFEA CANEPHORA
EMBRIOGENESIS SOMATICA
PROTOPLASTOS
CULTIVO DE PROTOPLASTOS
COFFEA CANEPHORA
EMBRIOGENESIS SOMATICA
PROTOPLASTOS
CULTIVO DE PROTOPLASTOS
spellingShingle COFFEA CANEPHORA
EMBRIOGENESIS SOMATICA
PROTOPLASTOS
CULTIVO DE PROTOPLASTOS
COFFEA CANEPHORA
EMBRIOGENESIS SOMATICA
PROTOPLASTOS
CULTIVO DE PROTOPLASTOS
117139 Schopke, C.
Somatic embryogenesis and regeneration of plantlets in protoplast cultures from somatic embryos of coffee (Coffea canephora P. ex Fr)
description Somatic embryogenesis was achieved in protoplast cultures of coffee. Protoplasts were isolated from cell suspension-derived somatic embryos of Coffea canephora. After repeated subculture in a medium supplemented with 0.5 mg/1 of each of kinetin, 2,4-dichloro-phenoxyacetic acid (2,4-D), and naphthaleneacetic acid (NAA), microcalli developed. Transfer of these microcalli to a medium lacking growth regulators resulted in the formation of globular embryos. Upon subculture without growth regulators they grew to well-differentiated embryos, eventually some of them developed to plantlets which were transferred to the greenhouse for further observation. (ICO. Library Monthly Entries no. 84:15. 1987)
format
topic_facet COFFEA CANEPHORA
EMBRIOGENESIS SOMATICA
PROTOPLASTOS
CULTIVO DE PROTOPLASTOS
author 117139 Schopke, C.
author_facet 117139 Schopke, C.
author_sort 117139 Schopke, C.
title Somatic embryogenesis and regeneration of plantlets in protoplast cultures from somatic embryos of coffee (Coffea canephora P. ex Fr)
title_short Somatic embryogenesis and regeneration of plantlets in protoplast cultures from somatic embryos of coffee (Coffea canephora P. ex Fr)
title_full Somatic embryogenesis and regeneration of plantlets in protoplast cultures from somatic embryos of coffee (Coffea canephora P. ex Fr)
title_fullStr Somatic embryogenesis and regeneration of plantlets in protoplast cultures from somatic embryos of coffee (Coffea canephora P. ex Fr)
title_full_unstemmed Somatic embryogenesis and regeneration of plantlets in protoplast cultures from somatic embryos of coffee (Coffea canephora P. ex Fr)
title_sort somatic embryogenesis and regeneration of plantlets in protoplast cultures from somatic embryos of coffee (coffea canephora p. ex fr)
publishDate 1987
work_keys_str_mv AT 117139schopkec somaticembryogenesisandregenerationofplantletsinprotoplastculturesfromsomaticembryosofcoffeecoffeacanephorapexfr
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