Comunicacoes
In order to have a system for clonal propagation of improved cultivars obtained in the regular breeding programmes, or to regenerate plants from protoplast fusion, one of the first steps of this work was to obtain plant regeneration. This result was achieved by cultivating mature leaf explants of C. canephora and C. arabica c.v. Mundo Novo in a nutrient solid medium for callus proliferation. Afterwards, tissues were subcultured in a liquid medium to start a suspension culture. Embryo formation was obtained eight week later under dark conditions. When the cotyledonary leaf started to develop, the embryos were placed under illumination and developed into green plantlets, which were then subcultured in solid medium until a new taproot was formed. Plants were transplanted in sterile scoria and kept in the growth chamber for a month, and then they were taken to the coffee growing areas where they were transplanted to sterile soil and maintained in an Environmental Room for two months. During this period, the height of the plants increased by three times. Then, the plants were transplanted in small bags with non-sterile soil, and placed under natural conditions. After three months, they developed the first plagiotropic ramification and a very good root system. The plants were then transplanted to the field in order to continue their evaluation. Up to now, the plants have exhibited normal growth, comparable to that of plants obtained from seeds. These preliminary results show that in vitro plant regeneration is a promising technique for the propagation of improved coffee cultivars
| Main Authors: | , , |
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| Format: | biblioteca |
| Language: | spa |
| Published: |
Oeiras (Portugal)
1984
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| Subjects: | COFFEA ARABICA, COFFEA CANEPHORA, EMBRIOGENESIS SOMATICA, CLONES, EXPLANTES, CALLO, CULTIVO DE TEJIDOS, |
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