A technique for enhancing spore germination of Moniliophthora roreri
A technique was devised for obtaining consistently a high percentage germination of conidia of Moniliophthora roreri, agent of moniliasis of cacao. A suspension (10 exponent 6 conidia/ml) was prepared aseptically in sterile distilled water from an isolate grown for 15 days on potato carrot dextrose agar at 25 grade centigrade. Drops of this suspension were placed on prepoured (24 h) plates of Yeast Extract Agar (Oxoid Yeast extract 1 percent, Difco agar 1.5 percent) which were then incubated at lab. temperature (23-25 grade centigrade). Germination was assessed at intervals in samples stained with trypan blue or diluted cotton blue. Germination was observed after 4 h and c. 80 percent conidia germinated within 12 h, most by a single germ-tube. By 24 h mycelium had developed. Further tests showed that germination varied with age, isolate, pH and temperature.
Main Authors: | 108494 Ram, A., 131188 Wheeler, B.E.J., 69828 Galindo, J.J., 32334 1987 Annual Meeting of the American Phytopathological Society. Caribbean Div Guatemala (Guatemala) 26-31 Oct 1987 |
---|---|
Format: | biblioteca |
Published: |
Jun
|
Subjects: | THEOBROMA CACAO, MONILIOPHTHORA RORERI, ENFERMEDADES FUNGOSAS, ESPORULACION, ESPORAS, |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Estudio de la germinación de conidios de Moniliophthora roreri en mazorcas de cacao
by: 68131 Flores, D., et al.
Published: (1988) -
Fluctuación de poblaciones de esporas de Monilia roreri Cif. & Par. y viabilidad durante un ciclo completo de afección
by: 53602 Campuzano Londoño, H., et al.
Published: (1976) -
Crecimiento y esporulación de Moniliophthora roreri en varios medios de cultivo compuestos
by: 77603 Herrera M, F., et al.
Published: (Dic ) -
Liberación y dispersión de esporas de Monilia roreri Cif. & Par.
by: 93715 Merchán, V.M., et al.
Published: (1978) -
Estudios sobre Monilia roreri adelantados en Caldas-Colombia
by: 73697 Green, M.J.
Published: (1977)