The use of isozyme analysis in the characterization of cocoa germplasm
Starch gel electrophoresis was performed on bark tissue of Theobroma cacao to generate isoperoxidase banding patterns. The zymograms obtained were used to broadly classify the extensive germplasm collection at the Cocoa Research Unit. Work done on other enzyme systems, a.g. malate dehydrogenase, alcohol dehydrogenase, laccase and phosphoglum comutase is expected to further subdivide the groups already established by peroxidase. The results so far indicate that there are differences in the banding patterns of the sample clones. There is also variation within populations originating from the same geographical site. These patterns will be used to uniquely characterise small groups of related germplasm and assist in a rational exploitation of genetic resources.
Main Authors: | 64848 Elliot, A., 82731 Kennedy, A.J., 15005 National Institute of Higher Education Research, Science and Technology, St. Augustine (Trinidad y Tobago), 20756 University of the West Indies, St. Augustine (Trinidad y Tobago), 14659 Ministry of Food Production, Marine Exploitation, Forestry and the Environment (Trinidad y Tobago), 32355 2. Annual Seminar on Agricultural Research St. Augustine (Trinidad y Tobago) 10-12 Nov 1988 |
---|---|
Format: | biblioteca |
Published: |
St. Augustine (Trinidad y Tobago)
1989
|
Subjects: | THEOBROMA CACAO, GERMOPLASMA, ISOENZIMAS, ANALISIS DE TEJIDOS, COLECCION DE PLANTAS, TRINIDAD Y TOBAGO, |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Germplasm collection
by: 97646 Murray, D.B., et al.
Published: (1977) -
Resistance studies: black pod, witches' broom, Ceratocystis wilt; histological investigations
by: 80035 Iton, E.F., et al.
Published: (1973) -
Germplasm collection
by: 56331 Chalmers, W.S., et al.
Published: (1972) -
Germplasm collection
by: 56331 Chalmers, W.S., et al.
Published: (1973) -
Effective use of cocoa genetic resources as exemplified by the International Cocoa Genebank, Trinidad
by: 125308 Toxopeus, H., et al.
Published: (1989)