High pressure liquid chromatographic determination of theobromine and caffeine in cocoa and chocolate products
A method was developed for determining theobromine and caffeine in cocoa and chocolate products by high pressure liquid chromatography. After a simple hot water extraction, both theobromine and caffeine were separated by using a reverse phase C18 column and a mobile phase of methanol-water-acetic acid (20+79+1). Theobromine and caffeine were quantitated at 280 nm; average recoveries were 98.7 and 95.0 percent; and coefficients of variation were 2.31 and 3.91 percent, respectively.
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1978
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| Subjects: | CACAO, CHOCOLATE, SUBPRODUCTOS, TEOBROMINA, CAFEINA, COMPUESTOS ORGANICOS, CROMATOGRAFIA LIQUIDA ALTA PRESION, ANALISIS QUIMICO, |
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KOHA-OAI-BVE:694662020-02-03T21:36:34ZHigh pressure liquid chromatographic determination of theobromine and caffeine in cocoa and chocolate products 83944 Kreiser, W.R. 90675 Martin Junior, R.A. 1978A method was developed for determining theobromine and caffeine in cocoa and chocolate products by high pressure liquid chromatography. After a simple hot water extraction, both theobromine and caffeine were separated by using a reverse phase C18 column and a mobile phase of methanol-water-acetic acid (20+79+1). Theobromine and caffeine were quantitated at 280 nm; average recoveries were 98.7 and 95.0 percent; and coefficients of variation were 2.31 and 3.91 percent, respectively.A method was developed for determining theobromine and caffeine in cocoa and chocolate products by high pressure liquid chromatography. After a simple hot water extraction, both theobromine and caffeine were separated by using a reverse phase C18 column and a mobile phase of methanol-water-acetic acid (20+79+1). Theobromine and caffeine were quantitated at 280 nm; average recoveries were 98.7 and 95.0 percent; and coefficients of variation were 2.31 and 3.91 percent, respectively.CACAOCHOCOLATESUBPRODUCTOSTEOBROMINACAFEINACOMPUESTOS ORGANICOSCROMATOGRAFIA LIQUIDA ALTA PRESIONANALISIS QUIMICOJournal of the Association of Official Analytical Chemists (EUA) |
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CACAO CHOCOLATE SUBPRODUCTOS TEOBROMINA CAFEINA COMPUESTOS ORGANICOS CROMATOGRAFIA LIQUIDA ALTA PRESION ANALISIS QUIMICO CACAO CHOCOLATE SUBPRODUCTOS TEOBROMINA CAFEINA COMPUESTOS ORGANICOS CROMATOGRAFIA LIQUIDA ALTA PRESION ANALISIS QUIMICO |
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CACAO CHOCOLATE SUBPRODUCTOS TEOBROMINA CAFEINA COMPUESTOS ORGANICOS CROMATOGRAFIA LIQUIDA ALTA PRESION ANALISIS QUIMICO CACAO CHOCOLATE SUBPRODUCTOS TEOBROMINA CAFEINA COMPUESTOS ORGANICOS CROMATOGRAFIA LIQUIDA ALTA PRESION ANALISIS QUIMICO 83944 Kreiser, W.R. 90675 Martin Junior, R.A. High pressure liquid chromatographic determination of theobromine and caffeine in cocoa and chocolate products |
| description |
A method was developed for determining theobromine and caffeine in cocoa and chocolate products by high pressure liquid chromatography. After a simple hot water extraction, both theobromine and caffeine were separated by using a reverse phase C18 column and a mobile phase of methanol-water-acetic acid (20+79+1). Theobromine and caffeine were quantitated at 280 nm; average recoveries were 98.7 and 95.0 percent; and coefficients of variation were 2.31 and 3.91 percent, respectively. |
| format |
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| topic_facet |
CACAO CHOCOLATE SUBPRODUCTOS TEOBROMINA CAFEINA COMPUESTOS ORGANICOS CROMATOGRAFIA LIQUIDA ALTA PRESION ANALISIS QUIMICO |
| author |
83944 Kreiser, W.R. 90675 Martin Junior, R.A. |
| author_facet |
83944 Kreiser, W.R. 90675 Martin Junior, R.A. |
| author_sort |
83944 Kreiser, W.R. |
| title |
High pressure liquid chromatographic determination of theobromine and caffeine in cocoa and chocolate products |
| title_short |
High pressure liquid chromatographic determination of theobromine and caffeine in cocoa and chocolate products |
| title_full |
High pressure liquid chromatographic determination of theobromine and caffeine in cocoa and chocolate products |
| title_fullStr |
High pressure liquid chromatographic determination of theobromine and caffeine in cocoa and chocolate products |
| title_full_unstemmed |
High pressure liquid chromatographic determination of theobromine and caffeine in cocoa and chocolate products |
| title_sort |
high pressure liquid chromatographic determination of theobromine and caffeine in cocoa and chocolate products |
| publishDate |
1978 |
| work_keys_str_mv |
AT 83944kreiserwr highpressureliquidchromatographicdeterminationoftheobromineandcaffeineincocoaandchocolateproducts AT 90675martinjuniorra highpressureliquidchromatographicdeterminationoftheobromineandcaffeineincocoaandchocolateproducts |
| _version_ |
1756057250989867009 |