The purification and some properties of cocoa necrosis virus, a serotype of tomato black ring virus

The purification and some properties of cocoa necrosis virus, a serotype of tomato black ring virus

Cocoa necrosis virus (CNV) was transmitted by sap inoculation to twelve of twenty-one species tested. It was propagated and assayed in Phaseolus vulgaris. Sap from P. vulgaris was infective after dilution to 10 exponent -3 but not 10 exponent -4, after 10 min at 60 centigrade grade but not 65 centigrade grade, and after 4 but not 7 days at 20-24 centigrade grade. Lyophilized sap from P. vulgaris was infective after 2 years in vacuo. Virus was prepared by extracting infected leaves of P. vulgaris with 0.1 M phosphate (pH 7.5) containing 0.05 M ethylene diamine tetra-acetate and 0.02 M thioglycollate. After clarification with n-butanol, virus was purified by precipitation with polyethylene glycol and several cycles of differential centrifugation. Such preparations were very infective and contained numerous particles, 24-26 nm in diameter with a hexagonal profile, which sedimented as two components with sedimentation coefficients (S 20, W) of 101 S and 129 S. The absorption spectra of both components with maximum and minimum absorption at 259 and 240 nm respectively were typical of nucleoproteins (101 S component, A 260/280 = 1.63; A 260/240 = 1.40:129 S component; A 260/280 = 1.78; A 260/240 = 1.58) and indicated nucleic acid contents of ca. 35 per cent for the 129 S component and ca. 20 per cent for the 101 S component; values calculated from the sedimentation coefficients were 41 and 30 per cent respectively. Only the 129 S component seemed to be infective and was not more so when mixed with 101 S component. Both components contained a single protein subunit weighing ca. 60.000 daltons. Under certain conditions sap fractionated without butanol gave virus preparations containing empty protein shells (54 S) and small spherical particles (20-30 S) ca. 12 nm diameter. CNV is a serotype of tomato black ring virus and is distantly related to Hungarian chrome mosaic virus

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Main Author: 82762 Kenten, R.H.
Format: biblioteca
Published: 1972
Subjects:THEOBROMA CACAO, NECROSIS, VIROSIS, DIAGNOSTICO, TECNICAS INMUNOLOGICAS, SAVIA, HUESPEDES,
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spelling KOHA-OAI-BVE:668042020-02-03T21:33:55ZThe purification and some properties of cocoa necrosis virus, a serotype of tomato black ring virus 82762 Kenten, R.H. 1972Cocoa necrosis virus (CNV) was transmitted by sap inoculation to twelve of twenty-one species tested. It was propagated and assayed in Phaseolus vulgaris. Sap from P. vulgaris was infective after dilution to 10 exponent -3 but not 10 exponent -4, after 10 min at 60 centigrade grade but not 65 centigrade grade, and after 4 but not 7 days at 20-24 centigrade grade. Lyophilized sap from P. vulgaris was infective after 2 years in vacuo. Virus was prepared by extracting infected leaves of P. vulgaris with 0.1 M phosphate (pH 7.5) containing 0.05 M ethylene diamine tetra-acetate and 0.02 M thioglycollate. After clarification with n-butanol, virus was purified by precipitation with polyethylene glycol and several cycles of differential centrifugation. Such preparations were very infective and contained numerous particles, 24-26 nm in diameter with a hexagonal profile, which sedimented as two components with sedimentation coefficients (S 20, W) of 101 S and 129 S. The absorption spectra of both components with maximum and minimum absorption at 259 and 240 nm respectively were typical of nucleoproteins (101 S component, A 260/280 = 1.63; A 260/240 = 1.40:129 S component; A 260/280 = 1.78; A 260/240 = 1.58) and indicated nucleic acid contents of ca. 35 per cent for the 129 S component and ca. 20 per cent for the 101 S component; values calculated from the sedimentation coefficients were 41 and 30 per cent respectively. Only the 129 S component seemed to be infective and was not more so when mixed with 101 S component. Both components contained a single protein subunit weighing ca. 60.000 daltons. Under certain conditions sap fractionated without butanol gave virus preparations containing empty protein shells (54 S) and small spherical particles (20-30 S) ca. 12 nm diameter. CNV is a serotype of tomato black ring virus and is distantly related to Hungarian chrome mosaic virusCocoa necrosis virus (CNV) was transmitted by sap inoculation to twelve of twenty-one species tested. It was propagated and assayed in Phaseolus vulgaris. Sap from P. vulgaris was infective after dilution to 10 exponent -3 but not 10 exponent -4, after 10 min at 60 centigrade grade but not 65 centigrade grade, and after 4 but not 7 days at 20-24 centigrade grade. Lyophilized sap from P. vulgaris was infective after 2 years in vacuo. Virus was prepared by extracting infected leaves of P. vulgaris with 0.1 M phosphate (pH 7.5) containing 0.05 M ethylene diamine tetra-acetate and 0.02 M thioglycollate. After clarification with n-butanol, virus was purified by precipitation with polyethylene glycol and several cycles of differential centrifugation. Such preparations were very infective and contained numerous particles, 24-26 nm in diameter with a hexagonal profile, which sedimented as two components with sedimentation coefficients (S 20, W) of 101 S and 129 S. The absorption spectra of both components with maximum and minimum absorption at 259 and 240 nm respectively were typical of nucleoproteins (101 S component, A 260/280 = 1.63; A 260/240 = 1.40:129 S component; A 260/280 = 1.78; A 260/240 = 1.58) and indicated nucleic acid contents of ca. 35 per cent for the 129 S component and ca. 20 per cent for the 101 S component; values calculated from the sedimentation coefficients were 41 and 30 per cent respectively. Only the 129 S component seemed to be infective and was not more so when mixed with 101 S component. Both components contained a single protein subunit weighing ca. 60.000 daltons. Under certain conditions sap fractionated without butanol gave virus preparations containing empty protein shells (54 S) and small spherical particles (20-30 S) ca. 12 nm diameter. CNV is a serotype of tomato black ring virus and is distantly related to Hungarian chrome mosaic virusTHEOBROMA CACAONECROSISVIROSISDIAGNOSTICOTECNICAS INMUNOLOGICASSAVIAHUESPEDESAnnals of Applied Biology (RU)
institution IICA
collection Koha
country Costa Rica
countrycode CR
component Bibliográfico
access En linea
databasecode cat-sibiica
tag biblioteca
region America Central
libraryname Sistema de Bibliotecas IICA/CATIE
topic THEOBROMA CACAO
NECROSIS
VIROSIS
DIAGNOSTICO
TECNICAS INMUNOLOGICAS
SAVIA
HUESPEDES
THEOBROMA CACAO
NECROSIS
VIROSIS
DIAGNOSTICO
TECNICAS INMUNOLOGICAS
SAVIA
HUESPEDES
spellingShingle THEOBROMA CACAO
NECROSIS
VIROSIS
DIAGNOSTICO
TECNICAS INMUNOLOGICAS
SAVIA
HUESPEDES
THEOBROMA CACAO
NECROSIS
VIROSIS
DIAGNOSTICO
TECNICAS INMUNOLOGICAS
SAVIA
HUESPEDES
82762 Kenten, R.H.
The purification and some properties of cocoa necrosis virus, a serotype of tomato black ring virus
description Cocoa necrosis virus (CNV) was transmitted by sap inoculation to twelve of twenty-one species tested. It was propagated and assayed in Phaseolus vulgaris. Sap from P. vulgaris was infective after dilution to 10 exponent -3 but not 10 exponent -4, after 10 min at 60 centigrade grade but not 65 centigrade grade, and after 4 but not 7 days at 20-24 centigrade grade. Lyophilized sap from P. vulgaris was infective after 2 years in vacuo. Virus was prepared by extracting infected leaves of P. vulgaris with 0.1 M phosphate (pH 7.5) containing 0.05 M ethylene diamine tetra-acetate and 0.02 M thioglycollate. After clarification with n-butanol, virus was purified by precipitation with polyethylene glycol and several cycles of differential centrifugation. Such preparations were very infective and contained numerous particles, 24-26 nm in diameter with a hexagonal profile, which sedimented as two components with sedimentation coefficients (S 20, W) of 101 S and 129 S. The absorption spectra of both components with maximum and minimum absorption at 259 and 240 nm respectively were typical of nucleoproteins (101 S component, A 260/280 = 1.63; A 260/240 = 1.40:129 S component; A 260/280 = 1.78; A 260/240 = 1.58) and indicated nucleic acid contents of ca. 35 per cent for the 129 S component and ca. 20 per cent for the 101 S component; values calculated from the sedimentation coefficients were 41 and 30 per cent respectively. Only the 129 S component seemed to be infective and was not more so when mixed with 101 S component. Both components contained a single protein subunit weighing ca. 60.000 daltons. Under certain conditions sap fractionated without butanol gave virus preparations containing empty protein shells (54 S) and small spherical particles (20-30 S) ca. 12 nm diameter. CNV is a serotype of tomato black ring virus and is distantly related to Hungarian chrome mosaic virus
format
topic_facet THEOBROMA CACAO
NECROSIS
VIROSIS
DIAGNOSTICO
TECNICAS INMUNOLOGICAS
SAVIA
HUESPEDES
author 82762 Kenten, R.H.
author_facet 82762 Kenten, R.H.
author_sort 82762 Kenten, R.H.
title The purification and some properties of cocoa necrosis virus, a serotype of tomato black ring virus
title_short The purification and some properties of cocoa necrosis virus, a serotype of tomato black ring virus
title_full The purification and some properties of cocoa necrosis virus, a serotype of tomato black ring virus
title_fullStr The purification and some properties of cocoa necrosis virus, a serotype of tomato black ring virus
title_full_unstemmed The purification and some properties of cocoa necrosis virus, a serotype of tomato black ring virus
title_sort purification and some properties of cocoa necrosis virus, a serotype of tomato black ring virus
publishDate 1972
work_keys_str_mv AT 82762kentenrh thepurificationandsomepropertiesofcocoanecrosisvirusaserotypeoftomatoblackringvirus
AT 82762kentenrh purificationandsomepropertiesofcocoanecrosisvirusaserotypeoftomatoblackringvirus
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